KEGG PATHWAY: rno00510

PATHWAY: rno00510

Entry

rno00510                    Pathway

Name

N-Glycan biosynthesis - Rattus norvegicus (rat)

Description

N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:M00055], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:H00118 H00119].

Class

Metabolism; Glycan biosynthesis and metabolism

Pathway map

rno00510

N-Glycan biosynthesis

Module

rno_M00072

N-glycosylation by oligosaccharyltransferase [PATH:rno00510]

rno_M00073

N-glycan precursor trimming [PATH:rno00510]

rno_M00075

N-glycan biosynthesis, complex type [PATH:rno00510]

Other DBs

GO:

0006487

Organism

Rattus norvegicus (rat) [GN:rno]

Gene

100363048

Tmem258l5; transmembrane protein 258-like [KO:K26502]

108349244

Alg13; putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13 isoform X1 [KO:K07432] [EC:2.4.1.141]

116967

Magt1; magnesium transporter protein 1 precursor [KO:K19478]

192275

Dad1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 isoform 2 [KO:K12668]

24390

B4galt1; beta-1,4-galactosyltransferase 1 [KO:K07966] [EC:2.4.1.22 2.4.1.90 2.4.1.38 2.4.1.-]

245960

Alg10; putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO:K03850] [EC:2.4.1.256]

25197

St6gal1; beta-galactoside alpha-2,6-sialyltransferase 1 isoform 1 [KO:K00778] [EC:2.4.3.1]

25478

Man2a1; alpha-mannosidase 2 [KO:K01231] [EC:3.2.1.114]

25596

Rpn1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 precursor [KO:K12666]

287983

Alg3; dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase [KO:K03845] [EC:2.4.1.258]

288525

Dhrsx; dehydrogenase/reductase SDR family member on chromosome X isoform 2 [KO:K11170] [EC:1.1.1.441]

290783

Tusc3; tumor suppressor candidate 3 precursor [KO:K19478]

293129

Alg8; probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase precursor [KO:K03849] [EC:2.4.1.265]

293721

Ganab; neutral alpha-glucosidase AB isoform 1 [KO:K05546] [EC:3.2.1.207]

294410

Man1a1; mannosyl-oligosaccharide 1,2-alpha-mannosidase IA [KO:K01230] [EC:3.2.1.113]

295051

Alg5; dolichyl-phosphate beta-glucosyltransferase [KO:K00729] [EC:2.4.1.117]

295243

Krtcap2; keratinocyte-associated protein 2 [KO:K28202]

295319

Man1a2; mannosyl-oligosaccharide 1,2-alpha-mannosidase IB [KO:K01230] [EC:3.2.1.113]

29582

Mgat3; beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase [KO:K00737] [EC:2.4.1.144]

296394

Dpm1; dolichol-phosphate mannosyltransferase subunit 1 [KO:K00721] [EC:2.4.1.83]

29640

Dpm2; dolichol phosphate-mannose biosynthesis regulatory protein [KO:K09658]

296624

Dolpp1; dolichyldiphosphatase 1 isoform 2 [KO:K07252] [EC:3.6.1.43]

299756

Mgat4c; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase C [KO:K13748] [EC:2.4.1.201 2.4.1.145]

300284

Alg13l1; UDP-N-acetylglucosamine transferase subunit ALG13 homolog [KO:K07432] [EC:2.4.1.141]

300668

Dpagt1; UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO:K01001] [EC:2.7.8.15]

301155

St6gal2; beta-galactoside alpha-2,6-sialyltransferase 2 [KO:K00779] [EC:2.4.3.1]

303100

Mgat4b; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B precursor [KO:K00738] [EC:2.4.1.145]

303693

Mgat5b; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase B [KO:K09661] [EC:2.4.1.-]

304646

Mgat4d; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4D precursor [KO:K00738] [EC:2.4.1.145]

305291

Srd5a3; polyprenol reductase isoform 1 [KO:K12345] [EC:1.3.1.22 1.3.1.94]

308757

Man2a2; alpha-mannosidase 2x [KO:K01231] [EC:3.2.1.114]

311847

Dolk; dolichol kinase [KO:K00902] [EC:2.7.1.108]

313231

Alg2; alpha-1,3/1,6-mannosyltransferase ALG2 [KO:K03843] [EC:2.4.1.132 2.4.1.257]

313536

B4galt2; beta-1,4-galactosyltransferase 2 [KO:K07967] [EC:2.4.1.22 2.4.1.90 2.4.1.38 2.4.1.-]

