KEGG   PATHWAY: emc04530
Entry
emc04530                    Pathway                                
Name
Tight junction - Eublepharis macularius (Leopard gecko)
Description
Tight junctions (TJs) are essential for establishing a selectively permeable barrier to diffusion through the paracellular space between neighboring cells. TJs are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of many different proteins that form large complexes. These are proposed to be involved in junction assembly, barrier regulation, cell polarity, gene transcription, and other pathways.
Class
Cellular Processes; Cellular community - eukaryotes
Pathway map
emc04530  Tight junction
emc04530

Other DBs
GO: 0005923
Organism
Eublepharis macularius (Leopard gecko) [GN:emc]
Gene
129345975  CRB3; protein crumbs homolog 3 isoform X1 [KO:K06090]
129340129  claudin-9-like [KO:K06087]
129340130  claudin-4-like [KO:K06087]
129334761  CLDN23; claudin-23 [KO:K06087]
129340421  CLDN7; claudin-7 [KO:K06087]
129323334  CLDN20; claudin-20 [KO:K06087]
129340906  CLDN2; claudin-2 [KO:K06087]
129341629  CLDN5; claudin-5 [KO:K06087]
129342559  CLDN25; putative claudin-25 [KO:K06087]
129337156  claudin-22-like [KO:K06087]
129325936  CLDN10; claudin-10 isoform X1 [KO:K06087]
129326096  claudin-8-like [KO:K06087]
129332141  CLDN1; claudin-1 [KO:K06087]
129326540  CLDN14; claudin-14 [KO:K06087]
129326696  claudin-8-like [KO:K06087]
129332470  CLDN11; claudin-11 [KO:K06087]
129338426  CLDN15; claudin-15 isoform X1 [KO:K06087]
129332840  CLDN18; claudin-18 [KO:K06087]
129332874  CLDN16; claudin-16 [KO:K06087]
129332929  claudin-19-like [KO:K06087]
129344619  CLDN4; claudin-4 [KO:K06087]
129344766  CLDN19; claudin-19 [KO:K06087]
129344775  CLDN3; claudin-3 [KO:K06087]
129344784  claudin-3-like [KO:K06087]
129338043  CLDN12; claudin-12 [KO:K06087]
129334508  OCLN; occludin [KO:K06088]
129336051  occludin-like [KO:K06088]
129331124  occludin-like [KO:K06088]
129341315  F11R; junctional adhesion molecule A isoform X1 [KO:K06089]
129326504  JAM2; junctional adhesion molecule B isoform X1 [KO:K06735]
129342308  JAM3; junctional adhesion molecule C [KO:K06785]
129327958  BVES; blood vessel epicardial substance isoform X1 [KO:K21108]
129336259  cell division control protein 42 homolog [KO:K04393]
129344557  CDC42; cell division control protein 42 homolog isoform X1 [KO:K04393]
129331148  PARD6B; partitioning defective 6 homolog beta [KO:K06093]
129344278  PARD6A; partitioning defective 6 homolog alpha isoform X1 [KO:K06093]
129333477  PARD6G; partitioning defective 6 homolog gamma [KO:K06093]
129324334  PALS1; protein PALS1 isoform X1 [KO:K06091]
129323622  MPP4; MAGUK p55 subfamily member 4 [KO:K21109]
129330075  TJP3; tight junction protein ZO-3 isoform X1 [KO:K06097]
129329905  PATJ; inaD-like protein [KO:K06092]
129334744  MPDZ; multiple PDZ domain protein [KO:K06095]
129344516  PRKCZ; protein kinase C zeta type [KO:K18952] [EC:2.7.11.13]
129332345  PRKCI; protein kinase C iota type [KO:K06069] [EC:2.7.11.13]
129341026  AMOT; angiomotin [KO:K16819]
129326035  AMOTL1; angiomotin-like protein 1 isoform X1 [KO:K06104]
