KEGG   PATHWAY: npo04530
Entry
npo04530                    Pathway                                
Name
Tight junction - Nyctereutes procyonoides (raccoon dog)
Description
Tight junctions (TJs) are essential for establishing a selectively permeable barrier to diffusion through the paracellular space between neighboring cells. TJs are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of many different proteins that form large complexes. These are proposed to be involved in junction assembly, barrier regulation, cell polarity, gene transcription, and other pathways.
Class
Cellular Processes; Cellular community - eukaryotes
Pathway map
npo04530  Tight junction
npo04530

Other DBs
GO: 0005923
Organism
Nyctereutes procyonoides (raccoon dog) [GN:npo]
Gene
129521508  CRB3; protein crumbs homolog 3 [KO:K06090]
129498277  CLDN12; claudin-12 [KO:K06087]
129507981  CLDN5; claudin-5 [KO:K06087]
129502205  CLDN8; claudin-8 [KO:K06087]
129502206  CLDN17; claudin-17 [KO:K06087]
129502242  CLDN14; claudin-14 [KO:K06087]
129503524  CLDN24; putative claudin-24 [KO:K06087]
129503551  CLDN22; claudin-22 [KO:K06087]
129496924  CLDN25; putative claudin-25 [KO:K06087]
129497353  CLDN7; claudin-7 [KO:K06087]
129498373  CLDN16; claudin-16 [KO:K06087]
129498594  CLDN1; claudin-1 [KO:K06087]
129498684  CLDN11; claudin-11 [KO:K06087]
129499842  CLDN20; claudin-20 [KO:K06087]
129506919  CLDN18; claudin-18 isoform X1 [KO:K06087]
129516015  CLDN23; claudin-23 [KO:K06087]
129516275  CLDN10; claudin-10 isoform X1 [KO:K06087]
129517593  CLDN34; claudin-34 [KO:K06087]
129517699  CLDN2; claudin-2 isoform X1 [KO:K06087]
129519147  CLDN15; claudin-15 isoform X1 [KO:K06087]
129519748  CLDN3; claudin-3 [KO:K06087]
129519929  CLDN4; claudin-4 [KO:K06087]
129520065  CLDN6; claudin-6 [KO:K06087]
129520153  CLDN9; claudin-9 [KO:K06087]
129520738  CLDN19; claudin-19 [KO:K06087]
129513692  OCLN; occludin isoform X1 [KO:K06088]
129515203  F11R; junctional adhesion molecule A isoform X1 [KO:K06089]
129502038  JAM2; junctional adhesion molecule B isoform X1 [KO:K06735]
129497867  JAM3; junctional adhesion molecule C [KO:K06785]
129513087  BVES; blood vessel epicardial substance [KO:K21108]
129520729  cell division control protein 42 homolog [KO:K04393]
129514007  CDC42; cell division control protein 42 homolog isoform X1 [KO:K04393]
129495434  PARD6G; partitioning defective 6 homolog gamma [KO:K06093]
129496141  PARD6B; partitioning defective 6 homolog beta [KO:K06093]
129497767  PARD6A; partitioning defective 6 homolog alpha isoform X1 [KO:K06093]
129514473  partitioning defective 6 homolog gamma-like [KO:K06093]
129496012  PALS1; protein PALS1 isoform X1 [KO:K06091]
129522691  MPP4; MAGUK p55 subfamily member 4 [KO:K21109]
129522425  TJP3; tight junction protein ZO-3 isoform X1 [KO:K06097]
129497135  PATJ; inaD-like protein isoform X1 [KO:K06092]
129494770  MPDZ; multiple PDZ domain protein isoform X1 [KO:K06095]
129497722  PRKCZ; protein kinase C zeta type isoform X1 [KO:K18952] [EC:2.7.11.13]
129498642  PRKCI; protein kinase C iota type isoform X1 [KO:K06069] [EC:2.7.11.13]
129518207  AMOT; angiomotin isoform X1 [KO:K16819]
129506813  AMOTL1; angiomotin-like protein 1 isoform X1 [KO:K06104]
129507061  AMOTL2; angiomotin-like protein 2 isoform X1 [KO:K06104]
