KEGG PATHWAY: kpt00270

PATHWAY: kpt00270

Entry

kpt00270                    Pathway

Name

Cysteine and methionine metabolism - Klebsiella pneumoniae subsp. pneumoniae ATCC 43816 KPPR1

Description

Cysteine and methionine are sulfur-containing amino acids. Cysteine is synthesized from serine through different pathways in different organism groups. In bacteria and plants, cysteine is converted from serine (via acetylserine) by transfer of hydrogen sulfide [MD:M00021]. In animals, methionine-derived homocysteine is used as sulfur source and its condensation product with serine (cystathionine) is converted to cysteine [MD:M00338]. Cysteine is metabolized to pyruvate in multiple routes. Methionine is an essential amino acid, which animals cannot synthesize. In bacteria and plants, methionine is synthesized from aspartate [MD:M00017]. S-Adenosylmethionine (SAM), synthesized from methionine and ATP, is a methyl group donor in many important transfer reactions including DNA methylation for regulation of gene expression. SAM may also be used to regenerate methionine in the methionine salvage pathway [MD:M00034].

Class

Metabolism; Amino acid metabolism

Pathway map

kpt00270

Cysteine and methionine metabolism

Module

kpt_M00017

Methionine biosynthesis, aspartate => homoserine => methionine [PATH:kpt00270]

kpt_M00021

Cysteine biosynthesis, serine => cysteine [PATH:kpt00270]

kpt_M00034

Methionine salvage pathway [PATH:kpt00270]

kpt_M00035

Methionine degradation [PATH:kpt00270]

kpt_M00338

Cysteine biosynthesis, homocysteine + serine => cysteine [PATH:kpt00270]

Other DBs

GO:	0006534 0006555

Organism

Klebsiella pneumoniae subsp. pneumoniae ATCC 43816 KPPR1 [GN:kpt]

Gene

VK055_3519

cysE; serine O-acetyltransferase [KO:K00640] [EC:2.3.1.30]

VK055_2143

pyridoxal-phosphate dependent enzyme family protein [KO:K01738] [EC:2.5.1.47]

VK055_4750

cysK; cysteine synthase A [KO:K01738] [EC:2.5.1.47]

VK055_4065

metC3; cystathionine beta-lyase [KO:K01758] [EC:4.4.1.1]

VK055_0773

metC; cystathionine beta-lyase [KO:K01760] [EC:4.4.1.13]

VK055_4046

metC2; cystathionine beta-lyase [KO:K01760] [EC:4.4.1.13]

VK055_0996

aminotransferase class I and II family protein [KO:K14155] [EC:4.4.1.13]

VK055_4066

CBS domain protein [KO:K01697] [EC:4.2.1.22]

VK055_2246

homocysteine S-methyltransferase family protein [KO:K00547] [EC:2.1.1.10]

VK055_3075

metH; methionine synthase [KO:K00548] [EC:2.1.1.13]

VK055_0305

cobalamin-independent synthase, Catalytic domainprotein [KO:K00549] [EC:2.1.1.14]

VK055_3151

metE; 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase [KO:K00549] [EC:2.1.1.14]

VK055_4104

metK; methionine adenosyltransferase [KO:K00789] [EC:2.5.1.6]

VK055_2443

speD; S-adenosylmethionine decarboxylase proenzyme [KO:K01611] [EC:4.1.1.50]

VK055_2442

speE; spermidine synthase [KO:K00797] [EC:2.5.1.16]

VK055_2391

mtnN; MTA/SAH nucleosidase [KO:K01243] [EC:3.2.2.9]

VK055_1908

mtnK; 5-methylthioribose kinase [KO:K00899] [EC:2.7.1.100]

VK055_0346

multi-copper polyphenol oxidoreductase laccase family protein [KO:K05810] [EC:2.4.2.1 2.4.2.28 3.5.4.4]

VK055_4584

hypothetical protein [KO:K05810] [EC:2.4.2.1 2.4.2.28 3.5.4.4]

VK055_1907

mtnA; S-methyl-5-thioribose-1-phosphate isomerase [KO:K08963] [EC:5.3.1.23]

VK055_1899

mtnB; methylthioribulose-1-phosphate dehydratase [KO:K08964] [EC:4.2.1.109]

VK055_1889

mtnC; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase [KO:K09880] [EC:3.1.3.77]

VK055_1890

mtnD; acireductone dioxygenase [KO:K08967] [EC:1.13.11.53 1.13.11.54]

VK055_1071

tyrB; tyrosine aminotransferase [KO:K00832] [EC:2.6.1.57]

VK055_3030

tyrB2; tyrosine aminotransferase [KO:K00832] [EC:2.6.1.57]

VK055_1075

aminotransferase class I and II family protein [KO:K08969] [EC:2.6.1.117]

VK055_0101

yebR; free methionine-(R)-sulfoxide reductase [KO:K08968] [EC:1.8.4.14]