129332043  AMOTL2; angiomotin-like protein 2 [KO:K06104]
129338451  ARHGAP17; rho GTPase-activating protein 17 isoform X1 [KO:K20638]
129338335  RAC1; ras-related C3 botulinum toxin substrate 1 isoform X1 [KO:K04392]
129341121  NF2; merlin isoform X1 [KO:K16684]
129344957  merlin-like [KO:K16684]
129328935  LLGL2; LLGL scribble cell polarity complex component 2 isoform X1 [KO:K06094]
129338884  LLGL1; lethal(2) giant larvae protein homolog 1 isoform X1 [KO:K06094]
129332146  DLG1; disks large homolog 1 isoform X1 [KO:K12076]
129333330  SCRIB; protein scribble homolog isoform X1 [KO:K16175]
129334521  PPP2CB; serine/threonine-protein phosphatase 2A catalytic subunit beta isoform [KO:K04382] [EC:3.1.3.16]
129329091  serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform isoform X1 [KO:K04382] [EC:3.1.3.16]
129343314  PPP2R1A; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform [KO:K03456]
129328197  PPP2R2B; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform isoform X1 [KO:K04354]
129341968  PPP2R2A; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform isoform X1 [KO:K04354]
129343209  PPP2R2C; protein phosphatase 2, regulatory subunit B, gamma isoform X1 [KO:K04354]
129332095  PPP2R2D; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform isoform X1 [KO:K04354]
129337468  PARD3; partitioning defective 3 homolog [KO:K04237]
129326455  TIAM1; rho guanine nucleotide exchange factor TIAM1 isoform X1 [KO:K05731]
129345417  TJP1; tight junction protein ZO-1 isoform X1 [KO:K05701]
129328227  TJAP1; tight junction-associated protein 1 [KO:K06105]
129326766  DLG2; disks large homolog 2 isoform X1 [KO:K12075]
129341064  DLG3; disks large homolog 3 isoform X1 [KO:K21098]
129345198  NEDD4; E3 ubiquitin-protein ligase NEDD4 isoform X1 [KO:K10591] [EC:2.3.2.26]
129334979  NEDD4L; E3 ubiquitin-protein ligase NEDD4-like isoform X1 [KO:K13305] [EC:2.3.2.26]
129342151  CGN; cingulin [KO:K06102]
129345299  CGNL1; cingulin-like protein 1 isoform X1 [KO:K21110]
129337576  ARHGEF2; rho guanine nucleotide exchange factor 2 isoform X1 [KO:K12791]
129346103  transforming protein RhoA-like [KO:K04513]
129327313  RHOA; transforming protein RhoA [KO:K04513]
129336638  GATA4; transcription factor GATA-4 [KO:K09183]
129334037  MARVEL domain-containing protein 3-like [KO:K21099]
129344108  MARVELD3; MARVEL domain-containing protein 3 isoform X1 [KO:K21099]
129334303  MAP3K1; mitogen-activated protein kinase kinase kinase 1 [KO:K04416] [EC:2.7.11.25]
129328129  MAPK9; mitogen-activated protein kinase 9 [KO:K04440] [EC:2.7.11.24]
129336678  MAPK10; mitogen-activated protein kinase 10 isoform X1 [KO:K04440] [EC:2.7.11.24]
129331890  MAPK8; mitogen-activated protein kinase 8 isoform X1 [KO:K04440] [EC:2.7.11.24]
129329820  JUN; transcription factor Jun [KO:K04448]
129336023  CFTR; cystic fibrosis transmembrane conductance regulator [KO:K05031] [EC:5.6.1.6]
129346578  CDK4; cyclin-dependent kinase 4 [KO:K02089] [EC:2.7.11.22]
129343617  SYMPK; symplekin [KO:K06100]
129342306  PCNA; proliferating cell nuclear antigen [KO:K04802]
