KEGG   PATHWAY: mun04510
Entry
mun04510                    Pathway                                
Name
Focal adhesion - Meriones unguiculatus (Mongolian gerbil)
Description
Cell-matrix adhesions play essential roles in important biological processes including cell motility, cell proliferation, cell differentiation, regulation of gene expression and cell survival. At the cell-extracellular matrix contact points, specialized structures are formed and termed focal adhesions, where bundles of actin filaments are anchored to transmembrane receptors of the integrin family through a multi-molecular complex of junctional plaque proteins. Some of the constituents of focal adhesions participate in the structural link between membrane receptors and the actin cytoskeleton, while others are signalling molecules, including different protein kinases and phosphatases, their substrates, and various adapter proteins. Integrin signaling is dependent upon the non-receptor tyrosine kinase activities of the FAK and src proteins as well as the adaptor protein functions of FAK, src and Shc to initiate downstream signaling events. These signalling events culminate in reorganization of the actin cytoskeleton; a prerequisite for changes in cell shape and motility, and gene expression. Similar morphological alterations and modulation of gene expression are initiated by the binding of growth factors to their respective receptors, emphasizing the considerable crosstalk between adhesion- and growth factor-mediated signalling.
Class
Cellular Processes; Cellular community - eukaryotes
Pathway map
mun04510  Focal adhesion
mun04510

Other DBs
GO: 0005925
Organism
Meriones unguiculatus (Mongolian gerbil) [GN:mun]
Gene
110539385  Vegfd; vascular endothelial growth factor D isoform X3 [KO:K05449]
110539539  Egfr; epidermal growth factor receptor [KO:K04361] [EC:2.7.10.1]
110539680  [KO:K12757]
110539681  [KO:K12757]
110540164  Col4a5; LOW QUALITY PROTEIN: collagen alpha-5(IV) chain [KO:K06237]
110540260  Pdgfd; platelet-derived growth factor D isoform X1 [KO:K05450]
110540522  Pak5; serine/threonine-protein kinase PAK 5 [KO:K05736] [EC:2.7.11.1]
110540728  [KO:K04513]
110541029  Parva; alpha-parvin [KO:K06275]
110541384  Pak3; serine/threonine-protein kinase PAK 3 isoform X1 [KO:K05733] [EC:2.7.11.1]
110541403  Itga10; integrin alpha-10 [KO:K06586]
110541556  Rock2; rho-associated protein kinase 2 [KO:K17388] [EC:2.7.11.39]
110542115  Xiap; E3 ubiquitin-protein ligase XIAP [KO:K04725] [EC:2.3.2.27]
110542126  Vegfc; vascular endothelial growth factor C [KO:K05449]
110542391  Ptk2; focal adhesion kinase 1 isoform X4 [KO:K05725] [EC:2.7.10.2]
110542507  Pdgfc; platelet-derived growth factor C isoform X1 [KO:K05450]
110543001  Elk1; ETS domain-containing protein Elk-1 [KO:K04375]
110545010  Cdc42; cell division control protein 42 homolog isoform X2 [KO:K04393]
110545020  Parvg; gamma-parvin isoform X1 [KO:K06275]
110545029  Parvb; beta-parvin isoform X1 [KO:K06275]
110545313  Ppp1cc; serine/threonine-protein phosphatase PP1-gamma catalytic subunit [KO:K06269] [EC:3.1.3.16]
110545344  Myl2; myosin regulatory light chain 2, ventricular/cardiac muscle isoform [KO:K10351]
