KEGG ORTHOLOGY: K10150

ORTHOLOGY: K10150

Entry

K10150                      KO

Symbol

cysO

Name

cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase [EC:2.5.1.47 2.5.1.65 4.2.1.22]

Pathway

map00260

Glycine, serine and threonine metabolism

map00270

Cysteine and methionine metabolism

map00670

One carbon pool by folate

map00920

Sulfur metabolism

map01100

Metabolic pathways

map01110

Biosynthesis of secondary metabolites

map01120

Microbial metabolism in diverse environments

map01230

Biosynthesis of amino acids

Module

M00035

Methionine degradation

M00338

Cysteine biosynthesis, homocysteine + serine => cysteine

Reaction

R00891

L-serine hydro-lyase (adding hydrogen sulfide, L-cysteine-forming)

R00897

O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

R01290

L-serine hydro-lyase (adding homocysteine; L-cystathionine-forming)

R03601

O3-acetyl-L-serine acetate-lyase (adding hydrogen sulfide)

R04859

O3-acetyl-L-serine:thiosulfate 2-amino-2-carboxyethyltransferase (reducing, L-cysteine-forming)

R04942

L-serine hydro-lyase (adding homocysteine)

R07274

O-phospho-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Brite

KEGG Orthology (KO) [BR:ko00001]
09100 Metabolism
  09102 Energy metabolism
   00920 Sulfur metabolism
    K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
  09105 Amino acid metabolism
   00260 Glycine, serine and threonine metabolism
    K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
   00270 Cysteine and methionine metabolism
    K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
  09108 Metabolism of cofactors and vitamins
   00670 One carbon pool by folate
    K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
Enzymes [BR:ko01000]
2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.47  cysteine synthase
     K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
    2.5.1.65  O-phosphoserine sulfhydrylase
     K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase
4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.22  cystathionine beta-synthase
     K10150  cysO; cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase

Other DBs

GO:	0004124 0033847 0004122

Genes

APE:	APE_1586(cysK)

ACJ:	ACAM_0997(cysK)

DFD:	Desfe_0630

DMU:	Desmu_0644

TAG:

Tagg_1314

IAG:

Igag_1783

THG:	TCELL_0171

HBU:

Hbut_1585

PFM:	Pyrfu_1108

PDL:	Pyrde_0768

PABI:

PABY_05110

PIS:

Pisl_1761

TPE:

Tpen_1605

THB:	N186_00700

TCB:	TCARB_1222

ASC:

ASAC_0793

ACIA:

SE86_05800

CLG:	Calag_0796

CCAI:

NAS2_0810

» show all

Reference

PMID:12644499

Authors

Mino K, Ishikawa K

Title

Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003

Sequence

[ape:APE_1586]

LinkDB

ENZYME: 2.5.1.65

Entry

EC 2.5.1.65                 Enzyme

Name

O-phosphoserine sulfhydrylase;
O-phosphoserine(thiol)-lyase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

O-phospho-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Reaction(IUBMB)

O-phospho-L-serine + hydrogen sulfide = L-cysteine + phosphate [RN:R07274]

Reaction(KEGG)

R07274;
(other) R00897

Substrate

O-phospho-L-serine [CPD:C01005];
hydrogen sulfide [CPD:C00283]

Product

L-cysteine [CPD:C00097];
phosphate [CPD:C00009]

Comment

A pyridoxal-phosphate protein. The enzyme from Aeropyrum pernix acts on both O-phospho-L-serine and O3-acetyl-L-serine, in contrast with EC 2.5.1.47, cysteine synthase, which acts only on O3-acetyl-L-serine.

History

EC 2.5.1.65 created 2004

Pathway

ec00270

Cysteine and methionine metabolism

ec00920

Sulfur metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

ec01120

Microbial metabolism in diverse environments

Orthology

K10150

cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase

Genes

APE:	APE_1586(cysK)

ACJ:	ACAM_0997(cysK)

DFD:	Desfe_0630

DMU:	Desmu_0644

TAG:

Tagg_1314

IAG:

Igag_1783

THG:	TCELL_0171

HBU:

Hbut_1585

PFM:	Pyrfu_1108

PDL:	Pyrde_0768

PABI:

PABY_05110

PIS:

Pisl_1761

TPE:

Tpen_1605

THB:	N186_00700

TCB:	TCARB_1222

ASC:

ASAC_0793

ACIA:

SE86_05800

CLG:	Calag_0796

CCAI:

NAS2_0810

» show all

Reference

1 [PMID:12965218]

Authors

Mino K, Ishikawa K.

Title

A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

FEBS Lett 551:133-8 (2003)
DOI:10.1016/S0014-5793(03)00913-X

Reference

2 [PMID:12644499]

Authors

Mino K, Ishikawa K

Title

Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003

Sequence

[ape:APE_1586]

Reference

3 [PMID:12554945]

Authors

Mino K, Oda Y, Ataka M, Ishikawa K.

Title

Crystallization and preliminary X-ray diffraction analysis of O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

Acta Crystallogr D Biol Crystallogr 59:338-40 (2003)
DOI:10.1107/S0907444902017900

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.65

IUBMB Enzyme Nomenclature:

2.5.1.65

ExPASy - ENZYME nomenclature database:

2.5.1.65

BRENDA, the Enzyme Database:

2.5.1.65

CAS:	1071505-11-7

LinkDB

REACTION: R00897

Entry

R00897                      Reaction

Name

O3-acetyl-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase;
O3-acetyl-L-serine acetate-lyase (adding hydrogen sulfide)

Definition

O-Acetyl-L-serine + Hydrogen sulfide <=> L-Cysteine + Acetate

Equation

C00979 + C00283 <=> C00097 + C00033

Reaction class

RC00020

C00033_C00979

RC02814

C00097_C00283

RC02821

C00097_C00979

Enzyme

2.5.1.47 2.5.1.65

Pathway

rn00270

Cysteine and methionine metabolism

rn00920

Sulfur metabolism

rn01100

Metabolic pathways

rn01110

Biosynthesis of secondary metabolites

rn01120

Microbial metabolism in diverse environments

rn01200

Carbon metabolism

rn01230

Biosynthesis of amino acids

rn01320

Sulfur cycle

Module

M00021

Cysteine biosynthesis, serine => cysteine

Brite

Enzymatic reactions [BR:br08201]
2. Transferase reactions
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.47
     R00897  O-Acetyl-L-serine + Hydrogen sulfide <=> L-Cysteine + Acetate
    2.5.1.65
     R00897  O-Acetyl-L-serine + Hydrogen sulfide <=> L-Cysteine + Acetate

Orthology

K01738

cysteine synthase [EC:2.5.1.47]

K10150

cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase [EC:2.5.1.47 2.5.1.65 4.2.1.22]

K13034

L-3-cyanoalanine synthase/ cysteine synthase [EC:2.5.1.47 4.4.1.9]

K17069

O-acetylhomoserine/O-acetylserine sulfhydrylase [EC:2.5.1.49 2.5.1.47]

Other DBs

RHEA:

14832

LinkDB

DBGET integrated database retrieval system