KEGG   ORTHOLOGY: K14534
Entry
K14534                      KO                                     
Symbol
abfD
Name
4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase [EC:4.2.1.120 5.3.3.3]
Pathway
map00650  Butanoate metabolism
map00720  Carbon fixation pathways in prokaryotes
map01100  Metabolic pathways
map01120  Microbial metabolism in diverse environments
map01200  Carbon metabolism
Module
M00374  Dicarboxylate-hydroxybutyrate cycle
M00375  Hydroxypropionate-hydroxybutylate cycle
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09101 Carbohydrate metabolism
   00650 Butanoate metabolism
    K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
  09102 Energy metabolism
   00720 Carbon fixation pathways in prokaryotes
    K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.120  4-hydroxybutanoyl-CoA dehydratase
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
 5. Isomerases
  5.3  Intramolecular oxidoreductases
   5.3.3  Transposing C=C bonds
    5.3.3.3  vinylacetyl-CoA Delta-isomerase
     K14534  abfD; 4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Other DBs
RN: R03031 R10782
COG: COG2368
GO: 0043721 0050393
Genes
BGJ: AWC36_04165
BIZ: HC231_14560
AFUS: EYZ66_08275
NAX: HC341_03525
KAK: Kalk_18475
MGEO: CFI10_04675
PNU: Pnuc_0370
ACHR: C2U31_22605
RHF: EUB48_11505
POS: DT070_18255
RTA: Rta_18760
HYB: Q5W_11860
MPT: Mpe_A2516
LCH: Lcho_2993
METR: BSY238_78
GME: Gmet_2215
DEU: DBW_0157(abfD)
DOL: Dole_2592
DTO: TOL2_C28460(hpaB)
DALK: DSCA_29960(abfD)
DEK: DSLASN_12450(abfD)
SAT: SYN_02445
OCA: OCAR_6245
OCG: OCA5_c17860(hbaB)
OCO: OCA4_c17860(hbaB)
VGO: GJW-30_1_00655(abfD)
RBM: TEF_19955
RID: RIdsm_00769(abfD)
SMAZ: LH19_13280
SINB: SIDU_11465
BACO: OXB_3219
CBE: Cbei_2100
CBZ: Cbs_2100
CBEI: LF65_02412
CKL: CKL_3020(abfD)
CKR: CKR_2668
CPAS: Clopa_4009
CTYK: CTK_C07640(abfD)
OVA: OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
VFA: MM35RIKEN_12830(abfD)
BPRO: PMF13cell1_01502(abfD)
CDF: CD630_23410(abfD)
PDC: CDIF630_02580(abfD)
CDC: CD196_2184(abfD)
CDL: CDR20291_2230(abfD)
PDF: CD630DERM_23410(abfD)
SLP: Slip_0662
DSY: DSY4732
ELM: ELI_0281
CHY: CHY_1314(abfD) CHY_1730
PED: ING2D1G_1210(abfD)
MED: MELS_0342
STED: SPTER_02600(abfD)
AIN: Acin_2034
MID: MIP_03610
SLE: sle_61260(sle_61260)
TCU: Tcur_0955
PGI: PG_0692(abfD)
PGN: PGN_0727
PGT: PGTDC60_1817(abfD)
PCRE: NCTC12858_01801(abfD)
PCAG: NCTC12856_01249(abfD)
TFO: BFO_1484(abfD)
TTK: TST_0398(abfD)
FPL: Ferp_0911
GAH: GAH_01688
FAI: FAD_1294
IHO: Igni_0595
IIS: EYM_00450
SSO: SSO2503(abfD-1) SSO2738(abfD-2)
SAI: Saci_1103 Saci_2143(abfD)
MCN: Mcup_0888
AHO: Ahos_2036
ACIH: HS5_08570
CSTY: KN1_05100
STEP: IC006_0828
PAI: PAE2693
PIS: Pisl_0248
PCL: Pcal_1396
PYR: P186_0718
POG: Pogu_1298
TNE: Tneu_0422
TTN: TTX_1102
TUZ: TUZN_0949
VDI: Vdis_2214
NMR: Nmar_0207
NID: NPIRD3C_0596(abfD)
NIN: NADRNF5_2050(abfD)
NCT: NMSP_1505
NEV: NTE_00645
TAA: NMY3_03315(abfD)
NFN: NFRAN_0724(hcd)
NBV: T478_1248(abfD)
NDV: NDEV_0181(hcd)
LOKI: Lokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)
AG: CAB60035(abfD)
 » show all
Reference
PMID:8344309
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur J Biochem 215:421-9 (1993)
DOI:10.1111/j.1432-1033.1993.tb18049.x
  Sequence
LinkDB

KEGG   ENZYME: 4.2.1.120
Entry
EC 4.2.1.120                Enzyme                                 
Name
4-hydroxybutanoyl-CoA dehydratase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
Sysname
4-hydroxybutanoyl-CoA hydro-lyase
Reaction(IUBMB)
4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O [RN:R10782]
Reaction(KEGG)
R10782
Substrate
4-hydroxybutanoyl-CoA [CPD:C11062]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
H2O [CPD:C00001]
Comment
Contains FAD and a [4Fe-4S] iron-sulfur cluster. The enzyme has been characterized from several microorganisms, including Clostridium kluyveri, where it participates in succinate fermentation [1,2], Clostridium aminobutyricum, where it participates in 4-aminobutyrate degradation [3,4], and Metallosphaera sedula, where it participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [5].
