KEGG   ORTHOLOGY: K05355
Entry
K05355                      KO                                     

Name
hexPS, COQ1
Definition
hexaprenyl-diphosphate synthase [EC:2.5.1.82 2.5.1.83]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K05355  hexPS, COQ1; hexaprenyl-diphosphate synthase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   01006 Prenyltransferases
    K05355  hexPS, COQ1; hexaprenyl-diphosphate synthase
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.82  hexaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]
     K05355  hexPS, COQ1; hexaprenyl-diphosphate synthase
    2.5.1.83  hexaprenyl diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]
     K05355  hexPS, COQ1; hexaprenyl-diphosphate synthase
Prenyltransferases [BR:ko01006]
 Terpene biosynthesis
  Prenyl diphosphate synthase
   K05355  hexPS, COQ1; hexaprenyl-diphosphate synthase
Other DBs
RN: R09245 R09246
COG: COG0142
Genes
QSU: 111992756 112011202
CME: CYME_CMJ246C
SCE: YBR003W(COQ1)
AGO: AGOS_AER004W
ERC: Ecym_5300
KLA: KLLA0_E17183g
KMX: KLMA_60114(COQ1)
LTH: KLTH0G16368g
VPO: Kpol_1036p41
ZRO: ZYRO0A04620g
NCS: NCAS_0I01970(NCAS0I01970)
NDI: NDAI_0A06570(NDAI0A06570)
TPF: TPHA_0A04460(TPHA0A04460)
TBL: TBLA_0F00560(TBLA0F00560)
TDL: TDEL_0D04200(TDEL0D04200)
KAF: KAFR_0F02550(KAFR0F02550)
PIC: PICST_86778(COQ1)
CAL: CAALFM_CR00570WA(CaO19.7478)
SLB: AWJ20_394(COQ1)
NCR: NCU02305
NTE: NEUTE1DRAFT124027(NEUTE1DRAFT_124027)
MGR: MGG_03343
SSCK: SPSK_04068
MAW: MAC_09061
MAJ: MAA_03330
CMT: CCM_00496
BFU: BCIN_02g05540(Bccoq1)
MBE: MBM_07123
ANG: ANI_1_1348094(An11g10370)
ABE: ARB_07252
TVE: TRV_03960
PTE: PTT_13690
ZTR: MYCGRDRAFT_73426(COQ1)
SPO: SPBPJ4664.01(dps1)
CNE: CNI01740
CNB: CNBH1650
ABP: AGABI1DRAFT117445(AGABI1DRAFT_117445) AGABI1DRAFT121951(AGABI1DRAFT_121951)
ABV: AGABI2DRAFT123328(AGABI2DRAFT_123328) AGABI2DRAFT214023(AGABI2DRAFT_214023)
MGL: MGL_1241
MRT: MRET_2902
DFA: DFA_03641(coq1)
PCB: PCHAS_030300(PC000612.04.0)
 » show all
Reference
PMID:2198286
  Authors
Ashby MN, Edwards PA
  Title
Elucidation of the deficiency in two yeast coenzyme Q mutants. Characterization of the structural gene encoding hexaprenyl pyrophosphate synthetase.
  Journal
J Biol Chem 265:13157-64 (1990)
  Sequence
[sce:YBR003W]
LinkDB

KEGG   ORTHOLOGY: K21274
Entry
K21274                      KO                                     

Name
hexs-a
Definition
hexaprenyl-diphosphate synthase small subunit [EC:2.5.1.83]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K21274  hexs-a; hexaprenyl-diphosphate synthase small subunit
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.83  hexaprenyl diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]
     K21274  hexs-a; hexaprenyl-diphosphate synthase small subunit
Other DBs
RN: R09245
GO: 0036423
Genes
MCL: MCCL_1123(hepB1)
AG: BAA25266(hexs-a)
Reference
PMID:9515931
  Authors
Shimizu N, Koyama T, Ogura K
  Title
Molecular cloning, expression, and characterization of the genes encoding the two essential protein components of Micrococcus luteus B-P 26 hexaprenyl diphosphate synthase.
  Journal
J Bacteriol 180:1578-81 (1998)
DOI:10.1128/JB.180.6.1578-1581.1998
  Sequence
LinkDB

KEGG   ORTHOLOGY: K21275
Entry
K21275                      KO                                     

Name
hexs-b
Definition
hexaprenyl-diphosphate synthase large subunit [EC:2.5.1.83]
Pathway
ko00900  Terpenoid backbone biosynthesis
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09109 Metabolism of terpenoids and polyketides
   00900 Terpenoid backbone biosynthesis
    K21275  hexs-b; hexaprenyl-diphosphate synthase large subunit
Enzymes [BR:ko01000]
 2. Transferases
  2.5  Transferring alkyl or aryl groups, other than methyl groups
   2.5.1  Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
    2.5.1.83  hexaprenyl diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]
     K21275  hexs-b; hexaprenyl-diphosphate synthase large subunit
Other DBs
RN: R09245
COG: COG0142
GO: 0036423
Genes
MCL: MCCL_1121(hepB2)
MCAK: MCCS_13080(hexs-b)
MACR: BHM04_03495
AG: BAA25268(hexs-b)
Reference
PMID:9515931
  Authors
Shimizu N, Koyama T, Ogura K
  Title
Molecular cloning, expression, and characterization of the genes encoding the two essential protein components of Micrococcus luteus B-P 26 hexaprenyl diphosphate synthase.
  Journal
J Bacteriol 180:1578-81 (1998)
DOI:10.1128/JB.180.6.1578-1581.1998
  Sequence
LinkDB

