KEGG   PATHWAY: bta00510
Entry
bta00510                    Pathway                                
Name
N-Glycan biosynthesis - Bos taurus (cow)
Description
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].
Class
Metabolism; Glycan biosynthesis and metabolism
Pathway map
bta00510  N-Glycan biosynthesis
bta00510

Module
bta_M00072  N-glycosylation by oligosaccharyltransferase [PATH:bta00510]
bta_M00073  N-glycan precursor trimming [PATH:bta00510]
bta_M00075  N-glycan biosynthesis, complex type [PATH:bta00510]
Other DBs
GO: 0006487
Organism
Bos taurus (cow) [GN:bta]
Gene
100125314  ALG12; dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase precursor [KO:K03847] [EC:2.4.1.260]
100125390  B4GALT2; beta-1,4-galactosyltransferase 2 [KO:K07967] [EC:2.4.1.22 2.4.1.90 2.4.1.38 2.4.1.-]
100301169  MAGT1; magnesium transporter protein 1 precursor [KO:K19478]
100336900  [KO:K23741]
101906103  MGAT2; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00736] [EC:2.4.1.143]
101907024  MGAT4D; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4D isoform X1 [KO:K00738] [EC:2.4.1.145]
107131137  MAN1B1; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X2 [KO:K23741]
107132045  [KO:K07432] [EC:2.4.1.141]
281177  FUT8; alpha-(1,6)-fucosyltransferase [KO:K00717] [EC:2.4.1.68]
281781  B4GALT1; beta-1,4-galactosyltransferase 1 [KO:K07966] [EC:2.4.1.22 2.4.1.90 2.4.1.38 2.4.1.-]
282073  ST6GAL1; beta-galactoside alpha-2,6-sialyltransferase 1 [KO:K00778] [EC:2.4.3.1]
282276  MGAT4A; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A [KO:K00738] [EC:2.4.1.145]
493992  ST6GAL2; beta-galactoside alpha-2,6-sialyltransferase 2 [KO:K00779] [EC:2.4.3.1]
504346  ALG9; alpha-1,2-mannosyltransferase ALG9 isoform X1 [KO:K03846] [EC:2.4.1.259 2.4.1.261]
504474  STT3B; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]
504908  DOLPP1; dolichyldiphosphatase 1 [KO:K07252] [EC:3.6.1.43]
506537  ALG14; UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]
506566  ALG5; dolichyl-phosphate beta-glucosyltransferase isoform 2 [KO:K00729] [EC:2.4.1.117]
507210  ALG1; chitobiosyldiphosphodolichol beta-mannosyltransferase precursor [KO:K03842] [EC:2.4.1.142]
507815  STT3A; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]
507939  RPN1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor [KO:K12666]
509745  DPM3; dolichol-phosphate mannosyltransferase subunit 3 precursor [KO:K09659]
510138  ALG6; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase precursor [KO:K03848] [EC:2.4.1.267]
510682  DDOST; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor [KO:K12670]
513482  DHRSX; dehydrogenase/reductase SDR family member on chromosome X [KO:K11170] [EC:1.1.1.441]
515282  ALG13; putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 isoform X1 [KO:K07432] [EC:2.4.1.141]
515771  B4GALT3; beta-1,4-galactosyltransferase 3 [KO:K07968] [EC:2.4.1.90 2.4.1.38 2.4.1.-]
516155  MOGS; mannosyl-oligosaccharide glucosidase [KO:K01228] [EC:3.2.1.106]
520087  MGAT3; beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]
521539  MAN2A1; alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]
523737  DPM2; dolichol phosphate-mannose biosynthesis regulatory protein [KO:K09658]
526256  MGAT4B; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B precursor [KO:K00738] [EC:2.4.1.145]
526844  DOLK; dolichol kinase [KO:K00902] [EC:2.7.1.108]
527449  MAN2A2; alpha-mannosidase 2x [KO:K01231] [EC:3.2.1.114]
530027  MAN1A1; mannosyl-oligosaccharide 1,2-alpha-mannosidase IA [KO:K01230] [EC:3.2.1.113]
532645  MAN1A2; mannosyl-oligosaccharide 1,2-alpha-mannosidase IB [KO:K01230] [EC:3.2.1.113]
534097  DPM1; dolichol-phosphate mannosyltransferase subunit 1 [KO:K00721] [EC:2.4.1.83]
534231  RPN2; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 precursor [KO:K12667]
