KEGG   PATHWAY: ppuu00270
Entry
ppuu00270                   Pathway                                
Name
Cysteine and methionine metabolism - Pseudomonas sp. UW4
Description
Cysteine and methionine are sulfur-containing amino acids. Cysteine is synthesized from serine through different pathways in different organism groups. In bacteria and plants, cysteine is converted from serine (via acetylserine) by transfer of hydrogen sulfide [MD:M00021]. In animals, methionine-derived homocysteine is used as sulfur source and its condensation product with serine (cystathionine) is converted to cysteine [MD:M00338]. Cysteine is metabolized to pyruvate in multiple routes. Methionine is an essential amino acid, which animals cannot synthesize. In bacteria and plants, methionine is synthesized from aspartate [MD:M00017]. S-Adenosylmethionine (SAM), synthesized from methionine and ATP, is a methyl group donor in many important transfer reactions including DNA methylation for regulation of gene expression. SAM may also be used to regenerate methionine in the methionine salvage pathway [MD:M00034].
Class
Metabolism; Amino acid metabolism
Pathway map
ppuu00270  Cysteine and methionine metabolism
ppuu00270

Module
ppuu_M00017  Methionine biosynthesis, aspartate => homoserine => methionine [PATH:ppuu00270]
ppuu_M00021  Cysteine biosynthesis, serine => cysteine [PATH:ppuu00270]
ppuu_M00034  Methionine salvage pathway [PATH:ppuu00270]
ppuu_M00035  Methionine degradation [PATH:ppuu00270]
ppuu_M00338  Cysteine biosynthesis, homocysteine + serine => cysteine [PATH:ppuu00270]
Other DBs
GO: 0006534 0006555
Organism
Pseudomonas sp. UW4 [GN:ppuu]
Gene
PputUW4_00926  cysE; serine O-acetyltransferase [KO:K00640] [EC:2.3.1.30]
PputUW4_00194  serine O-acetyltransferase [KO:K00640] [EC:2.3.1.30]
PputUW4_01177  cysteine synthase [KO:K01738] [EC:2.5.1.47]
PputUW4_04003  cysK; cysteine synthase A [KO:K01738] [EC:2.5.1.47]
PputUW4_03162  cystathionine gamma-lyase [KO:K01758] [EC:4.4.1.1]
PputUW4_02555  aminotransferase [KO:K14155] [EC:4.4.1.13]
PputUW4_03161  cystathionine beta-synthase [KO:K01697] [EC:4.2.1.22]
PputUW4_02354  metH; B12-dependent methionine synthase [KO:K00548] [EC:2.1.1.13]
PputUW4_02038  metE; 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase [KO:K00549] [EC:2.1.1.14]
PputUW4_05068  metK; S-adenosylmethionine synthetase [KO:K00789] [EC:2.5.1.6]
PputUW4_04913  speD; S-adenosylmethionine decarboxylase [KO:K01611] [EC:4.1.1.50]
PputUW4_03691  speE; spermidine synthase [KO:K00797] [EC:2.5.1.16]
PputUW4_02826  spermine/spermidine synthase family protein [KO:K00797] [EC:2.5.1.16]
PputUW4_04652  hypothetical protein [KO:K05810] [EC:2.4.2.1 2.4.2.28 3.5.4.4]
PputUW4_01356  N-ethylammeline chlorohydrolase [KO:K12960] [EC:3.5.4.31 3.5.4.28]
PputUW4_03513  mtnP; 5'-methylthioadenosine phosphorylase [KO:K19696] [EC:2.4.2.44]
PputUW4_01357  mtnA; methylthioribose-1-phosphate isomerase [KO:K08963] [EC:5.3.1.23]
PputUW4_03699  mtnB; methylthioribulose-1-phosphate dehydratase [KO:K08964] [EC:4.2.1.109]
PputUW4_03697  mtnC; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase [KO:K09880] [EC:3.1.3.77]
PputUW4_03698  mtnD; acireductone dioxygenase, ARD [KO:K08967] [EC:1.13.11.53 1.13.11.54]
PputUW4_03469  aromatic amino acid aminotransferase [KO:K00832] [EC:2.6.1.57]
PputUW4_01447  GAF domain-containing protein [KO:K08968] [EC:1.8.4.14]
PputUW4_04188  cytosine-specific methyltransferase [KO:K00558] [EC:2.1.1.37]
PputUW4_05078  ahcY; S-adenosyl-L-homocysteine hydrolase [KO:K01251] [EC:3.13.2.1]
PputUW4_04154  acdS; 1-aminocyclopropane-1-carboxylate deaminase [KO:K01505] [EC:3.5.99.7]
PputUW4_01743  D-cysteine desulfhydrase family protein [KO:K01505] [EC:3.5.99.7]
PputUW4_04164  aspartate kinase [KO:K00928] [EC:2.7.2.4]
