KEGG   PATHWAY: csat00510
Entry
csat00510                   Pathway                                
Name
N-Glycan biosynthesis - Camelina sativa (false flax)
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
Pathway map
csat00510  N-Glycan biosynthesis
csat00510

Module
csat_M00055  N-glycan precursor biosynthesis [PATH:csat00510]
csat_M00073  N-glycan precursor trimming [PATH:csat00510]
Other DBs
GO: 0006487
Organism
Camelina sativa (false flax) [GN:csat]
Gene
104766953  polyprenol reductase 2 [KO:K12345] [EC:1.3.1.22 1.3.1.94]
104792193  polyprenol reductase 2-like isoform X1 [KO:K12345] [EC:1.3.1.22 1.3.1.94]
104747449  polyprenol reductase 2-like [KO:K12345] [EC:1.3.1.22 1.3.1.94]
104774124  dolichol kinase EVAN-like [KO:K00902] [EC:2.7.1.108]
104710973  dolichol kinase EVAN [KO:K00902] [EC:2.7.1.108]
104790755  dolichol kinase EVAN-like [KO:K00902] [EC:2.7.1.108]
104785232  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
104782639  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
104793060  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase-like [KO:K01001] [EC:2.7.8.15]
104712390  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
104781626  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase-like [KO:K01001] [EC:2.7.8.15]
104792013  UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase-like [KO:K01001] [EC:2.7.8.15]
104782417  dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
104792787  dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]
104785473  dolichyl-phosphate beta-glucosyltransferase-like [KO:K00729] [EC:2.4.1.117]
104723431  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
104733572  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
104718689  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
104718690  UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]
104723255  UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
104792994  UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
109124633  UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
104776177  probable dolichol-phosphate mannosyltransferase [KO:K00721] [EC:2.4.1.83]
104740755  probable dolichol-phosphate mannosyltransferase [KO:K00721] [EC:2.4.1.83]
104756413  probable dolichol-phosphate mannosyltransferase [KO:K00721] [EC:2.4.1.83]
104713072  dolichol phosphate-mannose biosynthesis regulatory protein [KO:K09658]
109129396  dolichol phosphate-mannose biosynthesis regulatory protein-like [KO:K09658]
104702160  dolichol phosphate-mannose biosynthesis regulatory protein-like [KO:K09658]
104764433  uncharacterized protein LOC104764433 [KO:K09659]
104732156  dolichol-phosphate mannosyltransferase subunit 3-like isoform X1 [KO:K09659]
104758109  dolichol-phosphate mannosyltransferase subunit 3-like [KO:K09659]
104744906  dolichol-phosphate mannosyltransferase subunit 3-like isoform X1 [KO:K09659]
104742319  dolichol-phosphate mannosyltransferase subunit 3-like [KO:K09659]
104777725  dolichol-phosphate mannosyltransferase subunit 3 [KO:K09659]
104775758  UDP-glycosyltransferase TURAN isoform X1 [KO:K03842] [EC:2.4.1.142]
104740340  UDP-glycosyltransferase TURAN-like [KO:K03842] [EC:2.4.1.142]
104755977  UDP-glycosyltransferase TURAN [KO:K03842] [EC:2.4.1.142]
104752305  alpha-1,3/1,6-mannosyltransferase ALG2-like [KO:K03843] [EC:2.4.1.132 2.4.1.257]
104703214  alpha-1,3/1,6-mannosyltransferase ALG2 isoform X1 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
104792874  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase-like [KO:K03844] [EC:2.4.1.131]
104782492  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
104785390  GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
104783359  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase-like isoform X1 [KO:K03845] [EC:2.4.1.258]
104793766  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase-like [KO:K03845] [EC:2.4.1.258]
104784524  dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase isoform X1 [KO:K03845] [EC:2.4.1.258]
104775791  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform X1 [KO:K03846] [EC:2.4.1.259 2.4.1.261]
104740375  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase-like [KO:K03846] [EC:2.4.1.259 2.4.1.261]
104756015  dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase isoform X1 [KO:K03846] [EC:2.4.1.259 2.4.1.261]
104758180  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X2 [KO:K03847] [EC:2.4.1.260]
104773533  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X1 [KO:K03847] [EC:2.4.1.260]
104773734  dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase-like isoform X1 [KO:K03847] [EC:2.4.1.260]
104732997  probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03848] [EC:2.4.1.267]
104719513  probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03848] [EC:2.4.1.267]
104724205  probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase isoform X1 [KO:K03848] [EC:2.4.1.267]
104783018  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
104793415  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
104784871  probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO:K03849] [EC:2.4.1.265]
104708910  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]
104734248  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase-like [KO:K03850] [EC:2.4.1.256]
104768404  dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase-like [KO:K03850] [EC:2.4.1.256]
104777404  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
104757757  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
104770342  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X1 [KO:K07151] [EC:2.4.99.18]
104741985  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B-like [KO:K07151] [EC:2.4.99.18]
104706304  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A isoform X2 [KO:K07151] [EC:2.4.99.18]
104736038  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
104779490  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
104710102  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
104751533  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
104789935  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO:K12666]
109124731  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
104713303  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO:K12666]
104718219  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
104731432  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
104722946  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO:K12667]
