N-Glycan biosynthesis - Oryza sativa japonica (Japanese rice) (RefSeq)
N-glycans or asparagine-linked glycans are major constituents of glycoproteins in eukaryotes. N-glycans are covalently attached to asparagine with the consensus sequence of Asn-X-Ser/Thr by an N-glycosidic bond, GlcNAc b1- Asn. Biosynthesis of N-glycans begins on the cytoplasmic face of the ER membrane with the transferase reaction of UDP-GlcNAc and the lipid-like precursor P-Dol (dolichol phosphate) to generate GlcNAc a1- PP-Dol. After sequential addition of monosaccharides by ALG glycosyltransferases [MD:
], the N-glycan precursor is attached by the OST (oligosaccharyltransferase) complex to the polypeptide chain that is being synthesized and translocated through the ER membrane. The protein-bound N-glycan precursor is subsequently trimmed, extended, and modified in the ER and Golgi by a complex series of reactions catalyzed by membrane-bound glycosidases and glycosyltransferases. N-glycans thus synthesized are classified into three types: high-mannose type, complex type, and hybrid type. Defects in N-glycan biosynthesis lead to a variety of human diseases known as congenital disorders of glycosylation [DS:
Metabolism; Glycan biosynthesis and metabolism
Pathway map Ortholog table
Oryza sativa japonica (Japanese rice) (RefSeq) [GN:
4344180 UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase [KO: K01001] [EC: 18.104.22.168]
9269261 dolichol phosphate-mannose biosynthesis regulatory protein [KO: K09658]
107276486 dolichol phosphate-mannose biosynthesis regulatory protein-like [KO: K09658]
9272161 dolichol-phosphate mannosyltransferase subunit 3 [KO: K09659]
4330363 probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase [KO: K03849] [EC: 22.214.171.1245]
4336325 putative Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase [KO: K03850] [EC: 126.96.36.1996]
4337389 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B [KO: K07151] [EC: 188.8.131.52]
4339345 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A [KO: K07151] [EC: 184.108.40.206]
4338348 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1A [KO: K12666]
107275757 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1B [KO: K12666]
4324876 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 [KO: K12667]
4335701 dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 [KO: K12668]
4333265 probable dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 [KO: K12669]
4342702 dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit [KO: K12670]
4331263 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase isoform X1 [KO: K00726] [EC: 220.127.116.11]
4329853 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase isoform X1 [KO: K00737] [EC: 18.104.22.168]
C01246 Dolichyl beta-D-glucosyl phosphate
C03862 Dolichyl phosphate D-mannose
The role of the lipid matrix in the biosynthesis of dolichyl-linked oligosaccharides.
Oriol R, Martinez-Duncker I, Chantret I, Mollicone R, Codogno P.
Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate.
Thiel C, Schwarz M, Peng J, Grzmil M, Hasilik M, Braulke T, Kohlschutter A, von Figura K, Lehle L, Korner C.
A new type of congenital disorders of glycosylation (CDG-Ii) provides new insights into the early steps of dolichol-linked oligosaccharide biosynthesis.
Cipollo JF, Trimble RB, Chi JH, Yan Q, Dean N.
The yeast ALG11 gene specifies addition of the terminal alpha 1,2-Man to the Man5GlcNAc2-PP-dolichol N-glycosylation intermediate formed on the cytosolic side of the endoplasmic reticulum.
Frank CG, Aebi M.
ALG9 mannosyltransferase is involved in two different steps of lipid-linked oligosaccharide biosynthesis.
O'Reilly MK, Zhang G, Imperiali B.
In vitro evidence for the dual function of Alg2 and Alg11: essential mannosyltransferases in N-linked glycoprotein biosynthesis.
osa00051 Fructose and mannose metabolism
osa00513 Various types of N-glycan biosynthesis
osa00520 Amino sugar and nucleotide sugar metabolism
osa00563 Glycosylphosphatidylinositol (GPI)-anchor biosynthesis
osa00900 Terpenoid backbone biosynthesis
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