110545583  Pxn; paxillin isoform X1 [KO:K05760]
110545611  Vav1; proto-oncogene vav isoform X1 [KO:K05730]
110545659  Itgb3; integrin beta-3 [KO:K06493]
110545779  Braf; serine/threonine-protein kinase B-raf isoform X8 [KO:K04365] [EC:2.7.11.1]
110546082  Myl7; myosin regulatory light chain 2, atrial isoform isoform X2 [KO:K12754]
110546189  Lamc1; laminin subunit gamma-1 [KO:K05635]
110546194  Lamc2; laminin subunit gamma-2 [KO:K06246]
110546347  Col6a2; collagen alpha-2(VI) chain isoform X1 [KO:K06238]
110546356  Col6a1; LOW QUALITY PROTEIN: collagen alpha-1(VI) chain [KO:K06238]
110546596  Actn1; alpha-actinin-1 isoform X1 [KO:K05699]
110546658  Capn2; calpain-2 catalytic subunit [KO:K03853] [EC:3.4.22.53]
110546695  Pdgfrb; platelet-derived growth factor receptor beta [KO:K05089] [EC:2.7.10.1]
110546900  Emp1; epithelial membrane protein 1 [KO:K26255]
110546940  Ccnd1; G1/S-specific cyclin-D1 isoform X2 [KO:K04503]
110546968  Pgf; placenta growth factor [KO:K16859]
110547073  Sos1; son of sevenless homolog 1 isoform X1 [KO:K03099]
110547228  Mylk; myosin light chain kinase, smooth muscle isoform X1 [KO:K00907] [EC:2.7.11.18]
110547261  Igf1r; insulin-like growth factor 1 receptor isoform X1 [KO:K05087] [EC:2.7.10.1]
110547315  Pak1; serine/threonine-protein kinase PAK 1 [KO:K04409] [EC:2.7.11.1]
110547367  Crk; adapter molecule crk isoform X1 [KO:K04438]
110547372  Flt4; vascular endothelial growth factor receptor 3 isoform X1 [KO:K05097] [EC:2.7.10.1]
110547507  Col4a4; collagen alpha-4(IV) chain [KO:K06237]
110547508  Col4a3; collagen alpha-3(IV) chain [KO:K06237]
110547752  Vav3; guanine nucleotide exchange factor VAV3 isoform X1 [KO:K05730]
110547825  Grb2; growth factor receptor-bound protein 2 [KO:K04364]
110547925  Itgb4; integrin beta-4 isoform X3 [KO:K06525]
110547978  Mapk3; mitogen-activated protein kinase 3 [KO:K04371] [EC:2.7.11.24]
110548007  Myl11; myosin regulatory light chain 11 [KO:K12758]
110548321  Cav3; caveolin-3 [KO:K12959]
110548394  Pik3cb; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform isoform X1 [KO:K00922] [EC:2.7.1.153]
110548466  Rac2; ras-related C3 botulinum toxin substrate 2 [KO:K07860]
110548510  Pip5k1c; phosphatidylinositol 4-phosphate 5-kinase type-1 gamma isoform X1 [KO:K00889] [EC:2.7.1.68]
110548579  Vwf; von Willebrand factor [KO:K03900]
110548628  Ccnd2; G1/S-specific cyclin-D2 [KO:K10151]
110548804  Actg1; actin, cytoplasmic 2 [KO:K05692]
110548820  Rac3; ras-related C3 botulinum toxin substrate 3 [KO:K07861]
110548883  Col4a2; collagen alpha-2(IV) chain [KO:K06237]
110548884  Col4a1; collagen alpha-1(IV) chain [KO:K06237]
110549145  Pik3r2; phosphatidylinositol 3-kinase regulatory subunit beta [KO:K02649]
110549169  Comp; cartilage oligomeric matrix protein [KO:K04659]
110549282  Tln2; talin-2 isoform X1 [KO:K06271]
110549494  Rasgrf1; ras-specific guanine nucleotide-releasing factor 1 isoform X1 [KO:K04349]
110549551  Rap1a; ras-related protein Rap-1A [KO:K04353]
110549884  Itgb6; integrin beta-6 [KO:K06589]
110549942  Col9a2; collagen alpha-2(IX) chain isoform X1 [KO:K08131]
110550095  Mylk2; myosin light chain kinase 2, skeletal/cardiac muscle [KO:K00907] [EC:2.7.11.18]