VK055_0016

dcm; cytosine-specific methyltransferase [KO:K00558] [EC:2.1.1.37]

VK055_5150

C-5 cytosine-specific DNA methylase family protein [KO:K00558] [EC:2.1.1.37]

VK055_5058

S-adenosyl-L-homocysteine hydrolase, NAD bindingdomain protein [KO:K01251] [EC:3.13.2.1]

VK055_4498

luxS; S-ribosylhomocysteine lyase [KO:K07173] [EC:4.4.1.21]

VK055_3062

aspartate kinase, monofunctional class [KO:K00928] [EC:2.7.2.4]

VK055_2572

thrA; thrA [KO:K12524] [EC:2.7.2.4 1.1.1.3]

VK055_3241

metL; metL [KO:K12525] [EC:2.7.2.4 1.1.1.3]

VK055_0716

asd; aspartate-semialdehyde dehydrogenase [KO:K00133] [EC:1.2.1.11]

VK055_3672

asd2; aspartate-semialdehyde dehydrogenase [KO:K00133] [EC:1.2.1.11]

VK055_3080

metA; homoserine O-succinyltransferase [KO:K00651] [EC:2.3.1.46 2.3.1.31]

VK055_3243

metB; O-succinylhomoserine (thiol)-lyase [KO:K01739] [EC:2.5.1.48]

VK055_3206

ilvE; branched-chain amino acid aminotransferase [KO:K00826] [EC:2.6.1.42]

VK055_4497

gshA; glutamate--cysteine ligase [KO:K01919] [EC:6.3.2.2]

VK055_4099

gshB; glutathione synthase [KO:K01920] [EC:6.3.2.3]

VK055_1528

aspC; aspC [KO:K00813] [EC:2.6.1.1]

VK055_4649

sseA; 3-mercaptopyruvate sulfurtransferase [KO:K01011] [EC:2.8.1.1 2.8.1.2]

VK055_0858

L-lactate dehydrogenase [KO:K00016] [EC:1.1.1.27]

VK055_4271

putative aspartate/glutamate racemase [KO:K25316] [EC:5.1.1.10]

VK055_0027

dcyD; D-cysteine desulfhydrase monomer [KO:K05396] [EC:4.4.1.15]

VK055_3833

mdh; malate dehydrogenase, NAD-dependent [KO:K00024] [EC:1.1.1.37]

VK055_4374

L-serine ammonia-lyase [KO:K01752] [EC:4.3.1.17]

VK055_0120

L-serine ammonia-lyase [KO:K01752] [EC:4.3.1.17]

VK055_4743

cysM; cysteine synthase B [KO:K12339] [EC:2.5.1.144]

VK055_0864

gyaR; glyoxylate reductase [KO:K00058] [EC:1.1.1.95 1.1.1.399]

VK055_2302

D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain protein [KO:K00058] [EC:1.1.1.95 1.1.1.399]

VK055_1897

S-adenosyl-L-homocysteine hydrolase, NAD bindingdomain protein [KO:K00058] [EC:1.1.1.95 1.1.1.399]

VK055_4135

serA; serA [KO:K00058] [EC:1.1.1.95 1.1.1.399]

VK055_1549

serC; phosphoserine transaminase [KO:K00831] [EC:2.6.1.52]