104757591  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
104777254  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
104781956  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2-like [KO:K12668]
104741816  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
104792310  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2-like [KO:K12668]
104786002  dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2 [KO:K12668]
104760026  uncharacterized protein LOC104760026 [KO:K12670]
104727367  dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [KO:K12670]
104745505  dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit isoform X1 [KO:K12670]
104714508  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K19478]
104752834  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K19478]
104755407  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3A [KO:K19478]
104739798  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3A [KO:K19478]
104703775  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3B [KO:K19478]
104770285  probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3A [KO:K19478]
104747866  oligosaccharyltransferase complex subunit ostc-like [KO:K26501]
104721873  putative oligosaccharyltransferase complex subunit CG9662 [KO:K26501]
104717210  putative oligosaccharyltransferase complex subunit CG9662 [KO:K26501]
104721894  transmembrane protein 258-like [KO:K26502]
104717226  transmembrane protein 258 [KO:K26502]
104730351  transmembrane protein 258-like [KO:K26502]
104768475  lipid phosphate phosphatase gamma, chloroplastic-like [KO:K07252] [EC:3.6.1.43]
104734321  lipid phosphate phosphatase gamma, chloroplastic-like [KO:K07252] [EC:3.6.1.43]
104708834  lipid phosphate phosphatase gamma, chloroplastic [KO:K07252] [EC:3.6.1.43]
104787756  mannosyl-oligosaccharide glucosidase GCS1 [KO:K01228] [EC:3.2.1.106]
104750548  mannosyl-oligosaccharide glucosidase GCS1-like [KO:K01228] [EC:3.2.1.106]
104741330  alpha-glucosidase 2-like [KO:K01228] [EC:3.2.1.106]
104757041  alpha-glucosidase 2 isoform X1 [KO:K01228] [EC:3.2.1.106]
104701354  mannosyl-oligosaccharide glucosidase GCS1-like [KO:K01228] [EC:3.2.1.106]
104762526  probable glucan 1,3-alpha-glucosidase [KO:K05546] [EC:3.2.1.207]
104741841  probable glucan 1,3-alpha-glucosidase [KO:K05546] [EC:3.2.1.207]
104727012  probable glucan 1,3-alpha-glucosidase isoform X2 [KO:K05546] [EC:3.2.1.207]
104741591  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
104757343  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
104777030  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [KO:K23741] [EC:3.2.1.209]
104758469  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like [KO:K01230] [EC:3.2.1.113]
104786120  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2-like [KO:K01230] [EC:3.2.1.113]
104778068  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1-like [KO:K01230] [EC:3.2.1.113]
104746533  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS2 [KO:K01230] [EC:3.2.1.113]
104742673  mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1 [KO:K01230] [EC:3.2.1.113]
104719899  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like [KO:K00726] [EC:2.4.1.101]
104729134  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like isoform X1 [KO:K00726] [EC:2.4.1.101]
104720738  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1 [KO:K00726] [EC:2.4.1.101]
104719898  alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like [KO:K00726] [EC:2.4.1.101]
104769734  LOW QUALITY PROTEIN: alpha-mannosidase 2-like [KO:K01231] [EC:3.2.1.114]
104735512  alpha-mannosidase 2 isoform X2 [KO:K01231] [EC:3.2.1.114]
104709139  alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
104766544  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like [KO:K00736] [EC:2.4.1.143]
104747180  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase-like [KO:K00736] [EC:2.4.1.143]
104698925  alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00736] [EC:2.4.1.143]
104766619  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104763729  uncharacterized protein LOC104763729 [KO:K00737] [EC:2.4.1.144]
104779999  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104790409  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104793814  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104719414  uncharacterized protein LOC104719414 [KO:K00737] [EC:2.4.1.144]
104787812  uncharacterized protein LOC104787812 isoform X1 [KO:K00737] [EC:2.4.1.144]
104747265  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104769685  uncharacterized protein LOC104769685 isoform X1 [KO:K00737] [EC:2.4.1.144]
104735466  uncharacterized protein LOC104735466 isoform X1 [KO:K00737] [EC:2.4.1.144]
104710595  beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like [KO:K00737] [EC:2.4.1.144]
104755579  uncharacterized protein LOC104755579 [KO:K00737] [EC:2.4.1.144]
104739956  uncharacterized protein LOC104739956 [KO:K00737] [EC:2.4.1.144]
104750600  uncharacterized protein LOC104750600 [KO:K00737] [EC:2.4.1.144]
104701406  uncharacterized protein LOC104701406 [KO:K00737] [EC:2.4.1.144]
104705722  uncharacterized protein LOC104705722 isoform X1 [KO:K00737] [EC:2.4.1.144]
104771942  uncharacterized protein LOC104771942 [KO:K00737] [EC:2.4.1.144]
104773410  uncharacterized protein LOC104773410 [KO:K00737] [EC:2.4.1.144]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
C06081  Polyprenol
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003   
G00004   
G00005   
G00006   
G00007   
G00008   
G00009   
G00010   
G00011   
G00012   
G00013   
G00014   
G00015   
G00016  Core fucose
G00017   
G00018  DS 3
G00019   
G00020   
G00021   
G00022   
G00171   
G10526   
G10595   
G10596   
G10597   
G10598   
G10599   
G10694   
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
PMID:12200473
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208
Reference
PMID:12684507
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
PMID:11278778
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
PMID:15987956
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
PMID:16878994
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
Related
pathway
csat00051  Fructose and mannose metabolism
csat00511  Other glycan degradation
csat00513  Various types of N-glycan biosynthesis
csat00520  Amino sugar and nucleotide sugar metabolism
csat00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
csat00900  Terpenoid backbone biosynthesis
KO pathway
ko00510   
LinkDB

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