313648

Ddost; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor [KO:K12670]

315212

Alg12; dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase precursor [KO:K03847] [EC:2.4.1.260]

360475

Alg1; chitobiosyldiphosphodolichol beta-mannosyltransferase precursor [KO:K03842] [EC:2.4.1.142]

361174

Alg11; GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase [KO:K03844] [EC:2.4.1.131]

362031

Alg14; UDP-N-acetylglucosamine transferase subunit ALG14 homolog [KO:K07441] [EC:2.4.1.141]

362040

Ostc; oligosaccharyltransferase complex subunit OSTC [KO:K26501]

362547

Alg6; dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase precursor [KO:K03848] [EC:2.4.1.267]

362625

Man1c1; mannosyl-oligosaccharide 1,2-alpha-mannosidase IC [KO:K01230] [EC:3.2.1.113]

363160

Stt3b; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO:K07151] [EC:2.4.99.18]

367083

Alg9; alpha-1,2-mannosyltransferase ALG9 isoform 1 [KO:K03846] [EC:2.4.1.259 2.4.1.261]

367252

Mgat4a; alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A isoform 2 [KO:K00738] [EC:2.4.1.145]

432392

Fut8; alpha-(1,6)-fucosyltransferase [KO:K00717] [EC:2.4.1.68]

494342

B4galt3; beta-1,4-galactosyltransferase 3 [KO:K07968] [EC:2.4.1.90 2.4.1.38 2.4.1.-]

499317

Tmem258; transmembrane protein 258 [KO:K26502]

499751

Man1b1; endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase [KO:K23741]

500972

Stt3a; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO:K07151] [EC:2.4.99.18]

502017

Dpm3; dolichol-phosphate mannosyltransferase subunit 3 precursor [KO:K09659]

502595

Tmem258l1; transmembrane protein 258-like [KO:K26502]

64701

Rpn2; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 precursor [KO:K12667]

65271

Mgat5; alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A [KO:K00744] [EC:2.4.1.155]

679583

Tmem258l2; transmembrane protein 258-like [KO:K26502]

680191

Tmem258l4; transmembrane protein 258-like [KO:K26502]

686092

Tmem258b; transmembrane protein 258 B [KO:K26502]

690138

Tmem258l3; transmembrane protein 258-like [KO:K26502]

78947

Mogs; mannosyl-oligosaccharide glucosidase [KO:K01228] [EC:3.2.1.106]

81519

Mgat1; alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase [KO:K00726] [EC:2.4.1.101]

94273

Mgat2; alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase precursor [KO:K00736] [EC:2.4.1.143]

Compound

C00096

GDP-mannose

C00110

Dolichyl phosphate

C00381

Dolichol

C00621

Dolichyl diphosphate

C01246

Dolichyl beta-D-glucosyl phosphate

C03021

Protein asparagine

C03862

Dolichyl phosphate D-mannose

C06081

Polyprenol

C22974

ditrans,polycis-Polyprenal

C22975

Dolichal

G00001

N-Acetyl-D-glucosaminyldiphosphodolichol

G00002

N,N'-Chitobiosyldiphosphodolichol

G00003

G00004

G00005

G00006

G00007

G00008

G00009

G00010

G00011

G00012

G00013

G00014

G00015

G00016

Core fucose

G00017

G00018

DS 3

G00019

G00020

G00021

G00022

G00171

G10526

G10595

G10596

G10597

G10598

G10599

G10694

Reference

PMID:9111134

Authors

Schutzbach JS.

Title

The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.

Journal

Glycoconj J 14:175-82 (1997)
DOI:10.1023/A:1018533620002

Reference

PMID:12200473

Authors

Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.

Title

Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.

Journal

Mol Biol Evol 19:1451-63 (2002)
DOI:10.1093/oxfordjournals.molbev.a004208

Reference

PMID:12684507

Authors

Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.

Title

A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.

Journal

J Biol Chem 278:22498-505 (2003)
DOI:10.1074/jbc.M302850200

Reference

PMID:11278778

Authors

Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.

Title

The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.

Journal

J Biol Chem 276:21828-40 (2001)
DOI:10.1074/jbc.M010896200

Reference

PMID:15987956

Authors

Frank CG, Aebi M.

Title

ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.

Journal

Glycobiology 15:1156-63 (2005)
DOI:10.1093/glycob/cwj002

Reference

PMID:16878994