History
EC 4.2.1.120 created 2009
Pathway
ec00650  Butanoate metabolism
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14534  4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase
Genes
BGJAWC36_04165
BIZHC231_14560
AFUSEYZ66_08275
NAXHC341_03525
KAKKalk_18475
MGEOCFI10_04675
PNUPnuc_0370
ACHRC2U31_22605
RHFEUB48_11505
POSDT070_18255
POOF7R28_12325
VPEVarpa_3723
VPDVAPA_1c23470
VAAAX767_00840
VBOCKY39_20160
VAMC4F17_02945
COFFOZ74_14275
RTARta_18760
HYBQ5W_11860
DIHG7047_12160
DRGH9K76_11775
SIMPC6571_16860
MELAC6568_04615
MPTMpe_A2516
METPC1M51_16160
GLCJQN73_05375
LCHLcho_2993
METRBSY238_78
APPCAP2UW1_1984
GMEGmet_2215
DEUDBW_0157(abfD)
DOLDole_2592
DALDalk_0576 Dalk_1215 Dalk_2536 Dalk_2731 Dalk_3391
DTOTOL2_C28460(hpaB)
DWDDSCW_05650(abfD_1) DSCW_20400 DSCW_31010(abfD_2) DSCW_33530 DSCW_58950 DSCW_59050
DALKDSCA_29960(abfD)
DMMdnm_056620
DEKDSLASN_12450(abfD)
SAMYDB32_005276
SATSYN_02445
DTIDesti_0809 Desti_1603 Desti_2004 Desti_3009 Desti_3036
SFUSfum_3141 Sfum_3932
DBRDeba_0626 Deba_1051
DAVDESACE_06790
BRKCWS35_16070 CWS35_35405
BELBE61_32540
BSEPHAP48_0025570
BQBJ4P68_0010490
OCAOCAR_6245
OCGOCA5_c17860(hbaB)
OCOOCA4_c17860(hbaB)
VGOGJW-30_1_00655(abfD)
BOIBLM15_19040
RHZRHPLAN_22730
RBMTEF_19955
PSTGE8M01_28340
RIDRIdsm_00769(abfD)
NOGGKE62_06270
SMAGAN936_12040
SMAZLH19_13280
SPHQBWQ93_08330
RDICMV14_07515
SPHAD3Y57_03405
SJPSJA_C2-03820
SINBSIDU_11465
SPHJBSL82_04790
DEXHWD60_00500
DVNHQ394_03595
BACOOXB_3219
EFFskT53_09470
CBECbei_2100
CBZCbs_2100
CBEILF65_02412
CKLCKL_3020(abfD)
CKRCKR_2668
CLSCXIVA_14640
CPASClopa_4009
CSQCSCA_3616 CSCA_3622
CCKCcar_01580
CTYKCTK_C07640(abfD)
CEUA7L45_11985
CDRKB9W14_01580
CDYF3K33_10480
CFERD4Z93_08285 D4Z93_11490
CCAAKQH81_12640
CRSFQB35_09340
CPRFK7H06_04670 K7H06_04690
FPLAA4U99_00480
CAPREQM14_06625 EQM14_09375 EQM14_10020
OVAOBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)
OBJEIO64_09505
VFAMM35RIKEN_12830(abfD)
BPROPMF13cell1_01502(abfD)
LACYA4V08_31375
ARFAR1Y2_2847 AR1Y2_3067
ACACEYQ97_04915
CDFCD630_23410(abfD)
PDCCDIF630_02580(abfD)
CDCCD196_2184(abfD)
CDLCDR20291_2230(abfD)
PDFCD630DERM_23410(abfD)
SLPSlip_0662
DSYDSY4732
DDHDesde_1768
DMTDESME_04405
DORDesor_0848 Desor_0856 Desor_0867 Desor_2541 Desor_3713
DAIDesaci_1066 Desaci_1680
DMIDesmer_0870 Desmer_3350
PTHPTH_1222 PTH_1770
TFRBR63_00565 BR63_09495 BR63_10065 BR63_10755 BR63_16155 BR63_17815 BR63_17890
FWADCMF_17520 DCMF_19260 DCMF_24550 DCMF_27885
AACXDEACI_0207 DEACI_1870
ELMELI_0281
