Entry Name Oocyte meiosis - Labeo rohita (rohu)
Description During meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, homologous chromosomes recombine and then segregate to opposite poles, while the sister chromatids segregate from each other at meoisis II. In vertebrates, immature oocytes are arrested at the PI (prophase of meiosis I). The resumption of meiosis is stimulated by progesterone, which carries the oocyte through two consecutive M-phases (MI and MII) to a second arrest at MII. The key activity driving meiotic progression is the MPF (maturation-promoting factor), a heterodimer of CDC2 (cell division cycle 2 kinase) and cyclin B. In PI-arrested oocytes, MPF is initially inactive and is activated by the dual-specificity CDC25C phosphatase as the result of new synthesis of Mos induced by progesterone. MPF activation mediates the transition from the PI arrest to MI. The subsequent decrease in MPF levels, required to exit from MI into interkinesis, is induced by a negative feedback loop, where CDC2 brings about the activation of the APC (anaphase-promoting complex), which mediates destruction of cyclin B. Re-activation of MPF for MII requires re-accumulation of high levels of cyclin B as well as the inactivation of the APC by newly synthesized Emi2 and other components of the CSF (cytostatic factor), such as cyclin E or high levels of Mos. CSF antagonizes the ubiquitin ligase activity of the APC, preventing cyclin B destruction and meiotic exit until fertilization occurs. Fertilization triggers a transient increase in cytosolic free Ca2+, which leads to CSF inactivation and cyclin B destruction through the APC. Then eggs are released from MII into the first embryonic cell cycle.
Class Cellular Processes; Cell growth and deathBRITE hierarchy
Pathway map Ortholog table Other DBs Organism Labeo rohita (rohu) [GN:
lroh ]
Gene 127159391 prkacaa; protein kinase, cAMP-dependent, catalytic, alpha, genome duplicate a isoform X1 [KO:K04345 ] [EC:2.7.11.11 ]
127176191 cpeb4a; cytoplasmic polyadenylation element-binding protein 4 isoform X1 [KO:K02602 ]
127154637 cpeb2; cytoplasmic polyadenylation element-binding protein 2 isoform X1 [KO:K02602 ]
127156465 cpeb1a; cytoplasmic polyadenylation element-binding protein 1a isoform X1 [KO:K02602 ]
127174913 cpeb3; cytoplasmic polyadenylation element-binding protein 3 isoform X1 [KO:K02602 ]
127168994 cpeb1b; cytoplasmic polyadenylation element-binding protein 1 isoform X1 [KO:K02602 ]
127152981 cpeb4b; cytoplasmic polyadenylation element-binding protein 4b isoform X1 [KO:K02602 ]
127180735 map2k1; LOW QUALITY PROTEIN: dual specificity mitogen-activated protein kinase kinase 1 [KO:K04368 ] [EC:2.7.12.2 ]
127174497 pkmyt1; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase [KO:K06633 ] [EC:2.7.11.1 ]
127178495 ywhabl; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide like [KO:K16197 ]
127179932 ywhaqb; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide b [KO:K16197 ]
127182953 ywhaqa; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide a [KO:K16197 ]
127177265 ywhae1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 1 isoform X1 [KO:K06630 ]
127168119 ywhae2; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 2 isoform X1 [KO:K06630 ]
127175976 fbxw11a; F-box and WD repeat domain-containing 11-A isoform X1 [KO:K03362 ]
127172473 fbxw11b; F-box and WD repeat domain-containing 11-B isoform X1 [KO:K03362 ]
127175267 anapc1; anaphase-promoting complex subunit 1 isoform X1 [KO:K03348 ]
127163133 cdc27; cell division cycle protein 27 homolog isoform X1 [KO:K03350 ]
127182464 anapc4; anaphase-promoting complex subunit 4 isoform X1 [KO:K03351 ]
127166549 anapc11; anaphase-promoting complex subunit 11 isoform X1 [KO:K03358 ]
127154741 smc1al; structural maintenance of chromosomes 1A, like [KO:K06636 ]
127155274 smc1a; LOW QUALITY PROTEIN: structural maintenance of chromosomes protein 1A [KO:K06636 ]
127156826 smc1b; structural maintenance of chromosomes protein 1B isoform X1 [KO:K06636 ]
127154245 smc3; structural maintenance of chromosomes protein 3 [KO:K06669 ]
127168535 mad2l1; mitotic spindle assembly checkpoint protein MAD2A [KO:K02537 ]
127169824 mad2l2; mitotic spindle assembly checkpoint protein MAD2B [KO:K13728 ]
127167518 mad1l1; mitotic spindle assembly checkpoint protein MAD1 [KO:K06679 ]
127179835 ppp2r5ca; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform isoform X1 [KO:K11584 ]
127168413 ppp2r5b; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform [KO:K11584 ]
127175010 ppp2r5d; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform [KO:K11584 ]
127175244 ppp2r5eb; protein phosphatase 2, regulatory subunit B', epsilon isoform X1 [KO:K11584 ]
