KEGG   ORTHOLOGY: K00296
Entry
K00296                      KO                                     
Symbol
nos
Name
D-nopaline dehydrogenase [EC:1.5.1.19]
Pathway
map00330  Arginine and proline metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K00296  nos; D-nopaline dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.1  With NAD+ or NADP+ as acceptor
    1.5.1.19  D-nopaline dehydrogenase
     K00296  nos; D-nopaline dehydrogenase
Other DBs
RN: R00563
GO: 0047829
Genes
ATU: Atu6015(nos)
AVI: Avi_8195(nos)
ALF: CFBP5473_23905
NEN: NCHU2750_55510(nos)
LinkDB

KEGG   ORTHOLOGY: K24952
Entry
K24952                      KO                                     
Symbol
noxA
Name
nopaline oxidase subunit A
Pathway
map00330  Arginine and proline metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K24952  noxA; nopaline oxidase subunit A
Other DBs
RN: R12706
COG: COG0446
Genes
ATU: Atu6019(noxA)
ARA: Arad_14154(noxA)
ALF: CFBP5473_23930
NEN: NCHU2750_55550(noxA)
Reference
PMID:8045881
  Authors
Zanker H, Lurz G, Langridge U, Langridge P, Kreusch D, Schroder J.
  Title
Octopine and nopaline oxidases from Ti plasmids of Agrobacterium tumefaciens: molecular analysis, relationship, and functional characterization.
  Journal
J Bacteriol 176:4511-7 (1994)
DOI:10.1128/jb.176.15.4511-4517.1994
  Sequence
[atu:Atu6019]
LinkDB

KEGG   ORTHOLOGY: K24953
Entry
K24953                      KO                                     
Symbol
noxB
Name
nopaline oxidase subunit B
Pathway
map00330  Arginine and proline metabolism
map01100  Metabolic pathways
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09105 Amino acid metabolism
   00330 Arginine and proline metabolism
    K24953  noxB; nopaline oxidase subunit B
Other DBs
RN: R12706
COG: COG0665
Genes
ATU: Atu6021(noxB)
ARA: Arad_14155(noxB)
ALF: CFBP5473_23940
NEN: NCHU2750_55570(noxB)
Reference
PMID:8045881
  Authors
Zanker H, Lurz G, Langridge U, Langridge P, Kreusch D, Schroder J.
  Title
Octopine and nopaline oxidases from Ti plasmids of Agrobacterium tumefaciens: molecular analysis, relationship, and functional characterization.
  Journal
J Bacteriol 176:4511-7 (1994)
DOI:10.1128/jb.176.15.4511-4517.1994
  Sequence
[atu:Atu6021]
LinkDB

KEGG   ENZYME: 1.5.1.19
Entry
EC 1.5.1.19                 Enzyme                                 
Name
D-nopaline dehydrogenase;
D-nopaline synthase;
nopaline dehydrogenase;
nopaline synthase;
NOS;
2-N-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming)
Reaction(IUBMB)
N2-(D-1,3-dicarboxypropyl)-L-arginine + NADP+ + H2O = L-arginine + 2-oxoglutarate + NADPH + H+ [RN:R00563]
Reaction(KEGG)
R00563
Substrate
N2-(D-1,3-dicarboxypropyl)-L-arginine [CPD:C01682];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
L-arginine [CPD:C00062];
2-oxoglutarate [CPD:C00026];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
In the reverse direction, forms D-nopaline from L-arginine and D-ornaline from L-ornithine.
History
EC 1.5.1.19 created 1984
Pathway
ec00330  Arginine and proline metabolism
ec01100  Metabolic pathways
Orthology
K00296  D-nopaline dehydrogenase
Genes
ATUAtu6015(nos)
AVIAvi_8195(nos)
ALFCFBP5473_23905
NENNCHU2750_55510(nos)
Reference
1  [PMID:476084]
  Authors
Kemp JD, Sutton DW, Hack E.
  Title
Purification and characterization of the crown gall specific enzyme nopaline synthase.
  Journal
Biochemistry 18:3755-60 (1979)
DOI:10.1021/bi00584a017
Other DBs
ExplorEnz - The Enzyme Database: 1.5.1.19
IUBMB Enzyme Nomenclature: 1.5.1.19
ExPASy - ENZYME nomenclature database: 1.5.1.19
BRENDA, the Enzyme Database: 1.5.1.19
CAS: 64763-57-1
LinkDB

KEGG   REACTION: R00563
Entry
R00563                      Reaction                               
Name
N2-(D-1,3-dicarboxypropyl)-L-arginine:NADP+ oxidoreductase (L-arginine-forming);
D-nopaline:NADP+ oxidoreductase (L-arginine-forming)
Definition
Nopaline + NADP+ + H2O <=> L-Arginine + 2-Oxoglutarate + NADPH + H+
Equation
Reaction class
RC00001  C00005_C00006
RC00283  C00062_C01682
RC01532  C00026_C01682
Enzyme
Pathway
rn00330  Arginine and proline metabolism
rn01100  Metabolic pathways
Orthology
K00296  D-nopaline dehydrogenase [EC:1.5.1.19]
Other DBs
RHEA: 19640
LinkDB

KEGG   REACTION: R12706
Entry
R12706                      Reaction                               
Definition
Nopaline + H2O + Acceptor <=> L-Arginine + 2-Oxoglutarate + Reduced acceptor
Equation
Comment
nopaline oxidase
Reaction class
RC00283  C00062_C01682
Pathway
rn00330  Arginine and proline metabolism
rn01100  Metabolic pathways
Orthology
K24952  nopaline oxidase subunit A
K24953  nopaline oxidase subunit B
LinkDB

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