129519443  rho GTPase-activating protein 17 isoform X1 [KO:K20638]
129519689  RAC1; ras-related C3 botulinum toxin substrate 1 isoform X1 [KO:K04392]
129507527  NF2; merlin isoform X1 [KO:K16684]
129497125  LLGL1; lethal(2) giant larvae protein homolog 1 [KO:K06094]
129512263  LLGL2; LLGL scribble cell polarity complex component 2 isoform X1 [KO:K06094]
129503650  DLG1; disks large homolog 1 isoform X1 [KO:K12076]
129502594  SCRIB; LOW QUALITY PROTEIN: protein scribble homolog [KO:K16175]
129495162  PPP2CA; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform [KO:K04382] [EC:3.1.3.16]
129515674  PPP2CB; serine/threonine-protein phosphatase 2A catalytic subunit beta isoform [KO:K04382] [EC:3.1.3.16]
129496920  PPP2R1B; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform [KO:K03456]
129500198  PPP2R1A; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform isoform X1 [KO:K03456]
129493701  PPP2R2A; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform [KO:K04354]
129523403  PPP2R2D; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform isoform X1 [KO:K04354]
129510133  PPP2R2C; protein phosphatase 2, regulatory subunit B, gamma isoform X1 [KO:K04354]
129514136  PPP2R2B; serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform isoform X1 [KO:K04354]
129499040  PARD3; partitioning defective 3 homolog isoform X1 [KO:K04237]
129501991  TIAM1; rho guanine nucleotide exchange factor TIAM1 [KO:K05731]
129510301  TJP1; tight junction protein ZO-1 isoform X1 [KO:K05701]
129512929  TJAP1; tight junction-associated protein 1 isoform X1 [KO:K06105]
129506643  DLG2; disks large homolog 2 isoform X1 [KO:K12075]
129518267  DLG3; disks large homolog 3 isoform X1 [KO:K21098]
129506290  NEDD4; E3 ubiquitin-protein ligase NEDD4 isoform X1 [KO:K10591] [EC:2.3.2.26]
129495404  E3 ubiquitin-protein ligase NEDD4-like isoform X1 [KO:K13305] [EC:2.3.2.26]
129514443  E3 ubiquitin-protein ligase NEDD4-like isoform X1 [KO:K13305] [EC:2.3.2.26]
129501480  CGN; cingulin [KO:K06102]
129506209  CGNL1; cingulin-like protein 1 [KO:K21110]
129508858  ARHGEF2; rho guanine nucleotide exchange factor 2 isoform X1 [KO:K12791]
129521354  RHOA; transforming protein RhoA [KO:K04513]
129493670  GATA4; transcription factor GATA-4 [KO:K09183]
129496974  MARVELD3; MARVEL domain-containing protein 3 isoform X1 [KO:K21099]
129513743  MAP3K1; mitogen-activated protein kinase kinase kinase 1 isoform X1 [KO:K04416] [EC:2.7.11.25]
129494670  MAPK9; mitogen-activated protein kinase 9 isoform X1 [KO:K04440] [EC:2.7.11.24]
129501796  MAPK10; mitogen-activated protein kinase 10 isoform X1 [KO:K04440] [EC:2.7.11.24]
129522825  MAPK8; mitogen-activated protein kinase 8 isoform X1 [KO:K04440] [EC:2.7.11.24]
129497811  JUN; transcription factor Jun [KO:K04448]
129515126  T-cell surface glycoprotein CD1a-like [KO:K06448]
129515243  T-cell surface glycoprotein CD1a-like [KO:K06448]
129515244  T-cell surface glycoprotein CD1a-like isoform X1 [KO:K06448]
129515269  T-cell surface glycoprotein CD1e, membrane-associated-like isoform X1 [KO:K06448]
129515271  T-cell surface glycoprotein CD1c-like [KO:K06448]
129515282  T-cell surface glycoprotein CD1a-like isoform X1 [KO:K06448]
129515289  T-cell surface glycoprotein CD1b-like isoform X1 [KO:K06448]
129515355  antigen-presenting glycoprotein CD1d-like [KO:K06448]