110550131  Vtn; vitronectin isoform X2 [KO:K06251]
110550148  [KO:K12757]
110550171  Col6a5; collagen alpha-5(VI) chain [KO:K06238]
110550186  Col6a6; collagen alpha-6(VI) chain [KO:K06238]
110550189  [KO:K06238]
110550225  Ccnd3; G1/S-specific cyclin-D3 [KO:K10152]
110550366  Vegfa; vascular endothelial growth factor A, long form isoform X3 [KO:K05448]
110550406  Itgb5; integrin beta-5 isoform X1 [KO:K06588]
110550539  Itga11; integrin alpha-11 [KO:K06587]
110550597  Pak6; serine/threonine-protein kinase PAK 6 [KO:K05735] [EC:2.7.11.1]
110550699  Pdgfa; platelet-derived growth factor subunit A isoform X3 [KO:K04359]
110550814  Raf1; RAF proto-oncogene serine/threonine-protein kinase isoform X2 [KO:K04366] [EC:2.7.11.1]
110550937  Shc4; SHC-transforming protein 4 [KO:K17449]
110551197  Itga3; integrin alpha-3 isoform X2 [KO:K06482]
110551201  Col1a1; collagen alpha-1(I) chain [KO:K06236]
110551355  Shc2; SHC-transforming protein 2 isoform X4 [KO:K17447]
110551519  Vasp; vasodilator-stimulated phosphoprotein isoform X1 [KO:K06274]
110551574  Shc1; SHC-transforming protein 1 isoform X1 [KO:K06279]
110551598  Vegfb; vascular endothelial growth factor B isoform X1 [KO:K16858]
110551603  Bad; bcl2-associated agonist of cell death isoform X1 [KO:K02158]
110551893  Itga2b; integrin alpha-IIb isoform X1 [KO:K06476]
110551998  Vcl; vinculin isoform X1 [KO:K05700]
110552062  Chad; chondroadherin [KO:K06248]
110552091  Rapgef1; rap guanine nucleotide exchange factor 1 isoform X22 [KO:K06277]
110552201  Itga4; integrin alpha-4 [KO:K06483]
110552285  Mapk8; mitogen-activated protein kinase 8 isoform X1 [KO:K04440] [EC:2.7.11.24]
110552295  Lama1; laminin subunit alpha-1 [KO:K05637]
110552301  Itgb7; integrin beta-7 [KO:K06590]
110552530  Actn4; alpha-actinin-4 isoform X1 [KO:K05699]
110552542  Pak4; serine/threonine-protein kinase PAK 4 [KO:K05734] [EC:2.7.11.1]
110552573  Thbs2; thrombospondin-2 [KO:K04659]
110552608  Cav1; caveolin-1 isoform X1 [KO:K06278]
110552609  Cav2; caveolin-2 [KO:K12958]
110552822  Dock1; dedicator of cytokinesis protein 1 isoform X1 [KO:K13708]
110552995  Mylk3; myosin light chain kinase 3 isoform X1 [KO:K00907] [EC:2.7.11.18]
110553013  Pdpk1; 3-phosphoinositide-dependent protein kinase 1 [KO:K06276] [EC:2.7.11.1]
110553196  Egf; pro-epidermal growth factor [KO:K04357]
110553293  Pak2; serine/threonine-protein kinase PAK 2 [KO:K04410] [EC:2.7.11.1]
110553478  Lama3; laminin subunit alpha-3 isoform X1 [KO:K06240]
110553533  Col9a3; collagen alpha-3(IX) chain [KO:K08131]
110553683  Pik3r1; phosphatidylinositol 3-kinase regulatory subunit alpha isoform X1 [KO:K02649]
110553751  Birc3; baculoviral IAP repeat-containing protein 3 isoform X1 [KO:K16060]
110553757  Birc2; baculoviral IAP repeat-containing protein 2 isoform X4 [KO:K16060]
110553796  Mapk1; mitogen-activated protein kinase 1 isoform X2 [KO:K04371] [EC:2.7.11.24]
110553931  Hras; GTPase HRas isoform X1 [KO:K02833]
110554009  Lama5; laminin subunit alpha-5 [KO:K06240]
110554354  Tnr; tenascin-R isoform X2 [KO:K06252]
110554524  Ppp1cb; serine/threonine-protein phosphatase PP1-beta catalytic subunit [KO:K06269] [EC:3.1.3.16]
110554614  Flnb; filamin-B isoform X1 [KO:K27392]
