KEGG   PATHWAY: ecz00270
Entry
ecz00270                    Pathway                                
Name
Cysteine and methionine metabolism - Escherichia coli O45:K1:H7 S88 (ExPEC)
Description
Cysteine and methionine are sulfur-containing amino acids. Cysteine is synthesized from serine through different pathways in different organism groups. In bacteria and plants, cysteine is converted from serine (via acetylserine) by transfer of hydrogen sulfide [MD:M00021]. In animals, methionine-derived homocysteine is used as sulfur source and its condensation product with serine (cystathionine) is converted to cysteine [MD:M00338]. Cysteine is metabolized to pyruvate in multiple routes. Methionine is an essential amino acid, which animals cannot synthesize. In bacteria and plants, methionine is synthesized from aspartate [MD:M00017]. S-Adenosylmethionine (SAM), synthesized from methionine and ATP, is a methyl group donor in many important transfer reactions including DNA methylation for regulation of gene expression. SAM may also be used to regenerate methionine in the methionine salvage pathway [MD:M00034].
Class
Metabolism; Amino acid metabolism
Pathway map
ecz00270  Cysteine and methionine metabolism
ecz00270

Module
ecz_M00017  Methionine biosynthesis, aspartate => homoserine => methionine [PATH:ecz00270]
ecz_M00021  Cysteine biosynthesis, serine => cysteine [PATH:ecz00270]
Other DBs
GO: 0006534 0006555
Organism
Escherichia coli O45:K1:H7 S88 (ExPEC) [GN:ecz]
Gene
ECS88_4024  cysE; serine acetyltransferase [KO:K00640] [EC:2.3.1.30]
ECS88_2131  wbvC; putative O-acetyltransferase [KO:K00640] [EC:2.3.1.30]
ECS88_2604  cysK; cysteine synthase A, O-acetylserine sulfhydrolase A subunit [KO:K01738] [EC:2.5.1.47]
ECS88_3388  metC; cystathionine beta-lyase, PLP-dependent [KO:K01760] [EC:4.4.1.13]
ECS88_1669  malY; bifunctional beta-cystathionase, PLP-dependent and regulator of maltose regulon [KO:K14155] [EC:4.4.1.13]
ECS88_3109  putative aminotransferase [KO:K14155] [EC:4.4.1.13]
ECS88_4483  metH; homocysteine-N5-methyltetrahydrofolate transmethylase, B12-dependent [KO:K00548] [EC:2.1.1.13]
ECS88_4257  metE; 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase [KO:K00549] [EC:2.1.1.14]
ECS88_3224  metK; methionine adenosyltransferase 1 [KO:K00789] [EC:2.5.1.6]
ECS88_4662  metK; S-adenosylmethionine synthetase (Methionine adenosyltransferase) [KO:K00789] [EC:2.5.1.6]
ECS88_0129  speD; S-adenosylmethionine decarboxylase [KO:K01611] [EC:4.1.1.50]
ECS88_0130  speE; spermidine synthase (putrescine aminopropyltransferase) [KO:K00797] [EC:2.5.1.16]
ECS88_0170  pfs; 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase [KO:K01243] [EC:3.2.2.9]
ECS88_4901  mtnK; Methylthioribose kinase (MTR kinase) [KO:K00899] [EC:2.7.1.100]
ECS88_2781  yfiH; conserved hypothetical protein [KO:K05810] [EC:2.4.2.1 2.4.2.28 3.5.4.4]
ECS88_4902  mtnA; methyl-thioribose-1-phosphate isomerase [KO:K08963] [EC:5.3.1.23]
ECS88_4533  tyrB; tyrosine aminotransferase, tyrosine-repressible, PLP-dependent [KO:K00832] [EC:2.6.1.57]
ECS88_1885  yebR; conserved hypothetical protein [KO:K08968] [EC:1.8.4.14]
ECS88_2014  dcm; DNA cytosine methylase [KO:K00558] [EC:2.1.1.37]
ECS88_2954  luxS; S-ribosylhomocysteinase [KO:K07173] [EC:4.4.1.21]
ECS88_4497  lysC; aspartokinase III [KO:K00928] [EC:2.7.2.4]
ECS88_0002  thrA; fused aspartokinase I; homoserine dehydrogenase I [KO:K12524] [EC:2.7.2.4 1.1.1.3]
ECS88_4391  metL; fused aspartokinase II; homoserine dehydrogenase II [KO:K12525] [EC:2.7.2.4 1.1.1.3]
ECS88_3831  asd; aspartate-semialdehyde dehydrogenase, NAD(P)-binding [KO:K00133] [EC:1.2.1.11]