534248  MGAT1; alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]
535834  SRD5A3; polyprenol reductase [KO:K12345] [EC:1.3.1.22 1.3.1.94]
537595  MGAT5; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A [KO:K00744] [EC:2.4.1.155]
537812  DPAGT1; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]
538731  ALG8; probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase precursor [KO:K03849] [EC:2.4.1.265]
538899  ALG2; alpha-1,3/1,6-mannosyltransferase ALG2 [KO:K03843] [EC:2.4.1.132 2.4.1.257]
539661  MGAT4C; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C [KO:K13748] [EC:2.4.1.201 2.4.1.145]
539818  [KO:K12666]
539837  ALG10; dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]
540155  GANAB; neutral alpha-glucosidase AB [KO:K05546] [EC:3.2.1.207]
540389  KRTCAP2; keratinocyte-associated protein 2 [KO:K28202]
540845  ALG11; GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]
614538  DAD1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO:K12668]
614624  ALG3; dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]
615007  TUSC3; tumor suppressor candidate 3 precursor [KO:K19478]
615012  MAN1C1; mannosyl-oligosaccharide 1,2-alpha-mannosidase IC isoform X1 [KO:K01230] [EC:3.2.1.113]
767958  TMEM258; transmembrane protein 258 [KO:K26502]
768033  OSTC; oligosaccharyltransferase complex subunit OSTC [KO:K26501]
785213  MGAT5B; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B isoform X1 [KO:K09661] [EC:2.4.1.-]
Compound
C00096  GDP-mannose
C00110  Dolichyl phosphate
C00381  Dolichol
C00621  Dolichyl diphosphate
C01246  Dolichyl beta-D-glucosyl phosphate
C03021  Protein asparagine
C03862  Dolichyl phosphate D-mannose
C06081  Polyprenol
C22974  ditrans,polycis-Polyprenal
C22975  Dolichal
G00001  N-Acetyl-D-glucosaminyldiphosphodolichol
G00002  N,N'-Chitobiosyldiphosphodolichol
G00003  
G00004  
G00005  
G00006  
G00007  
G00008  
G00009  
G00010  
G00011  
G00012  
G00013  
G00014  
G00015  
G00016  Core fucose
G00017  
G00018  DS 3
G00019  
G00020  
G00021  
G00022  
G00171  
G10526  
G10595  
G10596  
G10597  
G10598  
G10599  
G10694  
Reference
PMID:9111134
  Authors
Schutzbach JS.
  Title
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
  Journal
Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002
Reference
  Authors
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
  Title
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
  Journal
Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208
Reference
  Authors
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
  Title
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
  Journal
J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200
Reference
  Authors
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
  Title
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
  Journal
J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200
Reference
  Authors
Frank CG, Aebi M.
  Title
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
  Journal
Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002
Reference
  Authors
O'Reilly MK, Zhang G, Imperiali B.
  Title
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
  Journal
Biochemistry 45:9593-603 (2006)
DOI:10.1021/bi060878o
Related
pathway
bta00051  Fructose and mannose metabolism
bta00511  Other glycan degradation
bta00513  Various types of N-glycan biosynthesis
bta00515  Mannose type O-glycan biosynthesis
bta00520  Amino sugar and nucleotide sugar metabolism
bta00533  Glycosaminoglycan biosynthesis - keratan sulfate
bta00563  Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
bta00900  Terpenoid backbone biosynthesis
KO pathway
ko00510   

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