PputUW4_03447  asd; aspartate-semialdehyde dehydrogenase [KO:K00133] [EC:1.2.1.11]
PputUW4_04427  homoserine dehydrogenase [KO:K00003] [EC:1.1.1.3]
PputUW4_02644  homoserine dehydrogenase [KO:K00003] [EC:1.1.1.3]
PputUW4_05120  metX; homoserine O-acetyltransferase [KO:K00641] [EC:2.3.1.31 2.3.1.46]
PputUW4_02649  cystathionine gamma-synthase [KO:K01739] [EC:2.5.1.48]
PputUW4_03436  metZ; O-succinylhomoserine sulfhydrylase [KO:K10764] [EC:2.5.1.-]
PputUW4_00866  ilvE1; branched-chain amino acid aminotransferase [KO:K00826] [EC:2.6.1.42]
PputUW4_02966  ilvE2; branched-chain amino acid aminotransferase [KO:K00826] [EC:2.6.1.42]
PputUW4_00201  gshA; glutamate--cysteine ligase [KO:K01919] [EC:6.3.2.2]
PputUW4_05106  gshB; glutathione synthetase [KO:K01920] [EC:6.3.2.3]
PputUW4_03334  aspartate aminotransferase A [KO:K00812] [EC:2.6.1.1]
PputUW4_03915  aromatic amino acid aminotransferase [KO:K00813] [EC:2.6.1.1]
PputUW4_05141  3-mercaptopyruvate sulfurtransferase [KO:K01011] [EC:2.8.1.1 2.8.1.2]
PputUW4_00436  rhdA; thiosulfate sulfurtransferase [KO:K01011] [EC:2.8.1.1 2.8.1.2]
PputUW4_00193  dcyD; D-cysteine desulfhydrase [KO:K05396] [EC:4.4.1.15]
PputUW4_00833  sdaA1; L-serine dehydratase 1 [KO:K01752] [EC:4.3.1.17]
PputUW4_05041  sdaA3; L-serine dehydratase [KO:K01752] [EC:4.3.1.17]
PputUW4_04282  sdaA2; L-serine ammonia-lyase [KO:K01752] [EC:4.3.1.17]
PputUW4_01924  pyridoxal-5'-phosphate-dependent protein beta subunit [KO:K17989] [EC:4.3.1.17 4.3.1.19]
PputUW4_04107  cysM; cysteine synthase B [KO:K12339] [EC:2.5.1.144]
PputUW4_05182  serA2; D-3-phosphoglycerate dehydrogenase [KO:K00058] [EC:1.1.1.95 1.1.1.399]
PputUW4_01359  serC; phosphoserine aminotransferase [KO:K00831] [EC:2.6.1.52]
Compound
C00019  S-Adenosyl-L-methionine
C00021  S-Adenosyl-L-homocysteine
C00022  Pyruvate
C00041  L-Alanine
C00049  L-Aspartate
C00051  Glutathione
C00059  Sulfate
C00065  L-Serine
C00073  L-Methionine
C00094  Sulfite
C00097  L-Cysteine
C00109  2-Oxobutanoate
C00155  L-Homocysteine
C00170  5'-Methylthioadenosine
C00197  3-Phospho-D-glycerate
C00263  L-Homoserine
C00283  Hydrogen sulfide
C00409  Methanethiol
C00441  L-Aspartate 4-semialdehyde
C00491  L-Cystine
C00506  L-Cysteate
C00606  3-Sulfino-L-alanine
C00793  D-Cysteine
C00957  Mercaptopyruvate
C00979  O-Acetyl-L-serine
C01005  O-Phospho-L-serine
C01077  O-Acetyl-L-homoserine
C01118  O-Succinyl-L-homoserine
C01137  S-Adenosylmethioninamine
C01180  4-Methylthio-2-oxobutanoic acid
C01234  1-Aminocyclopropane-1-carboxylate
C01817  L-Homocystine
C01962  Thiocysteine
C02218  Dehydroalanine
C02291  L-Cystathionine
C02356  (S)-2-Aminobutanoate
C02989  L-Methionine S-oxide
C03082  4-Phospho-L-aspartate
C03089  5-Methylthio-D-ribose
C03145  N-Formylmethionine
C03232  3-Phosphonooxypyruvate
C03431  S-Inosyl-L-homocysteine
C03539  S-Ribosyl-L-homocysteine
C04188  S-Methyl-5-thio-D-ribose 1-phosphate
C04582  S-Methyl-5-thio-D-ribulose 1-phosphate
C05324  Nicotianamine
C05524  Aminoacyl-L-methionine
C05526  S-Glutathionyl-L-cysteine
C05527  3-Sulfinylpyruvate
C05528  3-Sulfopyruvate
C05823  3-Mercaptolactate
C05824  S-Sulfo-L-cysteine
C06547  Ethylene
C08276  3-(Methylthio)propanoate
C09306  Sulfur dioxide
C11437  1-Deoxy-D-xylulose 5-phosphate
C11481  HSO3-
C11499  (S)-3-Sulfolactate
C11537  (2R)-3-Sulfolactate
C15606  1,2-Dihydroxy-5-(methylthio)pent-1-en-3-one
C15650  2,3-Diketo-5-methylthiopentyl-1-phosphate
C15651  2-Hydroxy-3-keto-5-methylthiopentenyl-1-phosphate
C18049  N-Acyl-L-homoserine lactone
C19787  5'-S-Methyl-5'-thioinosine
C21015  gamma-L-Glutamyl-L-2-aminobutyrate
C21016  Ophthalmate
C22359  S-Methyl-1-thio-D-xylulose 5-phosphate
Reference
  Authors
Sekowska A, Denervaud V, Ashida H, Michoud K, Haas D, Yokota A, Danchin A
  Title
Bacterial variations on the methionine salvage pathway.