129515089  T-cell surface glycoprotein CD1a-like isoform X1 [KO:K06448]
129502846  CFTR; cystic fibrosis transmembrane conductance regulator [KO:K05031] [EC:5.6.1.6]
129511485  CDK4; cyclin-dependent kinase 4 [KO:K02089] [EC:2.7.11.22]
129517117  YBX3; Y-box-binding protein 3 isoform X1 [KO:K06099]
129500187  SYMPK; symplekin [KO:K06100]
129494188  PCNA; proliferating cell nuclear antigen [KO:K04802]
129502968  CCND1; G1/S-specific cyclin-D1 [KO:K04503]
129505762  ERBB2; receptor tyrosine-protein kinase erbB-2 isoform X1 [KO:K05083] [EC:2.7.10.1]
129502033  RUNX1; runt-related transcription factor 1 isoform X1 [KO:K08367]
129494758  HSPA4; heat shock 70 kDa protein 4 [KO:K09489]
129506904  NHERF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1 [KO:K13365]
129500164  EZR; ezrin isoform X1 [KO:K08007]
129496711  RDX; radixin isoform X1 [KO:K05762]
129518616  MSN; moesin [KO:K05763]
129511727  PRKCE; protein kinase C epsilon type isoform X1 [KO:K18050] [EC:2.7.11.13]
129508542  ACTG1; actin, cytoplasmic 2 [KO:K05692]
129519522  ACTB; actin, cytoplasmic 1 [KO:K05692]
129521343  CACNA1D; voltage-dependent L-type calcium channel subunit alpha-1D [KO:K04851]
129509222  MAP3K5; LOW QUALITY PROTEIN: mitogen-activated protein kinase kinase kinase 5 [KO:K04426] [EC:2.7.11.25]
129521885  MAP2K7; dual specificity mitogen-activated protein kinase kinase 7 isoform X1 [KO:K04431] [EC:2.7.12.2]
129494438  SRC; proto-oncogene tyrosine-protein kinase Src [KO:K05704] [EC:2.7.10.2]
129503798  HCLS1; hematopoietic lineage cell-specific protein [KO:K06106]
129500293  CTTN; src substrate cortactin isoform X1 [KO:K06106]
129511728  ACTR2; actin-related protein 2 [KO:K17260]
129503998  ACTR3; actin-related protein 3 [KO:K18584]
129515891  ACTR3B; actin-related protein 3B isoform X1 [KO:K18584]
129519746  ARPC1A; actin-related protein 2/3 complex subunit 1A [KO:K05757]
129520087  ARPC1B; actin-related protein 2/3 complex subunit 1B [KO:K05757]
129522943  ARPC2; actin-related protein 2/3 complex subunit 2 [KO:K05758]
129508098  ARPC3; actin-related protein 2/3 complex subunit 3 [KO:K05756]
129522295  ARPC4; actin-related protein 2/3 complex subunit 4 [KO:K05755]
129506046  ARPC5; actin-related protein 2/3 complex subunit 5 [KO:K05754]
129516354  ARPC5L; actin-related protein 2/3 complex subunit 5-like protein [KO:K05754]
129513049  actin-related protein 2/3 complex subunit 5-like protein [KO:K05754]
129510251  WHAMM; LOW QUALITY PROTEIN: WASP homolog-associated protein with actin, membranes and microtubules [KO:K20479]
129518251  WAS; actin nucleation-promoting factor WAS isoform X1 [KO:K05747]
129500860  VASP; vasodilator-stimulated phosphoprotein isoform X1 [KO:K06274]
129519960  PRKACB; cAMP-dependent protein kinase catalytic subunit beta isoform X1 [KO:K04345] [EC:2.7.11.11]
129521768  PRKACA; cAMP-dependent protein kinase catalytic subunit alpha isoform X1 [KO:K04345] [EC:2.7.11.11]
129508054  ras-related protein Rab-13-like [KO:K06109]
129508721  RAB13; ras-related protein Rab-13 isoform X1 [KO:K06109]
129521669  rho guanine nucleotide exchange factor 18-like isoform X1 [KO:K21066]
129508826  ROCK1; rho-associated protein kinase 1 isoform X1 [KO:K04514] [EC:2.7.11.1]
129510900  ROCK2; rho-associated protein kinase 2 isoform X1 [KO:K17388] [EC:2.7.11.1]