KEGG   ENZYME: 2.5.1.83
Entry
EC 2.5.1.83                 Enzyme                                 

Name
hexaprenyl diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific];
HexPS (ambiguous);
hexaprenyl pyrophosphate synthetase (ambiguous);
hexaprenyl diphosphate synthase (ambiguous)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate farnesyltranstransferase (adding 3 isopentenyl units)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + 3 isopentenyl diphosphate = 3 diphosphate + all-trans-hexaprenyl diphosphate [RN:R09245]
Reaction(KEGG)
R09245
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
all-trans-hexaprenyl diphosphate [CPD:C01230]
Comment
The enzyme prefers farnesyl diphosphate to geranylgeranyl diphosphate as an allylic substrate and does not show activity for geranyl diphosphate and dimethylallyl diphosphate [1].
History
EC 2.5.1.83 created 1984 as EC 2.5.1.33, part transferred 2010 to EC 2.5.1.83
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K05355  hexaprenyl-diphosphate synthase
K21274  hexaprenyl-diphosphate synthase small subunit
K21275  hexaprenyl-diphosphate synthase large subunit
Genes
QSU: 111992756 112011202
CME: CYME_CMJ246C
SCE: YBR003W(COQ1)
AGO: AGOS_AER004W
ERC: Ecym_5300
KLA: KLLA0_E17183g
KMX: KLMA_60114(COQ1)
LTH: KLTH0G16368g
VPO: Kpol_1036p41
ZRO: ZYRO0A04620g
NCS: NCAS_0I01970(NCAS0I01970)
NDI: NDAI_0A06570(NDAI0A06570)
TPF: TPHA_0A04460(TPHA0A04460)
TBL: TBLA_0F00560(TBLA0F00560)
TDL: TDEL_0D04200(TDEL0D04200)
KAF: KAFR_0F02550(KAFR0F02550)
PIC: PICST_86778(COQ1)
CAL: CAALFM_CR00570WA(CaO19.7478)
SLB: AWJ20_394(COQ1)
NCR: NCU02305
NTE: NEUTE1DRAFT124027(NEUTE1DRAFT_124027)
MGR: MGG_03343
SSCK: SPSK_04068
MAW: MAC_09061
MAJ: MAA_03330
CMT: CCM_00496
BFU: BCIN_02g05540(Bccoq1)
MBE: MBM_07123
ANG: ANI_1_1348094(An11g10370)
ABE: ARB_07252
TVE: TRV_03960
PTE: PTT_13690
ZTR: MYCGRDRAFT_73426(COQ1)
SPO: SPBPJ4664.01(dps1)
CNE: CNI01740
CNB: CNBH1650
ABP: AGABI1DRAFT117445(AGABI1DRAFT_117445) AGABI1DRAFT121951(AGABI1DRAFT_121951)
ABV: AGABI2DRAFT123328(AGABI2DRAFT_123328) AGABI2DRAFT214023(AGABI2DRAFT_214023)
MGL: MGL_1241
MRT: MRET_2902
DFA: DFA_03641(coq1)
PCB: PCHAS_030300(PC000612.04.0)
MCL: MCCL_1121(hepB2) MCCL_1123(hepB1)
MCAK: MCCS_13080(hexs-b)
 » show all
Reference
1  [PMID:7174655]
  Authors
Fujii H, Koyama T, Ogura K.
  Title
Hexaprenyl pyrophosphate synthetase from Micrococcus luteus B-P 26. Separation of two essential components.
  Journal
J Biol Chem 257:14610-2 (1982)
  Sequence
Reference
2  [PMID:9515931]
  Authors
Shimizu N, Koyama T, Ogura K
  Title
Molecular cloning, expression, and characterization of the genes encoding the two essential protein components of Micrococcus luteus B-P 26 hexaprenyl diphosphate  synthase.
  Journal
J Bacteriol 180:1578-81 (1998)
DOI:10.1128/JB.180.6.1578-1581.1998
  Sequence
Reference
3  [PMID:11514159]
  Authors
Nagaki M, Kimura K, Kimura H, Maki Y, Goto E, Nishino T, Koyama T
  Title
Artificial substrates of medium-chain elongating enzymes, hexaprenyl- and heptaprenyl diphosphate synthases.
  Journal
Bioorg Med Chem Lett 11:2157-9 (2001)
DOI:10.1016/S0960-894X(01)00391-2
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.83
IUBMB Enzyme Nomenclature: 2.5.1.83
ExPASy - ENZYME nomenclature database: 2.5.1.83
BRENDA, the Enzyme Database: 2.5.1.83
LinkDB

KEGG   REACTION: R09245
Entry
R09245                      Reaction                               

Name
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate farnesyltranstransferase (adding 3 isopentenyl units)
Definition
trans,trans-Farnesyl diphosphate + 3 Isopentenyl diphosphate <=> all-trans-Hexaprenyl diphosphate + 3 Diphosphate
Equation
C00448 + 3 C00129 <=> C01230 + 3 C00013
Comment
3-step reaction
see R02061+R07475+R05613
Reaction class
RC00279  C00129_C01230
Enzyme
Pathway
rn00900  Terpenoid backbone biosynthesis
rn01110  Biosynthesis of secondary metabolites
Orthology
K05355  hexaprenyl-diphosphate synthase [EC:2.5.1.82 2.5.1.83]
K21274  hexaprenyl-diphosphate synthase small subunit [EC:2.5.1.83]
K21275  hexaprenyl-diphosphate synthase large subunit [EC:2.5.1.83]
Other DBs
RHEA: 27562
LinkDB

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