Entry Name Spliceosome - Bos taurus (cow)
Description After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.
Class Genetic Information Processing; TranscriptionBRITE hierarchy
Pathway map Other DBs Organism Bos taurus (cow) [GN:
bta ]
Gene 100124428 NCBP1; nuclear cap-binding protein subunit 1 isoform X1 [KO:K12882 ]
100301114 PRPF40A; pre-mRNA-processing factor 40 homolog A isoform X6 [KO:K12821 ]
107132038 NCBP2L; LOW QUALITY PROTEIN: nuclear cap-binding protein subunit 2-like [KO:K12883 ]
505019 U2SURP; U2 snRNP-associated SURP motif-containing protein isoform X1 [KO:K12842 ]
505093 HNRNPA1; heterogeneous nuclear ribonucleoprotein A1 [KO:K12741 ]
506405 DHX16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 [KO:K12813 ] [EC:5.6.2.6 ]
507066 SRSF7; serine/arginine-rich splicing factor 7 [KO:K12896 ]
507180 PHF5A; PHD finger-like domain-containing protein 5A [KO:K12834 ]
507371 PRPF8; pre-mRNA-processing-splicing factor 8 [KO:K12856 ]
507828 SRSF6; serine/arginine-rich splicing factor 6 [KO:K12893 ]
507895 U2AF2; splicing factor U2AF 65 kDa subunit [KO:K12837 ]
508312 SRSF2; serine/arginine-rich splicing factor 2 [KO:K12891 ]
508735 PRPF4; U4/U6 small nuclear ribonucleoprotein Prp4 [KO:K12662 ]
508788 SNRPD1; small nuclear ribonucleoprotein Sm D1 [KO:K11087 ]
509425 EFTUD2; 116 kDa U5 small nuclear ribonucleoprotein component [KO:K12852 ]
509591 RBMX; RNA-binding motif protein, X chromosome [KO:K12885 ]
509802 SNRPA; U1 small nuclear ribonucleoprotein A [KO:K11091 ]
509905 SRSF9; serine/arginine-rich splicing factor 9 [KO:K21123 ]
509920 LSM7; U6 snRNA-associated Sm-like protein LSm7 [KO:K12626 ]
510322 PRPF3; U4/U6 small nuclear ribonucleoprotein Prp3 [KO:K12843 ]
510474 SRSF5; serine/arginine-rich splicing factor 5 [KO:K12893 ]
510852 HNRNPC; heterogeneous nuclear ribonucleoproteins C1/C2 [KO:K12884 ]
510872 NCBP2; nuclear cap-binding protein subunit 2 [KO:K12883 ]
512527 CWC25; pre-mRNA-splicing factor CWC25 homolog [KO:K28450 ]
512584 SNRPA1; U2 small nuclear ribonucleoprotein A' [KO:K11092 ]
512680 U2AF1; splicing factor U2AF 35 kDa subunit [KO:K12836 ]
512824 PUF60; poly(U)-binding-splicing factor PUF60 isoform 3 [KO:K12838 ]
513577 PRPF31; U4/U6 small nuclear ribonucleoprotein Prp31 [KO:K12844 ]
513782 PRPF40B; pre-mRNA-processing factor 40 homolog B isoform X7 [KO:K12821 ]
514932 SNRPD2; small nuclear ribonucleoprotein Sm D2 [KO:K11096 ]
515290 HNRNPM; heterogeneous nuclear ribonucleoprotein M [KO:K12887 ]
520046 DHX38; pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 [KO:K12815 ] [EC:5.6.2.6 ]
520500 SMNDC1; survival of motor neuron-related-splicing factor 30 [KO:K12839 ]
525110 CHERP; calcium homeostasis endoplasmic reticulum protein [KO:K12841 ]
525944 SNIP1; smad nuclear-interacting protein 1 [KO:K13108 ]
528135 HNRNPK; heterogeneous nuclear ribonucleoprotein K [KO:K12886 ]
532985 USP39; U4/U6.U5 tri-snRNP-associated protein 2 [KO:K12847 ]
534586 TRA2A; transformer-2 protein homolog alpha [KO:K12897 ]
534600 ACIN1; apoptotic chromatin condensation inducer in the nucleus isoform X1 [KO:K12875 ]