129508394  MYL2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform [KO:K10351]
129494828  EPB41L4B; band 4.1-like protein 4B isoform X1 [KO:K21111]
129521527  STK11; serine/threonine-protein kinase STK11 isoform X1 [KO:K07298] [EC:2.7.11.1]
129497187  PRKAA2; 5'-AMP-activated protein kinase catalytic subunit alpha-2 isoform X1 [KO:K07198] [EC:2.7.11.31]
129509489  PRKAA1; 5'-AMP-activated protein kinase catalytic subunit alpha-1 isoform X1 [KO:K07198] [EC:2.7.11.31]
129508196  PRKAB1; 5'-AMP-activated protein kinase subunit beta-1 [KO:K07199]
129501380  PRKAB2; 5'-AMP-activated protein kinase subunit beta-2 isoform X1 [KO:K07199]
129515690  PRKAG2; 5'-AMP-activated protein kinase subunit gamma-2 isoform X1 [KO:K07200]
129523044  PRKAG3; 5'-AMP-activated protein kinase subunit gamma-3 [KO:K07200]
129517320  PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1 isoform X1 [KO:K07200]
129496805  MYH10; myosin-10 isoform X1 [KO:K10352]
129500427  MYH14; myosin-14 isoform X1 [KO:K10352]
129511292  MYH9; myosin-9 [KO:K10352]
129519313  MYH11; myosin-11 isoform X1 [KO:K10352]
129511638  MYL6B; myosin light chain 6B [KO:K12751]
129511639  MYL6; myosin light polypeptide 6 isoform X1 [KO:K12751]
129493897  MYL9; myosin regulatory light polypeptide 9 [KO:K12755]
129503326  myosin regulatory light polypeptide 9-like isoform X1 [KO:K12755]
129508903  myosin regulatory light polypeptide 9 [KO:K12757]
129509061  myosin regulatory light chain 12B [KO:K12757]
129505623  myosin regulatory light chain 12B-like [KO:K12757]
129501989  IGSF5; immunoglobulin superfamily member 5 isoform X1 [KO:K06786]
129521438  MAGI1; membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 isoform X1 [KO:K05631]
129509658  SYNPO; synaptopodin isoform X1 [KO:K21112]
129495874  ACTN1; alpha-actinin-1 isoform X1 [KO:K05699]
129500278  ACTN4; alpha-actinin-4 [KO:K05699]
129520104  MICALL2; MICAL-like protein 2 isoform X1 [KO:K21068]
129521621  RAB8A; ras-related protein Rab-8A [KO:K07901]
129506424  RAB8B; ras-related protein Rab-8B [KO:K07902]
129494521  RAPGEF6; rap guanine nucleotide exchange factor 6 isoform X1 [KO:K08020]
129501387  RAP1A; ras-related protein Rap-1A [KO:K04353]
129499050  ITGB1; integrin beta-1 [KO:K05719]
129499893  AFDN; afadin isoform X1 [KO:K05702]
129500844  TJP2; LOW QUALITY PROTEIN: tight junction protein ZO-2 [KO:K06098]
129516844  RAPGEF2; rap guanine nucleotide exchange factor 2 isoform X1 [KO:K08018]
129518707  RAP2C; ras-related protein Rap-2c [KO:K07839]
129513585  MARVELD2; MARVEL domain-containing protein 2 isoform X1 [KO:K17291]
129508060  tubulin alpha-3 chain [KO:K07374]
129503404  TUBA8; tubulin alpha-8 chain [KO:K07374]
129505966  tubulin alpha-8 chain [KO:K07374]
129506205  tubulin alpha-1A chain-like [KO:K07374]
129507883  tubulin alpha-3 chain isoform X1 [KO:K07374]
129522834  TUBA4A; tubulin alpha-4A chain [KO:K07374]
129522967  tubulin alpha-1B chain-like [KO:K07374]
129517335  tubulin alpha-1B chain [KO:K07374]
129517394  tubulin alpha-1A chain [KO:K07374]
129517395  tubulin alpha-1C chain [KO:K07374]
129514115  TUBAL3; tubulin alpha chain-like 3 [KO:K07374]
Compound
C00076  Calcium cation
Reference
PMID:27353478
  Authors
Zihni C, Mills C, Matter K, Balda MS
  Title
Tight junctions: from simple barriers to multifunctional molecular gates.
