Entry
Name
Spliceosome - Peromyscus leucopus (white-footed mouse)
Description
After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.
Class
Genetic Information Processing; Transcription
BRITE hierarchy
Pathway map
Other DBs
Organism
Peromyscus leucopus (white-footed mouse) [GN:
pleu ]
Gene
114680752 Snrnp27; U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [KO:K12846 ]
114681619 Srsf7; serine/arginine-rich splicing factor 7 isoform X3 [KO:K12896 ]
114681659 U2af1l4; splicing factor U2AF 26 kDa subunit isoform X1 [KO:K12836 ]
114682233 Hnrnpc; heterogeneous nuclear ribonucleoproteins C1/C2 isoform X1 [KO:K12884 ]
114682386 Prpf3; U4/U6 small nuclear ribonucleoprotein Prp3 isoform X1 [KO:K12843 ]
114682399 Sf3b4; LOW QUALITY PROTEIN: splicing factor 3B subunit 4 [KO:K12831 ]
114684732 Puf60; poly(U)-binding-splicing factor PUF60 isoform X1 [KO:K12838 ]
114684796 U2af2; splicing factor U2AF 65 kDa subunit isoform X1 [KO:K12837 ]
114685322 Hnrnpm; heterogeneous nuclear ribonucleoprotein M isoform X2 [KO:K12887 ]
114687003 Dhx16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 isoform X1 [KO:K12813 ] [EC:5.6.2.6 ]
114688602 Hnrnpk; heterogeneous nuclear ribonucleoprotein K isoform X1 [KO:K12886 ]
114690029 Hspa6; LOW QUALITY PROTEIN: heat shock 70 kDa protein 6 [KO:K03283 ]
114691434 Prpf8; LOW QUALITY PROTEIN: pre-mRNA-processing-splicing factor 8 [KO:K12856 ]
114694713 Lsm8; LSM8 homolog, U6 small nuclear RNA associated isoform X1 [KO:K12627 ]
114695482 Snrpa1; LOW QUALITY PROTEIN: U2 small nuclear ribonucleoprotein A' [KO:K11092 ]
114696605 Hnrnpa1; heterogeneous nuclear ribonucleoprotein A1 isoform X1 [KO:K12741 ]
114696694 Prpf40b; pre-mRNA-processing factor 40 homolog B isoform X1 [KO:K12821 ]
114697044 Smndc1; survival of motor neuron-related-splicing factor 30 isoform X1 [KO:K12839 ]
114697235 Srsf10; serine/arginine-rich splicing factor 10 isoform X1 [KO:K12900 ]
114697614 Prpf40a; pre-mRNA-processing factor 40 homolog A isoform X1 [KO:K12821 ]
114697792 Srsf5; serine/arginine-rich splicing factor 5 isoform X1 [KO:K12893 ]
114698169 Snrpd1; small nuclear ribonucleoprotein Sm D1 isoform X1 [KO:K11087 ]
114699485 Snrpb; small nuclear ribonucleoprotein-associated proteins B and B' [KO:K11086 ]
114700819 Hnrnpa3; heterogeneous nuclear ribonucleoprotein A3 isoform X1 [KO:K12741 ]
114701288 Gpkow; G-patch domain and KOW motifs-containing protein [KO:K13101 ]
114701599 Tcerg1; transcription elongation regulator 1 isoform X2 [KO:K12824 ]
114702243 Eftud2; 116 kDa U5 small nuclear ribonucleoprotein component isoform X1 [KO:K12852 ]
114702359 Ncbp1; nuclear cap-binding protein subunit 1 isoform X1 [KO:K12882 ]
114704002 U2af1; splicing factor U2AF 35 kDa subunit isoform X1 [KO:K12836 ]
114705655 Snrnp40; U5 small nuclear ribonucleoprotein 40 kDa protein isoform X1 [KO:K12857 ]
114705891 Lsm2; U6 snRNA-associated Sm-like protein LSm2 isoform X1 [KO:K12621 ]
114708752 Acin1; apoptotic chromatin condensation inducer in the nucleus isoform X1 [KO:K12875 ]
114709148 Sart1; LOW QUALITY PROTEIN: U4/U6.U5 tri-snRNP-associated protein 1 [KO:K11984 ]
Reference
Authors
Wahl MC, Will CL, Luhrmann R
Title
The spliceosome: design principles of a dynamic RNP machine.
Journal
Reference
Authors
Morris DP, Greenleaf AL
Title
The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Journal
Reference
Authors
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Title
Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Journal
Reference
Authors
Ritchie DB, Schellenberg MJ, MacMillan AM
Title
Spliceosome structure: piece by piece.
Journal
Reference
Authors
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Journal
Reference
Authors
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Title
Structure of the human activated spliceosome in three conformational states.
Journal
Reference
Authors
Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.
Journal
Reference
Authors
Zhan X, Lu Y, Shi Y
Title
Molecular basis for the activation of human spliceosome.
Journal
KO pathway