534645 SNRNP40; U5 small nuclear ribonucleoprotein 40 kDa protein [KO:K12857 ]
535113 SNRNP70; U1 small nuclear ribonucleoprotein 70 kDa [KO:K11093 ]
535258 CWC15; spliceosome-associated protein CWC15 homolog [KO:K12863 ]
536745 GPKOW; G-patch domain and KOW motifs-containing protein [KO:K13101 ]
538662 LSM2; U6 snRNA-associated Sm-like protein LSm2 [KO:K12621 ]
539950 HNRNPU; heterogeneous nuclear ribonucleoprotein U [KO:K12888 ]
540276 SRSF3; serine/arginine-rich splicing factor 3 [KO:K12892 ]
540964 TCERG1; transcription elongation regulator 1 isoform X8 [KO:K12824 ]
613304 SNRPB; small nuclear ribonucleoprotein-associated protein B' [KO:K11086 ]
613630 LSM8; LSM8 homolog, U6 small nuclear RNA associated [KO:K12627 ]
613634 LSM4; U6 snRNA-associated Sm-like protein LSm4 [KO:K12623 ]
613705 RBMX2; RNA-binding motif protein, X-linked 2 [KO:K13107 ]
614310 SRSF4; serine/arginine-rich splicing factor 4 [KO:K12893 ]
614553 SNRPC; U1 small nuclear ribonucleoprotein C [KO:K11095 ]
614759 LSM3; U6 snRNA-associated Sm-like protein LSm3 [KO:K12622 ]
615136 LSM5; U6 snRNA-associated Sm-like protein LSm5 [KO:K12624 ]
615156 TRA2B; transformer-2 protein homolog beta [KO:K12897 ]
615198 U2AF1L4; splicing factor U2AF 26 kDa subunit [KO:K12836 ]
615312 RP9; retinitis pigmentosa 9 protein isoform X1 [KO:K19604 ]
615704 SART1; U4/U6.U5 tri-snRNP-associated protein 1 [KO:K11984 ]
615796 SRSF1; serine/arginine-rich splicing factor 1 [KO:K12890 ]
615916 LSM6; U6 snRNA-associated Sm-like protein LSm6 [KO:K12625 ]
617519 CCDC12; coiled-coil domain-containing protein 12 [KO:K12871 ]
617823 SNRPD3; small nuclear ribonucleoprotein Sm D3 [KO:K11088 ]
767817 CDC5L; cell division cycle 5-like protein [KO:K12860 ]
767873 SNRPB2; U2 small nuclear ribonucleoprotein B'' [KO:K11094 ]
768310 SRSF10; serine/arginine-rich splicing factor 10 [KO:K12900 ]
782669 HNRNPA3; heterogeneous nuclear ribonucleoprotein A3 [KO:K12741 ]
783414 ISY1; pre-mRNA-splicing factor ISY1 homolog [KO:K12870 ]
790677 SNRNP27; U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [KO:K12846 ]
Reference Authors Wahl MC, Will CL, Luhrmann R
Title The spliceosome: design principles of a dynamic RNP machine.
Journal Reference Authors Morris DP, Greenleaf AL
Title The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Journal Reference Authors Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Title Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Journal Reference Authors Ritchie DB, Schellenberg MJ, MacMillan AM
Title Spliceosome structure: piece by piece.
Journal Reference Authors Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Title Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Journal Reference Authors Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Title Structure of the human activated spliceosome in three conformational states.
Journal Reference Authors Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Title Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.
Journal Reference Authors Zhan X, Lu Y, Shi Y
Title Molecular basis for the activation of human spliceosome.
Journal KO pathway