110554744  Bcar1; breast cancer anti-estrogen resistance protein 1 isoform X4 [KO:K05726]
110554808  Reln; reelin isoform X1 [KO:K06249] [EC:3.4.21.-]
110554841  Itgb1; integrin beta-1 isoform X2 [KO:K05719]
110555275  Emp2; epithelial membrane protein 2 [KO:K26256]
110555335  Prkca; protein kinase C alpha type [KO:K02677] [EC:2.7.11.13]
110555390  Gsk3b; glycogen synthase kinase-3 beta isoform X4 [KO:K03083] [EC:2.7.11.26]
110555733  Col9a1; collagen alpha-1(IX) chain [KO:K08131]
110556016  Prkcg; protein kinase C gamma type [KO:K19663] [EC:2.7.11.13]
110556252  Pik3ca; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform [KO:K00922] [EC:2.7.1.153]
110556719  Akt2; RAC-beta serine/threonine-protein kinase isoform X1 [KO:K04456] [EC:2.7.11.1]
110556868  Col1a2; collagen alpha-2(I) chain [KO:K06236]
110556884  Thbs3; thrombospondin-3 isoform X2 [KO:K04659]
110556913  Sos2; son of sevenless homolog 2 isoform X1 [KO:K03099]
110556939  Myl9; myosin regulatory light polypeptide 9 [KO:K12755]
110556944  Kdr; vascular endothelial growth factor receptor 2 [KO:K05098] [EC:2.7.10.1]
110557092  Diaph1; protein diaphanous homolog 1 isoform X1 [KO:K05740]
110557238  Myl10; myosin regulatory light chain 10 isoform X2 [KO:K12756]
110557288  Fn1; fibronectin isoform X1 [KO:K05717]
110557386  Mapk9; mitogen-activated protein kinase 9 isoform X1 [KO:K04440] [EC:2.7.11.24]
110557417  Rac1; ras-related C3 botulinum toxin substrate 1 isoform X1 [KO:K04392]
110557506  Vav2; guanine nucleotide exchange factor VAV2 isoform X1 [KO:K05730]
110557599  Itga5; integrin alpha-5 isoform X1 [KO:K06484]
110557688  Itga9; integrin alpha-9 [KO:K06585]
110557800  Bcl2; apoptosis regulator Bcl-2 [KO:K02161]
110557819  Pdgfb; platelet-derived growth factor subunit B isoform X1 [KO:K17386]
110558105  Pdgfra; platelet-derived growth factor receptor alpha [KO:K04363] [EC:2.7.10.1]
110558188  Actb; actin, cytoplasmic 1 [KO:K05692]
110558205  Ppp1r12a; protein phosphatase 1 regulatory subunit 12A isoform X1 [KO:K06270]
110558416  Zyx; zyxin isoform X1 [KO:K06273]
110558635  Mylk4; myosin light chain kinase family member 4 [KO:K00907] [EC:2.7.11.18]
110558703  Met; hepatocyte growth factor receptor [KO:K05099] [EC:2.7.10.1]
110558966  Akt1; RAC-alpha serine/threonine-protein kinase [KO:K04456] [EC:2.7.11.1]
110558972  Itgb8; integrin beta-8 isoform X1 [KO:K06591]
110558987  Lamb1; laminin subunit beta-1 [KO:K05636]
110559000  Map2k1; dual specificity mitogen-activated protein kinase kinase 1 [KO:K04368] [EC:2.7.12.2]
110559069  Shc3; SHC-transforming protein 3 [KO:K17448]
110559159  Itgav; integrin alpha-V [KO:K06487]
110559213  Crkl; crk-like protein [KO:K04438]
110559223  Lama2; laminin subunit alpha-2 [KO:K05637]
110559252  Lamc3; laminin subunit gamma-3 isoform X1 [KO:K06247]
110559582  Ilk; integrin-linked protein kinase isoform X1 [KO:K06272] [EC:2.7.11.1]
110559612  Prkcb; protein kinase C beta type isoform X1 [KO:K19662] [EC:2.7.11.13]
110559727  Jun; transcription factor Jun [KO:K04448]
110559812  Lamb3; laminin subunit beta-3 isoform X3 [KO:K06244]
110560093  Col2a1; collagen alpha-1(II) chain isoform X1 [KO:K19719]
110560104  Tnxb; tenascin-X isoform X1 [KO:K06252]