Compound

C00019

S-Adenosyl-L-methionine

C00021

S-Adenosyl-L-homocysteine

C00022

Pyruvate

C00041

L-Alanine

C00049

L-Aspartate

C00051

Glutathione

C00059

Sulfate

C00065

L-Serine

C00073

L-Methionine

C00094

Sulfite

C00097

L-Cysteine

C00109

2-Oxobutanoate

C00155

L-Homocysteine

C00170

5'-Methylthioadenosine

C00197

3-Phospho-D-glycerate

C00263

L-Homoserine

C00283

Hydrogen sulfide

C00409

Methanethiol

C00441

L-Aspartate 4-semialdehyde

C00491

L-Cystine

C00506

L-Cysteate

C00606

3-Sulfino-L-alanine

C00793

D-Cysteine

C00957

Mercaptopyruvate

C00979

O-Acetyl-L-serine

C01005

O-Phospho-L-serine

C01077

O-Acetyl-L-homoserine

C01118

O-Succinyl-L-homoserine

C01137

S-Adenosylmethioninamine

C01180

4-Methylthio-2-oxobutanoic acid

C01234

1-Aminocyclopropane-1-carboxylate

C01817

L-Homocystine

C01962

Thiocysteine

C02218

Dehydroalanine

C02291

L-Cystathionine

C02356

(S)-2-Aminobutanoate

C02989

L-Methionine S-oxide

C03082

4-Phospho-L-aspartate

C03089

5-Methylthio-D-ribose

C03145

N-Formylmethionine

C03232

3-Phosphonooxypyruvate

C03431

S-Inosyl-L-homocysteine

C03539

S-Ribosyl-L-homocysteine

C04188

S-Methyl-5-thio-D-ribose 1-phosphate

C04582

S-Methyl-5-thio-D-ribulose 1-phosphate

C05324

Nicotianamine

C05524

Aminoacyl-L-methionine

C05526

S-Glutathionyl-L-cysteine

C05527

3-Sulfinylpyruvate

C05528

3-Sulfopyruvate

C05823

3-Mercaptolactate

C05824

S-Sulfo-L-cysteine

C06547

Ethylene

C08276

3-(Methylthio)propanoate

C09306

Sulfur dioxide

C11437

1-Deoxy-D-xylulose 5-phosphate

C11481

HSO3-

C11499

(S)-3-Sulfolactate

C11537

(2R)-3-Sulfolactate

C15606

1,2-Dihydroxy-5-(methylthio)pent-1-en-3-one

C15650

2,3-Diketo-5-methylthiopentyl-1-phosphate

C15651

2-Hydroxy-3-keto-5-methylthiopentenyl-1-phosphate

C18049

N-Acyl-L-homoserine lactone

C19787

5'-S-Methyl-5'-thioinosine

C21015

gamma-L-Glutamyl-L-2-aminobutyrate

C21016

Ophthalmate

C22359

S-Methyl-1-thio-D-xylulose 5-phosphate

Reference

PMID:15102328

Authors

Sekowska A, Denervaud V, Ashida H, Michoud K, Haas D, Yokota A, Danchin A

Title

Bacterial variations on the methionine salvage pathway.

Journal

BMC Microbiol 4:9 (2004)
DOI:10.1186/1471-2180-4-9

Reference

PMID:14551435

Authors

Ashida H, Saito Y, Kojima C, Kobayashi K, Ogasawara N, Yokota A.

Title

A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO.

Journal

Science 302:286-90 (2003)
DOI:10.1126/science.1086997

Reference

PMID:12022921

Authors

Sekowska A, Danchin A.

Title

The methionine salvage pathway in Bacillus subtilis.

Journal

BMC Microbiol 2:8 (2002)
DOI:10.1186/1471-2180-2-8

Reference

PMID:12670965

Authors

Berger BJ, English S, Chan G, Knodel MH.

Title

Methionine regeneration and aminotransferases in Bacillus subtilis, Bacillus cereus, and Bacillus anthracis.

Journal

J Bacteriol 185:2418-31 (2003)
DOI:10.1128/JB.185.8.2418-2431.2003

Reference

PMID:17169919

Authors

Goyer A, Collakova E, Shachar-Hill Y, Hanson AD

Title

Functional characterization of a methionine gamma-lyase in Arabidopsis and its implication in an alternative to the reverse trans-sulfuration pathway.

Journal

Plant Cell Physiol 48:232-42 (2007)
DOI:10.1093/pcp/pcl055

Reference

PMID:17030798

Authors

Rebeille F, Jabrin S, Bligny R, Loizeau K, Gambonnet B, Van Wilder V, Douce R, Ravanel S

Title

Methionine catabolism in Arabidopsis cells is initiated by a gamma-cleavage process and leads to S-methylcysteine and isoleucine syntheses.

Journal

Proc Natl Acad Sci U S A 103:15687-92 (2006)
DOI:10.1073/pnas.0606195103

Reference

PMID:18625006

Authors

Pirkov I, Norbeck J, Gustafsson L, Albers E

Title

A complete inventory of all enzymes in the eukaryotic methionine salvage pathway.

Journal

FEBS J 275:4111-20 (2008)
DOI:10.1111/j.1742-4658.2008.06552.x

Reference

PMID:18391471

Authors

Ashida H, Saito Y, Kojima C, Yokota A

Title

Enzymatic characterization of 5-methylthioribulose-1-phosphate dehydratase of the methionine salvage pathway in Bacillus subtilis.

Journal

Biosci Biotechnol Biochem 72:959-67 (2008)
DOI:10.1271/bbb.70651

Reference

PMID:10613873

Authors

Kitabatake M, So MW, Tumbula DL, Soll D

Title

Cysteine biosynthesis pathway in the archaeon Methanosarcina barkeri encoded by acquired bacterial genes?

Journal

J Bacteriol 182:143-5 (2000)
DOI:10.1128/JB.182.1.143-145.2000

Reference

PMID:12644499

Authors

Mino K, Ishikawa K

Title

Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003

Reference

PMID:18520135

Authors

Tanabe S

Title

Development of assay methods for endogenous inorganic sulfur compounds and sulfurtransferases and evaluation of the physiological functions of bound sulfur.

Journal

Yakugaku Zasshi 128:881-900 (2008)
DOI:10.1248/yakushi.128.881

Reference

Authors

Nishizuka Y, Seyama Y, Ikai A, Ishimura Y, Kawaguchi A (eds).

Title

[Cellular Functions and Metabolic Maps] (In Japanese)

Journal

Tokyo Kagaku Dojin (1997)

Reference

PMID:19270684

Authors