EMTCPZ25_012290
ELIMB2M23_04455
CMIUB1H56_01400 B1H56_13815
IBUIB211_00283 IB211_01887c
AMIJEQM06_00080 EQM06_05020
ABUTAmi103574_00850
ETMCE91St48_13840(abfD_1) CE91St48_25600(abfD_2) CE91St48_30890 CE91St48_33910(abfD_3)
CHYCHY_1314(abfD) CHY_1730
PEDING2D1G_1210(abfD)
SPOAEQM13_05475
MEDMELS_0342
MHWACT01_02930 ACT01_03000 ACT01_03330 ACT01_05095 ACT01_09475
MEGDKB62_02945 DKB62_07650
STEDSPTER_02600(abfD)
AINAcin_2034
MIDMIP_03610
MCHIAN480_13630
MMALCKJ54_11700
MDXBTO20_21570
ROAPd630_LPD17032
RGOKYT97_22845
GPOGPOL_c49520
GTABCM27_04235
GAMGII34_07415
SLEsle_61260(sle_61260)
SFEUIM697_23390
NANOG5V58_14050
TCUTcur_0955
MCRAID554_30515
VERHUT12_14055
DVCDvina_12895
ERZER308_02395
TRMJO41_12025
TRZGWP43_07785
TMEDDWB79_09475
FNCHMPREF0946_00150
FNTHMPREF0405_01111
FVAFV113G1_21520
FULC4N20_16050
FNFBSQ88_08665
FCII6I83_08420
PGIPG_0692(abfD)
PGNPGN_0727
PGTPGTDC60_1817(abfD)
PAHPoras_0448
PCRENCTC12858_01801(abfD)
PCAGNCTC12856_01249(abfD)
PENDLA319_00265
TFOBFO_1484(abfD)
OSPOdosp_2062
BUYD8S85_11120
BVIRI6J59_04675
RSIRunsl_1400
RUNDR864_15875
SHYRLA303_00675
TTKTST_0398(abfD)
AFUAF_0333 AF_1027
AFGAFULGI_00003320 AFULGI_00011230
ASTAsulf_01733
FPLFerp_0911
GACGACE_0594 GACE_1499
GAHGAH_01688
FACFACI_IFERC01G0375
FAIFAD_1294
IHOIgni_0595
IISEYM_00450
PFMPyrfu_1275
STOSTK_00630 STK_16590
SOHD1869_09350 D1869_14735
SSOSSO2503(abfD-1) SSO2738(abfD-2)
SOLSsol_0312 Ssol_0550
SSOASULA_0305 SULA_0541
SSOLSULB_0307 SULB_0543
SSOFSULC_0305 SULC_0541
SSHIJ5U23_00761 J5U23_01288
SCASSACC_14040 SACC_17730 SACC_24830
SAISaci_1103 Saci_2143(abfD)
SACNSacN8_05365 SacN8_10430
SACRSacRon12I_05355 SacRon12I_10685
SACSSUSAZ_05145 SUSAZ_09780
SISLS215_0322 LS215_2744
SIAM1425_0293 M1425_2585
SIMM1627_0293 M1627_2638
SIDM164_0311 M164_2569
SIYYG5714_0299 YG5714_2751
SINYN1551_0139 YN1551_2902
SIILD85_0299 LD85_2896
SIHSiH_0300 SiH_2522
SIRSiRe_0298 SiRe_2362
SICSiL_0284 SiL_2419
SULABFU36_06625 BFU36_11260
SULEGFS03_00420 GFS03_03030
SULOGFS33_05550 GFS33_08205
SULLEWF20_01055 EWF20_10955
MSEMsed_1220 Msed_1321
MCNMcup_0888
MHKDFR87_12060
MPRUDFR88_00405 DFR88_11715
MTENGWK48_00525 GWK48_10830
MJNMjAS7_2228 MjAS7_2390
AHOAhos_2036
AMANB6F84_01555 B6F84_05320
ABRIDFR85_01140 DFR85_12315
ASULDFR86_01545
AAMBD1866_05205
ACIHHS5_08570
SAZOD1868_09500
CSTYKN1_05100
STEPIC006_0828
PAIPAE2693
PISPisl_0248
PCLPcal_1396
PYRP186_0718
POGPogu_1298
TNETneu_0422
PYWPYWP30_00440
TTNTTX_1102
TUZTUZN_0949
VDIVdis_2214
VMOVMUT_0681 VMUT_2253
CLGCalag_0024