127182992 ppp2r5cb; protein phosphatase 2, regulatory subunit B', gamma b isoform X1 [KO:K11584 ]
127183176 ppp2r5ea; protein phosphatase 2, regulatory subunit B', epsilon [KO:K11584 ]
127183378 ppp2r5a; serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform [KO:K11584 ]
127176846 ppp2r1bb; serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A beta isoform [KO:K03456 ]
127165263 ppp2r1ba; protein phosphatase 2, regulatory subunit A, beta a [KO:K03456 ]
127152461 ppp2cab; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform [KO:K04382 ] [EC:3.1.3.16 ]
127172757 itpr1b; LOW QUALITY PROTEIN: inositol 1,4,5-trisphosphate receptor type 1 [KO:K04958 ]
127166842 itpr1a; inositol 1,4,5-trisphosphate receptor type 1 isoform X1 [KO:K04958 ]
127169168 itpr3; inositol 1,4,5-trisphosphate receptor type 3 isoform X1 [KO:K04960 ]
127177273 calm3a; calmodulin 3a (phosphorylase kinase, delta) isoform X1 [KO:K02183 ]
127175900 ppp3cb; serine/threonine-protein phosphatase 2B catalytic subunit beta isoform isoform X1 [KO:K04348 ] [EC:3.1.3.16 ]
127165049 ppp3cca; LOW QUALITY PROTEIN: protein phosphatase 3, catalytic subunit, gamma isozyme, a [KO:K04348 ] [EC:3.1.3.16 ]
127152224 ppp3ca; serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1 [KO:K04348 ] [EC:3.1.3.16 ]
127169829 camk2d2; calcium/calmodulin-dependent protein kinase type II delta 2 chain isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
127171585 LOW QUALITY PROTEIN: calcium/calmodulin-dependent protein kinase type II subunit beta [KO:K04515 ] [EC:2.7.11.17 ]
127165937 camk2b1; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta 1 isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
127167888 camk2d1; calcium/calmodulin-dependent protein kinase type II delta 1 chain isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
127174003 camk2g1; calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma 1 isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
127174722 camk2g2; calcium/calmodulin-dependent protein kinase type II subunit gamma isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
127152422 camk2a; calcium/calmodulin-dependent protein kinase type II subunit alpha isoform X1 [KO:K04515 ] [EC:2.7.11.17 ]
Compound C01245 D-myo-Inositol 1,4,5-trisphosphate
Reference Authors Haccard O, Jessus C
Title Oocyte maturation, Mos and cyclins--a matter of synthesis: two functionally redundant ways to induce meiotic maturation.
Journal Reference Authors Crane R, Gadea B, Littlepage L, Wu H, Ruderman JV
Title Aurora A, meiosis and mitosis.
Journal Reference Authors Perdiguero E, Nebreda AR
Title Regulation of Cdc25C activity during the meiotic G2/M transition.
Journal Reference Authors Perry JA, Kornbluth S
Title Cdc25 and Wee1: analogous opposites?
Journal Reference Authors Jessus C, Ozon R
Title How does Xenopus oocyte acquire its competence to undergo meiotic maturation?
Journal Reference Authors Revenkova E, Jessberger R
Title Shaping meiotic prophase chromosomes: cohesins and synaptonemal complex proteins.
Journal Reference Authors Belloc E, Pique M, Mendez R
Title Sequential waves of polyadenylation and deadenylation define a translation circuit that drives meiotic progression.
Journal Reference Authors Pesin JA, Orr-Weaver TL
Title Regulation of APC/C activators in mitosis and meiosis.
Journal Reference Authors Tang W, Wu JQ, Guo Y, Hansen DV, Perry JA, Freel CD, Nutt L, Jackson PK, Kornbluth S
Title Cdc2 and Mos regulate Emi2 stability to promote the meiosis I-meiosis II transition.
Journal Reference Authors Wu JQ, Kornbluth S
Title Across the meiotic divide - CSF activity in the post-Emi2/XErp1 era.
Journal Reference Authors Schmidt A, Rauh NR, Nigg EA, Mayer TU
Title Cytostatic factor: an activity that puts the cell cycle on hold.
Journal Reference Authors Vogt E, Kirsch-Volders M, Parry J, Eichenlaub-Ritter U
Title Spindle formation, chromosome segregation and the spindle checkpoint in mammalian oocytes and susceptibility to meiotic error.
Journal Reference Authors Perry AC, Verlhac MH
Title Second meiotic arrest and exit in frogs and mice.
Journal Reference Authors Tunquist BJ, Eyers PA, Chen LG, Lewellyn AL, Maller JL
Title Spindle checkpoint proteins Mad1 and Mad2 are required for cytostatic factor-mediated metaphase arrest.
Journal Reference Authors Hutchins JR, Dikovskaya D, Clarke PR.
Title Regulation of Cdc2/cyclin B activation in Xenopus egg extracts via inhibitory phosphorylation of Cdc25C phosphatase by Ca(2+)/calmodulin-dependent protein [corrected] kinase II.
Journal Related lroh04914 Progesterone-mediated oocyte maturation
KO pathway LinkDB All DBs