  Journal
BMC Microbiol 4:9 (2004)
DOI:10.1186/1471-2180-4-9
Reference
  Authors
Ashida H, Saito Y, Kojima C, Kobayashi K, Ogasawara N, Yokota A.
  Title
A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO.
  Journal
Science 302:286-90 (2003)
DOI:10.1126/science.1086997
Reference
  Authors
Sekowska A, Danchin A.
  Title
The methionine salvage pathway in Bacillus subtilis.
  Journal
BMC Microbiol 2:8 (2002)
DOI:10.1186/1471-2180-2-8
Reference
  Authors
Berger BJ, English S, Chan G, Knodel MH.
  Title
Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.
  Journal
J Bacteriol 185:2418-31 (2003)
DOI:10.1128/JB.185.8.2418-2431.2003
Reference
  Authors
Goyer A, Collakova E, Shachar-Hill Y, Hanson AD
  Title
Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway.
  Journal
Plant Cell Physiol 48:232-42 (2007)
DOI:10.1093/pcp/pcl055
Reference
  Authors
Rebeille F, Jabrin S, Bligny R, Loizeau K, Gambonnet B, Van Wilder V, Douce R, Ravanel S
  Title
Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine syntheses.
  Journal
Proc Natl Acad Sci U S A 103:15687-92 (2006)
DOI:10.1073/pnas.0606195103
Reference
  Authors
Pirkov I, Norbeck J, Gustafsson L, Albers E
  Title
A complete inventory of all enzymes in the eukaryotic methionine salvage pathway.
  Journal
FEBS J 275:4111-20 (2008)
DOI:10.1111/j.1742-4658.2008.06552.x
Reference
  Authors
Ashida H, Saito Y, Kojima C, Yokota A
  Title
Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway in Bacillus subtilis.
  Journal
Biosci Biotechnol Biochem 72:959-67 (2008)
DOI:10.1271/bbb.70651
Reference
  Authors
Kitabatake M, So MW, Tumbula DL, Soll D
  Title
Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?
  Journal
J Bacteriol 182:143-5 (2000)
DOI:10.1128/JB.182.1.143-145.2000
Reference
  Authors
Mino K, Ishikawa K
  Title
Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
  Journal
J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003
Reference
  Authors
Tanabe S
  Title
Development of assay methods for endogenous inorganic sulfur compounds and sulfurtransferases and evaluation of the physiological functions of bound sulfur.
  Journal
Yakugaku Zasshi 128:881-900 (2008)
DOI:10.1248/yakushi.128.881
Reference
  Authors
Nishizuka Y, Seyama Y, Ikai A, Ishimura Y, Kawaguchi A (eds).
  Title
[Cellular Functions and Metabolic Maps] (In Japanese)
  Journal
Tokyo Kagaku Dojin (1997)
Reference
  Authors
Gutierrez JA, Crowder T, Rinaldo-Matthis A, Ho MC, Almo SC, Schramm VL
  Title
Transition state analogs of 5'-methylthioadenosine nucleosidase disrupt quorum sensing.
  Journal
Nat Chem Biol 5:251-7 (2009)
DOI:10.1038/nchembio.153
Related
pathway
ppuu00010  Glycolysis / Gluconeogenesis
ppuu00250  Alanine, aspartate and glutamate metabolism
ppuu00260  Glycine, serine and threonine metabolism
ppuu00290  Valine, leucine and isoleucine biosynthesis
ppuu00430  Taurine and hypotaurine metabolism
ppuu00480  Glutathione metabolism
ppuu00620  Pyruvate metabolism
ppuu00640  Propanoate metabolism
ppuu00770  Pantothenate and CoA biosynthesis
ppuu00900  Terpenoid backbone biosynthesis
ppuu00920  Sulfur metabolism
KO pathway
ko00270   
LinkDB

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