NMRNmar_0207
NIRNSED_00835
NKRNKOR_00855
NIDNPIRD3C_0596(abfD)
NINNADRNF5_2050(abfD)
NIWNisw_03935
NCLC5F47_01015
NOXC5F49_01000
NUEC5F50_01330
NIPNsoK4_07890
NCTNMSP_1505
NICDSQ20_01275
CSYCENSYa_0022
NGANgar_c34390
NVNNVIE_017160
NEVNTE_00645
TAANMY3_03315(abfD)
NFNNFRAN_0724(hcd)
NCVNCAV_0126 NCAV_0127
NBVT478_1248(abfD)
TAHSU86_007675
NIUDSQ19_09180
NDVNDEV_0181(hcd)
BARCAOA65_2031 AOA65_2222
LOKILokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)
 » show all
Reference
1  [PMID:13687513]
  Authors
BARTSCH RG, BARKER HA
  Title
A vinylacetyl isomerase from Clostridium kluyveri.
  Journal
Arch Biochem Biophys 92:122-32 (1961)
DOI:10.1016/0003-9861(61)90226-0
Reference
2  [PMID:8161284]
  Authors
Scherf U, Sohling B, Gottschalk G, Linder D, Buckel W
  Title
Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase.
  Journal
Arch Microbiol 161:239-45 (1994)
DOI:10.1007/bf00248699
  Sequence
[ckl:CKL_3020]
Reference
3  [PMID:8344309]
  Authors
Scherf U, Buckel W
  Title
Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.
  Journal
Eur J Biochem 215:421-9 (1993)
DOI:10.1111/j.1432-1033.1993.tb18049.x
  Sequence
Reference
4  [PMID:8794752]
  Authors
Muh U, Cinkaya I, Albracht SP, Buckel W
  Title
4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction.
  Journal
Biochemistry 35:11710-8 (1996)
DOI:10.1021/bi9601363
Reference
5  [PMID:18079405]
  Authors
Berg IA, Kockelkorn D, Buckel W, Fuchs G
  Title
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
  Journal
Science 318:1782-6 (2007)
DOI:10.1126/science.1149976
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.120
IUBMB Enzyme Nomenclature: 4.2.1.120
ExPASy - ENZYME nomenclature database: 4.2.1.120
BRENDA, the Enzyme Database: 4.2.1.120
LinkDB

KEGG   REACTION: R10782
Entry
R10782                      Reaction                               
Name
4-hydroxybutanoyl-CoA hydro-lyase
Definition
4-Hydroxybutyryl-CoA <=> Crotonoyl-CoA + H2O
Equation
Reaction class
RC03277  C00877_C11062
Enzyme
Pathway
rn00650  Butanoate metabolism
rn00720  Carbon fixation pathways in prokaryotes
rn01100  Metabolic pathways
rn01120  Microbial metabolism in diverse environments
rn01200  Carbon metabolism
Module
M00374  Dicarboxylate-hydroxybutyrate cycle
M00375  Hydroxypropionate-hydroxybutylate cycle
Orthology
K14534  4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase [EC:4.2.1.120 5.3.3.3]
Other DBs
RHEA: 26533
LinkDB

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