129324627  CCND1; G1/S-specific cyclin-D1 isoform X1 [KO:K04503]
129340441  ERBB2; receptor tyrosine-protein kinase erbB-2 [KO:K05083] [EC:2.7.10.1]
129325931  RUNX1; runt-related transcription factor 1 [KO:K08367]
129327401  HSPA4; heat shock 70 kDa protein 4 [KO:K09489]
129328631  NHERF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1 [KO:K13365]
129323498  EZR; ezrin isoform X1 [KO:K08007]
129325689  RDX; radixin isoform X1 [KO:K05762]
129341048  MSN; moesin [KO:K05763]
129332684  PRKCE; protein kinase C epsilon type [KO:K18050] [EC:2.7.11.13]
129345268  actin, cytoplasmic type 5-like [KO:K05692]
129345470  actin-3-like isoform X1 [KO:K05692]
129345681  actin, cytoplasmic type 5 [KO:K05692]
129345837  actin, clone 302-like [KO:K05692]
129332080  actin, clone 302-like isoform X1 [KO:K05692]
129326230  actin, cytoplasmic 1-like [KO:K05692]
129332753  actin, clone 302-like isoform X1 [KO:K05692]
129338420  ACTB; actin, cytoplasmic 1 [KO:K05692]
129327189  ACTG1; actin, cytoplasmic 2 [KO:K05692]
129326985  CACNA1D; voltage-dependent L-type calcium channel subunit alpha-1D isoform X1 [KO:K04851]
129327359  MAP3K5; mitogen-activated protein kinase kinase kinase 5 isoform X1 [KO:K04426] [EC:2.7.11.25]
129327488  MAP2K7; dual specificity mitogen-activated protein kinase kinase 7 isoform X1 [KO:K04431] [EC:2.7.12.2]
129329995  SRC; proto-oncogene tyrosine-protein kinase Src [KO:K05704] [EC:2.7.10.2]
129324192  CTTN; src substrate cortactin isoform X1 [KO:K06106]
129336248  src substrate cortactin-like isoform X1 [KO:K06106]
129334956  ACTR2; actin-related protein 2 [KO:K17260]
129336537  actin-related protein 2-like [KO:K17260]
129324038  ACTR3; actin-related protein 3 [KO:K18584]
129337605  ACTR3B; actin-related protein 3B isoform X1 [KO:K18584]
129340311  ARPC1A; actin-related protein 2/3 complex subunit 1A [KO:K05757]
129338631  ARPC1B; actin-related protein 2/3 complex subunit 1B [KO:K05757]
129324785  ARPC2; actin-related protein 2/3 complex subunit 2 [KO:K05758]
129340869  ARPC3; actin-related protein 2/3 complex subunit 3 [KO:K05756]
129328374  ARPC4; actin-related protein 2/3 complex subunit 4 [KO:K05755]
129342039  ARPC5L; actin-related protein 2/3 complex subunit 5-like protein [KO:K05754]
129331336  ARPC5; actin-related protein 2/3 complex subunit 5 [KO:K05754]
129345692  WHAMM; WASP homolog-associated protein with actin, membranes and microtubules isoform X1 [KO:K20479]
129346319  WAS; LOW QUALITY PROTEIN: actin nucleation-promoting factor WAS [KO:K05747]
129343164  VASP; vasodilator-stimulated phosphoprotein isoform X1 [KO:K06274]
129329909  PRKACB; cAMP-dependent protein kinase catalytic subunit beta isoform X1 [KO:K04345] [EC:2.7.11.11]
129327293  cAMP-dependent protein kinase catalytic subunit alpha [KO:K04345] [EC:2.7.11.11]
129328904  RAB13; ras-related protein Rab-13 [KO:K06109]
129330469  ARHGEF18; rho guanine nucleotide exchange factor 18 [KO:K21066]
129333079  ROCK1; rho-associated protein kinase 1 isoform X1 [KO:K04514] [EC:2.7.11.1]
129323385  ROCK2; rho-associated protein kinase 2 [KO:K17388] [EC:2.7.11.1]