110560352  Ppp1ca; serine/threonine-protein phosphatase PP1-alpha catalytic subunit [KO:K06269] [EC:3.1.3.16]
110560780  Pip5k1a; phosphatidylinositol 4-phosphate 5-kinase type-1 alpha isoform X3 [KO:K00889] [EC:2.7.1.68]
110561016  Fyn; tyrosine-protein kinase Fyn isoform X3 [KO:K05703] [EC:2.7.10.2]
110561072  Ppp1r12c; protein phosphatase 1 regulatory subunit 12C isoform X1 [KO:K17457]
110561085  Itga2; integrin alpha-2 [KO:K06481]
110561134  Col6a3; collagen alpha-3(VI) chain isoform X1 [KO:K06238]
110561175  Pten; phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN [KO:K01110] [EC:3.1.3.16 3.1.3.48 3.1.3.67]
110561260  Pip5k1b; phosphatidylinositol 4-phosphate 5-kinase type-1 beta [KO:K00889] [EC:2.7.1.68]
110561302  Thbs4; thrombospondin-4 [KO:K04659]
110561313  Arhgap35; rho GTPase-activating protein 35 [KO:K05732]
110561393  Itga8; integrin alpha-8 isoform X1 [KO:K06584]
110561622  Igf1; insulin-like growth factor I isoform X5 [KO:K05459]
110561755  Ctnnb1; catenin beta-1 [KO:K02105]
110561879  Tln1; talin-1 isoform X2 [KO:K06271]
110562151  Rap1b; ras-related protein Rap-1b [KO:K07836]
110562530  Flna; filamin-A isoform X1 [KO:K04437]
110562821  Spp1; osteopontin [KO:K06250]
110562822  Ibsp; bone sialoprotein 2 [KO:K06253]
110562875  Mapk10; mitogen-activated protein kinase 10 isoform X1 [KO:K04440] [EC:2.7.11.24]
110562952  Erbb2; receptor tyrosine-protein kinase erbB-2 [KO:K05083] [EC:2.7.10.1]
110563160  Itga7; integrin alpha-7 isoform X1 [KO:K06583]
110563280  Itga6; integrin alpha-6 isoform X3 [KO:K06485]
110563345  Tnn; tenascin-N [KO:K06252]
110563491  Tnc; tenascin isoform X1 [KO:K06252]
110563512  Pik3r3; phosphatidylinositol 3-kinase regulatory subunit gamma isoform X1 [KO:K02649]
110564049  Thbs1; thrombospondin-1 isoform X1 [KO:K16857]
110564211  Rock1; rho-associated protein kinase 1 isoform X1 [KO:K04514] [EC:2.7.11.39]
110564898  Akt3; RAC-gamma serine/threonine-protein kinase isoform X1 [KO:K04456] [EC:2.7.11.1]
110564967  Lama4; laminin subunit alpha-4 [KO:K06241]
110565112  Pik3cd; phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform isoform X1 [KO:K00922] [EC:2.7.1.153]
110565123  Rhoa; transforming protein RhoA isoform X2 [KO:K04513]
110565216  Lamb2; laminin subunit beta-2 [KO:K06243]
110565312  Hgf; hepatocyte growth factor isoform X1 [KO:K05460]
110565673  Itga1; integrin alpha-1 isoform X1 [KO:K06480]
110566227  Src; proto-oncogene tyrosine-protein kinase Src isoform X2 [KO:K05704] [EC:2.7.10.2]
110566833  Arhgap5; rho GTPase-activating protein 5 isoform X1 [KO:K13709]
Compound
C04637  1-Phosphatidyl-D-myo-inositol 4,5-bisphosphate
C05981  Phosphatidylinositol-3,4,5-trisphosphate
Reference
PMID:14756624
  Authors
Berman AE, Kozlova NI, Morozevich GE.
  Title
Integrins: structure and signaling.
  Journal
Biochemistry (Mosc) 68:1284-99 (2003)
DOI:10.1023/b:biry.0000011649.03634.74
Reference
PMID:15179030
  Authors
Lee JW, Juliano R.
  Title
Mitogenic signal transduction by integrin- and growth factor receptor-mediated pathways.
  Journal
Mol Cells 17:188-202 (2004)
Reference
PMID:11573199
  Authors
Danen EH, Yamada KM.
  Title
Fibronectin, integrins, and growth control.