  Journal
Nat Rev Mol Cell Biol 17:564-80 (2016)
DOI:10.1038/nrm.2016.80
Reference
PMID:22169974
  Authors
Martin-Belmonte F, Perez-Moreno M
  Title
Epithelial cell polarity, stem cells and cancer.
  Journal
Nat Rev Cancer 12:23-38 (2011)
DOI:10.1038/nrc3169
Reference
PMID:17525755
  Authors
Weber C, Fraemohs L, Dejana E
  Title
The role of junctional adhesion molecules in vascular inflammation.
  Journal
Nat Rev Immunol 7:467-77 (2007)
DOI:10.1038/nri2096
Reference
PMID:15820555
  Authors
Miyoshi J, Takai Y
  Title
Molecular perspective on tight-junction assembly and epithelial polarity.
  Journal
Adv Drug Deliv Rev 57:815-55 (2005)
DOI:10.1016/j.addr.2005.01.008
Reference
PMID:16650994
  Authors
Mertens AE, Pegtel DM, Collard JG
  Title
Tiam1 takes PARt in cell polarity.
  Journal
Trends Cell Biol 16:308-16 (2006)
DOI:10.1016/j.tcb.2006.04.001
Reference
PMID:16771626
  Authors
Shin K, Fogg VC, Margolis B
  Title
Tight junctions and cell polarity.
  Journal
Annu Rev Cell Dev Biol 22:207-35 (2006)
DOI:10.1146/annurev.cellbio.22.010305.104219
Reference
PMID:17182900
  Authors
Kooistra MR, Dube N, Bos JL
  Title
Rap1: a key regulator in cell-cell junction formation.
  Journal
J Cell Sci 120:17-22 (2007)
DOI:10.1242/jcs.03306
Reference
PMID:17950242
  Authors
Gonzalez-Mariscal L, Tapia R, Chamorro D
  Title
Crosstalk of tight junction components with signaling pathways.
  Journal
Biochim Biophys Acta 1778:729-56 (2008)
DOI:10.1016/j.bbamem.2007.08.018
Reference
PMID:18005931
  Authors
Assemat E, Bazellieres E, Pallesi-Pocachard E, Le Bivic A, Massey-Harroche D
  Title
Polarity complex proteins.
  Journal
Biochim Biophys Acta 1778:614-30 (2008)
DOI:10.1016/j.bbamem.2007.08.029
Reference
PMID:18946474
  Authors
Iden S, Collard JG
  Title
Crosstalk between small GTPases and polarity proteins in cell polarization.
  Journal
Nat Rev Mol Cell Biol 9:846-59 (2008)
DOI:10.1038/nrm2521
Reference
PMID:20094051
  Authors
Rosse C, Linch M, Kermorgant S, Cameron AJ, Boeckeler K, Parker PJ
  Title
PKC and the control of localized signal dynamics.
  Journal
Nat Rev Mol Cell Biol 11:103-12 (2010)
DOI:10.1038/nrm2847
Reference
PMID:20134025
  Authors
Terry S, Nie M, Matter K, Balda MS
  Title
Rho signaling and tight junction functions.
  Journal
Physiology (Bethesda) 25:16-26 (2010)
DOI:10.1152/physiol.00034.2009
Reference
PMID:21283798
  Authors
Russ PK, Pino CJ, Williams CS, Bader DM, Haselton FR, Chang MS
  Title
Bves modulates tight junction associated signaling.
  Journal
PLoS One 6:e14563 (2011)
DOI:10.1371/journal.pone.0014563
Reference
PMID:21781017
  Authors
Citi S, Spadaro D, Schneider Y, Stutz J, Pulimeno P
  Title
Regulation of small GTPases at epithelial cell-cell junctions.