129341209  MYL2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform [KO:K10351]
129337409  EPB41L4B; band 4.1-like protein 4B isoform X1 [KO:K21111]
129330122  STK11; serine/threonine-protein kinase STK11 [KO:K07298] [EC:2.7.11.1]
129334389  PRKAA1; 5'-AMP-activated protein kinase catalytic subunit alpha-1 [KO:K07198] [EC:2.7.11.31]
129331417  PRKAA2; 5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1 [KO:K07198] [EC:2.7.11.31]
129341507  PRKAB1; 5'-AMP-activated protein kinase subunit beta-1 [KO:K07199]
129330974  5'-AMP-activated protein kinase subunit beta-2 [KO:K07199]
129328087  PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1 isoform X1 [KO:K07200]
129323970  PRKAG3; 5'-AMP-activated protein kinase subunit gamma-3 [KO:K07200]
129337557  PRKAG2; 5'-AMP-activated protein kinase subunit gamma-2 isoform X1 [KO:K07200]
129328393  MYH10; myosin-10 isoform X1 [KO:K10352]
129340225  myosin-10-like isoform X1 [KO:K10352]
129335613  MYH9; myosin-9 isoform X1 [KO:K10352]
129338618  MYH11; myosin-11 isoform X1 [KO:K10352]
129329445  myosin light polypeptide 6 isoform X1 [KO:K12751]
129329666  MYL9; myosin regulatory light polypeptide 9 [KO:K12755]
129333140  MYL12B; myosin regulatory light chain 12B isoform X1 [KO:K12757]
129325760  IGSF5; immunoglobulin superfamily member 5 isoform X1 [KO:K06786]
129326881  MAGI1; membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1 [KO:K05631]
129327307  SYNPO; synaptopodin [KO:K21112]
129323722  ACTN1; alpha-actinin-1 isoform X1 [KO:K05699]
129343161  ACTN4; alpha-actinin-4 isoform X1 [KO:K05699]
129340444  MICALL2; MICAL-like protein 2 isoform X1 [KO:K21068]
129329776  RAB8A; ras-related protein Rab-8A isoform X1 [KO:K07901]
129345413  RAB8B; ras-related protein Rab-8B [KO:K07902]
129329441  RAPGEF6; rap guanine nucleotide exchange factor 6 [KO:K08020]
129330698  RAP1A; ras-related protein Rap-1A [KO:K04353]
129337291  ITGB1; integrin beta-1 isoform X1 [KO:K05719]
129325968  AFDN; afadin isoform X1 [KO:K05702]
129334364  TJP2; tight junction protein ZO-2 isoform X1 [KO:K06098]
129336433  RAPGEF2; rap guanine nucleotide exchange factor 2 isoform X1 [KO:K08018]
129341450  RAP2C; ras-related protein Rap-2c [KO:K07839]
129335266  MARVELD2; MARVEL domain-containing protein 2 [KO:K17291]
129329721  OCEL1; occludin/ELL domain-containing protein 1 isoform X1 [KO:K17291]
129345995  tubulin alpha-1C chain [KO:K07374]
129346424  TUBA1A; tubulin alpha-1A chain [KO:K07374]
129346531  tubulin alpha-1B chain [KO:K07374]
129335424  tubulin alpha-4 chain-like [KO:K07374]
129335454  tubulin alpha-3 chain-like [KO:K07374]
129335572  tubulin alpha-3 chain [KO:K07374]
129324308  tubulin alpha-4A chain isoform X1 [KO:K07374]
129335703  tubulin alpha-8 chain [KO:K07374]
129324592  tubulin alpha-1D chain [KO:K07374]
129330515  tubulin alpha-8 chain [KO:K07374]
129332066  tubulin alpha-3 chain [KO:K07374]
Compound
C00076  Calcium cation
Reference
PMID:27353478
  Authors
Zihni C, Mills C, Matter K, Balda MS
  Title
Tight junctions: from simple barriers to multifunctional molecular gates.