ECS88_4478  metA; homoserine transsuccinylase [KO:K00651] [EC:2.3.1.46 2.3.1.31]
ECS88_4390  metB; cystathionine gamma-synthase, PLP-dependent [KO:K01739] [EC:2.5.1.48]
ECS88_4193  ilvE; branched-chain amino-acid aminotransferase [KO:K00826] [EC:2.6.1.42]
ECS88_2955  gshA; gamma-glutamate-cysteine ligase [KO:K01919] [EC:6.3.2.2]
ECS88_3229  gshB; glutathione synthetase [KO:K01920] [EC:6.3.2.3]
ECS88_0956  aspC; aspartate aminotransferase, PLP-dependent [KO:K00813] [EC:2.6.1.1]
ECS88_2697  sseA; 3-mercaptopyruvate sulfurtransferase [KO:K01011] [EC:2.8.1.1 2.8.1.2]
ECS88_4540  Putative malate/L-lactate dehydrogenases [KO:K00016] [EC:1.1.1.27]
ECS88_3137  ygeA; putative racemase [KO:K25316] [EC:5.1.1.10]
ECS88_1973  yedO; D-cysteine desulfhydrase, PLP-dependent [KO:K05396] [EC:4.4.1.15]
ECS88_3612  mdh; malate dehydrogenase, NAD(P)-binding [KO:K00024] [EC:1.1.1.37]
ECS88_3066  sdaB; L-serine deaminase II [KO:K01752] [EC:4.3.1.17]
ECS88_3505  tdcG; L-serine dehydratase 3 [KO:K01752] [EC:4.3.1.17]
ECS88_1866  sdaA; L-serine deaminase I [KO:K01752] [EC:4.3.1.17]
ECS88_2610  cysM; cysteine synthase B (O-acetylserine sulfhydrolase B) [KO:K12339] [EC:2.5.1.144]
ECS88_3192  serA; D-3-phosphoglycerate dehydrogenase [KO:K00058] [EC:1.1.1.95 1.1.1.399]
ECS88_3107  2-hydroxyacid dehydrogenase [KO:K00058] [EC:1.1.1.95 1.1.1.399]
ECS88_0935  serC; 3-phosphoserine/phosphohydroxythreonine aminotransferase [KO:K00831] [EC:2.6.1.52]
Compound
C00019  S-Adenosyl-L-methionine
C00021  S-Adenosyl-L-homocysteine
C00022  Pyruvate
C00041  L-Alanine
C00049  L-Aspartate
C00051  Glutathione
C00059  Sulfate
C00065  L-Serine
C00073  L-Methionine
C00094  Sulfite
C00097  L-Cysteine
C00109  2-Oxobutanoate
C00155  L-Homocysteine
C00170  5'-Methylthioadenosine
C00197  3-Phospho-D-glycerate
C00263  L-Homoserine
C00283  Hydrogen sulfide
C00409  Methanethiol
C00441  L-Aspartate 4-semialdehyde
C00491  L-Cystine
C00506  L-Cysteate
C00606  3-Sulfino-L-alanine
C00793  D-Cysteine
C00957  Mercaptopyruvate
C00979  O-Acetyl-L-serine
C01005  O-Phospho-L-serine
C01077  O-Acetyl-L-homoserine
C01118  O-Succinyl-L-homoserine
C01137  S-Adenosylmethioninamine
C01180  4-Methylthio-2-oxobutanoic acid
C01234  1-Aminocyclopropane-1-carboxylate
C01817  L-Homocystine
C01962  Thiocysteine
C02218  Dehydroalanine
C02291  L-Cystathionine
C02356  (S)-2-Aminobutanoate
C02989  L-Methionine S-oxide
C03082  4-Phospho-L-aspartate
C03089  5-Methylthio-D-ribose
C03145  N-Formylmethionine
C03232  3-Phosphonooxypyruvate
C03431  S-Inosyl-L-homocysteine
C03539  S-Ribosyl-L-homocysteine
C04188  S-Methyl-5-thio-D-ribose 1-phosphate
C04582  S-Methyl-5-thio-D-ribulose 1-phosphate
C05324  Nicotianamine
C05524  Aminoacyl-L-methionine
C05526  S-Glutathionyl-L-cysteine
C05527  3-Sulfinylpyruvate
C05528  3-Sulfopyruvate
C05823  3-Mercaptolactate
C05824  S-Sulfo-L-cysteine
C06547  Ethylene
C08276  3-(Methylthio)propanoate
C09306  Sulfur dioxide
C11437  1-Deoxy-D-xylulose 5-phosphate
C11481  HSO3-
C11499  (S)-3-Sulfolactate
C11537  (2R)-3-Sulfolactate
C15606  1,2-Dihydroxy-5-(methylthio)pent-1-en-3-one
C15650  2,3-Diketo-5-methylthiopentyl-1-phosphate
C15651  2-Hydroxy-3-keto-5-methylthiopentenyl-1-phosphate
C18049  N-Acyl-L-homoserine lactone
C19787  5'-S-Methyl-5'-thioinosine
C21015  gamma-L-Glutamyl-L-2-aminobutyrate
C21016  Ophthalmate
C22359  S-Methyl-1-thio-D-xylulose 5-phosphate
Reference
  Authors
Sekowska A, Denervaud V, Ashida H, Michoud K, Haas D, Yokota A, Danchin A
  Title
Bacterial variations on the methionine salvage pathway.