  Journal
Mol Membr Biol 28:427-44 (2011)
DOI:10.3109/09687688.2011.603101
Reference
PMID:22896795
  Authors
Lickert H, Van Campenhout CA
  Title
Evolution of the Discs large gene family provides new insights into the establishment of apical epithelial polarity and the etiology of mental retardation.
  Journal
Commun Integr Biol 5:287-90 (2012)
DOI:10.4161/cib.19792
Reference
PMID:22928510
  Authors
Spadaro D, Tapia R, Pulimeno P, Citi S
  Title
The control of gene expression and cell proliferation by the epithelial apical junctional complex.
  Journal
Essays Biochem 53:83-93 (2012)
DOI:10.1042/bse0530083
Reference
PMID:24548561
  Authors
Moleirinho S, Guerrant W, Kissil JL
  Title
The Angiomotins--from discovery to function.
  Journal
FEBS Lett 588:2693-703 (2014)
DOI:10.1016/j.febslet.2014.02.006
Reference
PMID:24651541
  Authors
Rodriguez-Boulan E, Macara IG
  Title
Organization and execution of the epithelial polarity programme.
  Journal
Nat Rev Mol Cell Biol 15:225-42 (2014)
DOI:10.1038/nrm3775
Reference
PMID:25152334
  Authors
Gonzalez-Mariscal L, Dominguez-Calderon A, Raya-Sandino A, Ortega-Olvera JM, Vargas-Sierra O, Martinez-Revollar G
  Title
Tight junctions and the regulation of gene expression.
  Journal
Semin Cell Dev Biol 36:213-23 (2014)
DOI:10.1016/j.semcdb.2014.08.009
Reference
PMID:25469537
  Authors
Mack NA, Georgiou M
  Title
The interdependence of the Rho GTPases and apicobasal cell polarity.
  Journal
Small GTPases 5:10 (2014)
DOI:10.4161/21541248.2014.973768
Reference
PMID:25483301
  Authors
Citi S, Guerrera D, Spadaro D, Shah J
  Title
Epithelial junctions and Rho family GTPases: the zonular signalosome.
  Journal
Small GTPases 5:1-15 (2014)
DOI:10.4161/21541248.2014.973760
Reference
PMID:25757376
  Authors
Zihni C, Terry SJ
  Title
RhoGTPase signalling at epithelial tight junctions: Bridging the GAP between polarity and cancer.
  Journal
Int J Biochem Cell Biol 64:120-5 (2015)
DOI:10.1016/j.biocel.2015.02.020
Reference
PMID:22892070
  Authors
Nakano A, Takashima S
  Title
LKB1 and AMP-activated protein kinase: regulators of cell polarity.
  Journal
Genes Cells 17:737-47 (2012)
DOI:10.1111/j.1365-2443.2012.01629.x
Reference
PMID:12042308
  Authors
Patrie KM, Drescher AJ, Welihinda A, Mundel P, Margolis B
  Title
Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1.
  Journal
J Biol Chem 277:30183-90 (2002)
DOI:10.1074/jbc.M203072200
Reference
PMID:18094055
  Authors
Yamamura R, Nishimura N, Nakatsuji H, Arase S, Sasaki T
  Title
The interaction of JRAB/MICAL-L2 with Rab8 and Rab13 coordinates the assembly of tight junctions and adherens junctions.
  Journal
Mol Biol Cell 19:971-83 (2008)
DOI:10.1091/mbc.E07-06-0551
Reference
PMID:23885123
  Authors
Monteiro AC, Sumagin R, Rankin CR, Leoni G, Mina MJ, Reiter DM, Stehle T, Dermody TS, Schaefer SA, Hall RA, Nusrat A, Parkos CA
  Title
JAM-A associates with ZO-2, afadin, and PDZ-GEF1 to activate Rap2c and regulate epithelial barrier function.
  Journal
Mol Biol Cell 24:2849-60 (2013)
DOI:10.1091/mbc.E13-06-0298
Related
pathway
npo04520  Adherens junction
npo04810  Regulation of actin cytoskeleton
KO pathway
ko04530   
LinkDB

DBGET integrated database retrieval system