  Journal
J Cell Physiol 189:1-13 (2001)
DOI:10.1002/jcp.1137
Reference
PMID:15459662
  Authors
Guo W, Giancotti FG.
  Title
Integrin signalling during tumour progression.
  Journal
Nat Rev Mol Cell Biol 5:816-26 (2004)
DOI:10.1038/nrm1490
Reference
PMID:11229600
  Authors
Petit V, Thiery JP.
  Title
Focal adhesions: structure and dynamics.
  Journal
Biol Cell 92:477-94 (2000)
DOI:10.1016/S0248-4900(00)01101-1
Reference
PMID:12235283
  Authors
Stupack DG, Cheresh DA.
  Title
Get a ligand, get a life: integrins, signaling and cell survival.
  Journal
J Cell Sci 115:3729-38 (2002)
DOI:10.1242/jcs.00071
Reference
PMID:12640026
  Authors
Parsons JT.
  Title
Focal adhesion kinase: the first ten years.
  Journal
J Cell Sci 116:1409-16 (2003)
DOI:10.1242/jcs.00373
Reference
PMID:15688067
  Authors
Mitra SK, Hanson DA, Schlaepfer DD.
  Title
Focal adhesion kinase: in command and control of cell motility.
  Journal
Nat Rev Mol Cell Biol 6:56-68 (2005)
DOI:10.1038/nrm1549
Reference
PMID:14519391
  Authors
Comoglio PM, Boccaccio C, Trusolino L.
  Title
Interactions between growth factor receptors and adhesion molecules: breaking the rules.
  Journal
Curr Opin Cell Biol 15:565-71 (2003)
DOI:10.1016/S0955-0674(03)00096-6
Reference
PMID:15108811
  Authors
Schlaepfer DD, Mitra SK.
  Title
Multiple connections link FAK to cell motility and invasion.
  Journal
Curr Opin Genet Dev 14:92-101 (2004)
DOI:10.1016/j.gde.2003.12.002
Reference
PMID:14744429
  Authors
Burridge K, Wennerberg K.
  Title
Rho and Rac take center stage.
  Journal
Cell 116:167-79 (2004)
DOI:10.1016/S0092-8674(04)00003-0
Reference
PMID:11082269
  Authors
Schmitz AA, Govek EE, Bottner B, Van Aelst L.
  Title
Rho GTPases: signaling, migration, and invasion.
  Journal
Exp Cell Res 261:1-12 (2000)
DOI:10.1006/excr.2000.5049
Reference
PMID:10698680
  Authors
Vanhaesebroeck B, Alessi DR.
  Title
The PI3K-PDK1 connection: more than just a road to PKB.
  Journal
Biochem J 346 Pt 3:561-76 (2000)
DOI:10.1042/bj3460561
Reference
PMID:11882383
  Authors
Nicholson KM, Anderson NG.
  Title
The protein kinase B/Akt signalling pathway in human malignancy.
  Journal
Cell Signal 14:381-95 (2002)
DOI:10.1016/S0898-6568(01)00271-6
Reference
PMID:15053919
  Authors
Oloumi A, McPhee T, Dedhar S.
  Title
Regulation of E-cadherin expression and beta-catenin/Tcf transcriptional activity by the integrin-linked kinase.
  Journal
Biochim Biophys Acta 1691:1-15 (2004)
DOI:10.1016/j.bbamcr.2003.12.002
Reference
PMID:11146675
  Authors
Turner CE.
  Title
Paxillin and focal adhesion signalling.
  Journal
Nat Cell Biol 2:E231-6 (2000)
DOI:10.1038/35046659
Reference
PMID:15034601
  Authors
Hlobilkova A, Knillova J, Bartek J, Lukas J, Kolar Z.
  Title
The mechanism of action of the tumour suppressor gene PTEN.
  Journal
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 147:19-25 (2003)
Related
pathway
mun04010  MAPK signaling pathway
mun04060  Cytokine-cytokine receptor interaction
mun04070  Phosphatidylinositol signaling system
mun04110  Cell cycle
mun04151  PI3K-Akt signaling pathway
mun04310  Wnt signaling pathway
mun04512  ECM-receptor interaction
mun04810  Regulation of actin cytoskeleton
KO pathway
ko04510   

DBGET integrated database retrieval system