  Journal
Nat Rev Mol Cell Biol 17:564-80 (2016)
DOI:10.1038/nrm.2016.80
Reference
PMID:22169974
  Authors
Martin-Belmonte F, Perez-Moreno M
  Title
Epithelial cell polarity, stem cells and cancer.
  Journal
Nat Rev Cancer 12:23-38 (2011)
DOI:10.1038/nrc3169
Reference
PMID:17525755
  Authors
Weber C, Fraemohs L, Dejana E
  Title
The role of junctional adhesion molecules in vascular inflammation.
  Journal
Nat Rev Immunol 7:467-77 (2007)
DOI:10.1038/nri2096
Reference
PMID:15820555
  Authors
Miyoshi J, Takai Y
  Title
Molecular perspective on tight-junction assembly and epithelial polarity.
  Journal
Adv Drug Deliv Rev 57:815-55 (2005)
DOI:10.1016/j.addr.2005.01.008
Reference
PMID:16650994
  Authors
Mertens AE, Pegtel DM, Collard JG
  Title
Tiam1 takes PARt in cell polarity.
  Journal
Trends Cell Biol 16:308-16 (2006)
DOI:10.1016/j.tcb.2006.04.001
Reference
PMID:16771626
  Authors
Shin K, Fogg VC, Margolis B
  Title
Tight junctions and cell polarity.
  Journal
Annu Rev Cell Dev Biol 22:207-35 (2006)
DOI:10.1146/annurev.cellbio.22.010305.104219
Reference
PMID:17182900
  Authors
Kooistra MR, Dube N, Bos JL
  Title
Rap1: a key regulator in cell-cell junction formation.
  Journal
J Cell Sci 120:17-22 (2007)
DOI:10.1242/jcs.03306
Reference
PMID:17950242
  Authors
Gonzalez-Mariscal L, Tapia R, Chamorro D
  Title
Crosstalk of tight junction components with signaling pathways.
  Journal
Biochim Biophys Acta 1778:729-56 (2008)
DOI:10.1016/j.bbamem.2007.08.018
Reference
PMID:18005931
  Authors
Assemat E, Bazellieres E, Pallesi-Pocachard E, Le Bivic A, Massey-Harroche D
  Title
Polarity complex proteins.
  Journal
Biochim Biophys Acta 1778:614-30 (2008)
DOI:10.1016/j.bbamem.2007.08.029
Reference
PMID:18946474
  Authors
Iden S, Collard JG
  Title
Crosstalk between small GTPases and polarity proteins in cell polarization.
  Journal
Nat Rev Mol Cell Biol 9:846-59 (2008)
DOI:10.1038/nrm2521
Reference
PMID:20094051
  Authors
Rosse C, Linch M, Kermorgant S, Cameron AJ, Boeckeler K, Parker PJ
  Title
PKC and the control of localized signal dynamics.
  Journal
Nat Rev Mol Cell Biol 11:103-12 (2010)
DOI:10.1038/nrm2847
Reference
PMID:20134025
  Authors
Terry S, Nie M, Matter K, Balda MS
  Title
Rho signaling and tight junction functions.
  Journal
Physiology (Bethesda) 25:16-26 (2010)
DOI:10.1152/physiol.00034.2009
Reference
PMID:21283798
  Authors
Russ PK, Pino CJ, Williams CS, Bader DM, Haselton FR, Chang MS
  Title
Bves modulates tight junction associated signaling.
  Journal
PLoS One 6:e14563 (2011)
DOI:10.1371/journal.pone.0014563
Reference
PMID:21781017
  Authors
Citi S, Spadaro D, Schneider Y, Stutz J, Pulimeno P
  Title
Regulation of small GTPases at epithelial cell-cell junctions.