  Journal
BMC Microbiol 4:9 (2004)
DOI:10.1186/1471-2180-4-9
Reference
  Authors
Ashida H, Saito Y, Kojima C, Kobayashi K, Ogasawara N, Yokota A.
  Title
A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO.
  Journal
Science 302:286-90 (2003)
DOI:10.1126/science.1086997
Reference
  Authors
Sekowska A, Danchin A.
  Title
The methionine salvage pathway in Bacillus subtilis.
  Journal
BMC Microbiol 2:8 (2002)
DOI:10.1186/1471-2180-2-8
Reference
  Authors
Berger BJ, English S, Chan G, Knodel MH.
  Title
Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.
  Journal
J Bacteriol 185:2418-31 (2003)
DOI:10.1128/JB.185.8.2418-2431.2003
Reference
  Authors
Goyer A, Collakova E, Shachar-Hill Y, Hanson AD
  Title
Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway.
  Journal
Plant Cell Physiol 48:232-42 (2007)
DOI:10.1093/pcp/pcl055
Reference
  Authors
Rebeille F, Jabrin S, Bligny R, Loizeau K, Gambonnet B, Van Wilder V, Douce R, Ravanel S
  Title
Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine syntheses.
  Journal
Proc Natl Acad Sci U S A 103:15687-92 (2006)
DOI:10.1073/pnas.0606195103
Reference
  Authors
Pirkov I, Norbeck J, Gustafsson L, Albers E
  Title
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway.
  Journal
FEBS J 275:4111-20 (2008)
DOI:10.1111/j.1742-4658.2008.06552.x
Reference
  Authors
Ashida H, Saito Y, Kojima C, Yokota A
  Title
Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway in Bacillus subtilis.
  Journal
Biosci Biotechnol Biochem 72:959-67 (2008)
DOI:10.1271/bbb.70651
Reference
  Authors
Kitabatake M, So MW, Tumbula DL, Soll D
  Title
Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?
  Journal
J Bacteriol 182:143-5 (2000)
DOI:10.1128/JB.182.1.143-145.2000
Reference
  Authors
Mino K, Ishikawa K
  Title
Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
  Journal
J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003
Reference
  Authors
Tanabe S
  Title
Development of assay methods for endogenous inorganic sulfur compounds and sulfurtransferases and evaluation of the physiological functions of bound sulfur.
  Journal
Yakugaku Zasshi 128:881-900 (2008)
DOI:10.1248/yakushi.128.881
Reference
  Authors
Nishizuka Y, Seyama Y, Ikai A, Ishimura Y, Kawaguchi A (eds).
  Title
[Cellular Functions and Metabolic Maps] (In Japanese)
  Journal
Tokyo Kagaku Dojin (1997)
Reference
  Authors
Gutierrez JA, Crowder T, Rinaldo-Matthis A, Ho MC, Almo SC, Schramm VL
  Title
Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing.
  Journal
Nat Chem Biol 5:251-7 (2009)
DOI:10.1038/nchembio.153
Related
pathway
ecz00010  Glycolysis / Gluconeogenesis
ecz00250  Alanine, aspartate and glutamate metabolism
ecz00260  Glycine, serine and threonine metabolism
ecz00290  Valine, leucine and isoleucine biosynthesis
ecz00430  Taurine and hypotaurine metabolism
ecz00480  Glutathione metabolism
ecz00620  Pyruvate metabolism
ecz00640  Propanoate metabolism
ecz00770  Pantothenate and CoA biosynthesis
ecz00900  Terpenoid backbone biosynthesis
ecz00920  Sulfur metabolism
KO pathway
ko00270   
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