  Journal
Mol Membr Biol 28:427-44 (2011)
DOI:10.3109/09687688.2011.603101
Reference
PMID:22896795
  Authors
Lickert H, Van Campenhout CA
  Title
Evolution of the Discs large gene family provides new insights into the establishment of apical epithelial polarity and the etiology of mental retardation.
  Journal
Commun Integr Biol 5:287-90 (2012)
DOI:10.4161/cib.19792
Reference
PMID:22928510
  Authors
Spadaro D, Tapia R, Pulimeno P, Citi S
  Title
The control of gene expression and cell proliferation by the epithelial apical junctional complex.
  Journal
Essays Biochem 53:83-93 (2012)
DOI:10.1042/bse0530083
Reference
PMID:24548561
  Authors
Moleirinho S, Guerrant W, Kissil JL
  Title
The Angiomotins--from discovery to function.
  Journal
FEBS Lett 588:2693-703 (2014)
DOI:10.1016/j.febslet.2014.02.006
Reference
PMID:24651541
  Authors
Rodriguez-Boulan E, Macara IG
  Title
Organization and execution of the epithelial polarity programme.
  Journal
Nat Rev Mol Cell Biol 15:225-42 (2014)
DOI:10.1038/nrm3775
Reference
PMID:25152334
  Authors
Gonzalez-Mariscal L, Dominguez-Calderon A, Raya-Sandino A, Ortega-Olvera JM, Vargas-Sierra O, Martinez-Revollar G
  Title
Tight junctions and the regulation of gene expression.
  Journal
Semin Cell Dev Biol 36:213-23 (2014)
DOI:10.1016/j.semcdb.2014.08.009
Reference
PMID:25469537
  Authors
Mack NA, Georgiou M
  Title
The interdependence of the Rho GTPases and apicobasal cell polarity.
  Journal
Small GTPases 5:10 (2014)
DOI:10.4161/21541248.2014.973768
Reference
PMID:25483301
  Authors
Citi S, Guerrera D, Spadaro D, Shah J
  Title
Epithelial junctions and Rho family GTPases: the zonular signalosome.
  Journal
Small GTPases 5:1-15 (2014)
DOI:10.4161/21541248.2014.973760
Reference
PMID:25757376
  Authors
Zihni C, Terry SJ
  Title
RhoGTPase signalling at epithelial tight junctions: Bridging the GAP between polarity and cancer.
  Journal
Int J Biochem Cell Biol 64:120-5 (2015)
DOI:10.1016/j.biocel.2015.02.020
Reference
PMID:22892070
  Authors
Nakano A, Takashima S
  Title
LKB1 and AMP-activated protein kinase: regulators of cell polarity.
  Journal
Genes Cells 17:737-47 (2012)
DOI:10.1111/j.1365-2443.2012.01629.x
Reference
PMID:12042308
  Authors
Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B
  Title
Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1.
  Journal
J Biol Chem 277:30183-90 (2002)
DOI:10.1074/jbc.M203072200
Reference
PMID:18094055
  Authors
Yamamura R, Nishimura N, Nakatsuji H, Arase S, Sasaki T
  Title
The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
  Journal
Mol Biol Cell 19:971-83 (2008)
DOI:10.1091/mbc.E07-06-0551
Reference
PMID:23885123
  Authors
Monteiro AC, Sumagin R, Rankin CR, Leoni G, Mina MJ, Reiter DM, Stehle T, Dermody TS, Schaefer SA, Hall RA, Nusrat A, Parkos CA
  Title
JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c and regulate epithelial barrier function.
  Journal
Mol Biol Cell 24:2849-60 (2013)
DOI:10.1091/mbc.E13-06-0298
Related
pathway
emc04520  Adherens junction
emc04810  Regulation of actin cytoskeleton
KO pathway
ko04530   
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