KEGG   PATHWAY: osa03030
osa03030                    Pathway                                
DNA replication - Oryza sativa japonica (Japanese rice) (RefSeq)
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
Pathway map
osa03030  DNA replication

Oryza sativa japonica (Japanese rice) (RefSeq) [GN:osa]
4326564  single-stranded DNA-binding protein, mitochondrial [KO:K03111]
4344789  DNA polymerase I A, chloroplastic [KO:K02335] [EC:]
4344791  DNA polymerase I B, mitochondrial isoform X1 [KO:K02335] [EC:]
4337150  DNA polymerase I A, chloroplastic-like isoform X1 [KO:K02335] [EC:]
4330902  replication protein A 70 kDa DNA-binding subunit A [KO:K07466]
4338202  uncharacterized protein At4g28440 [KO:K07466]
4326359  uncharacterized protein At4g28440 [KO:K07466]
4337594  replication protein A 70 kDa DNA-binding subunit C [KO:K07466]
4325130  uncharacterized protein At4g28440 [KO:K07466]
4332052  replication protein A 70 kDa DNA-binding subunit B [KO:K07466]
9267714  uncharacterized protein At4g28440 [KO:K07466]
4327373  replication protein A 70 kDa DNA-binding subunit B-like [KO:K07466]
4334834  replication protein A 70 kDa DNA-binding subunit B isoform X1 [KO:K07466]
4348221  replication protein A 70 kDa DNA-binding subunit B [KO:K07466]
9266301  uncharacterized protein LOC9266301 [KO:K07466]
4342616  replication protein A 70 kDa DNA-binding subunit isoform X1 [KO:K07466]
107280671  replication protein A 70 kDa DNA-binding subunit-like [KO:K07466]
9272338  replication protein A 70 kDa DNA-binding subunit [KO:K07466]
107279348  replication protein A 70 kDa DNA-binding subunit D-like isoform X1 [KO:K07466]
4345008  uncharacterized protein LOC4345008 isoform X1 [KO:K07466]
4351558  replication protein A 70 kDa DNA-binding subunit B-like [KO:K07466]
9266862  replication protein A 70 kDa DNA-binding subunit D isoform X1 [KO:K07466]
4331062  proliferating cell nuclear antigen [KO:K04802]
4336807  DNA replication ATP-dependent helicase/nuclease DNA2 [KO:K10742] [EC: 3.1.-.-]
4339464  flap endonuclease 1-A [KO:K04799] [EC:3.1.-.-]
4334691  flap endonuclease 1-B [KO:K04799] [EC:3.1.-.-]
4348965  DNA ligase 1 [KO:K10747] [EC:]
4324149  DNA ligase 3 [KO:K10747] [EC:]
4325052  DNA polymerase alpha catalytic subunit [KO:K02320] [EC:]
4351872  DNA polymerase alpha subunit B [KO:K02321]
9267485  DNA primase small subunit [KO:K02684] [EC:]
4342990  probable DNA primase large subunit [KO:K02685]
4349968  DNA polymerase delta catalytic subunit [KO:K02327] [EC:]
4331489  DNA polymerase delta small subunit [KO:K02328]
4327080  protein IWS1 homolog A [KO:K03504]
4347604  uncharacterized protein LOC4347604 [KO:K03505]
4344737  DNA polymerase delta subunit 4 [KO:K03505]
4329458  DNA polymerase epsilon catalytic subunit A isoform X1 [KO:K02324] [EC:]
4337886  DNA polymerase epsilon subunit 2 [KO:K02325] [EC:]
4347894  DNA polymerase epsilon subunit 3 [KO:K02326] [EC:]
4350526  DNA replication licensing factor MCM2 [KO:K02540] [EC:]
4339099  DNA replication licensing factor MCM3 [KO:K02541] [EC:]
107275673  DNA replication licensing factor MCM4 [KO:K02212] [EC:]
4331016  DNA replication licensing factor MCM5 [KO:K02209] [EC:]
4338176  DNA replication licensing factor MCM6 [KO:K02542] [EC:]
4352514  DNA replication licensing factor MCM7 [KO:K02210] [EC:]
4331236  replication protein A 32 kDa subunit A [KO:K10739]
4330068  replication protein A 32 kDa subunit B isoform X2 [KO:K10739]
4327706  replication protein A 14 kDa subunit [KO:K10740]
4350754  replication factor C subunit 1 [KO:K10754]
4351654  replication factor C subunit 2 [KO:K10755]
4336704  replication factor C subunit 4 [KO:K10755]
4330895  replication factor C subunit 3 [KO:K10756]
4334389  replication factor C subunit 5 [KO:K10756]
4349480  replication factor C subunit 3 [KO:K10756]
4349811  ribonuclease H2 subunit A [KO:K10743] [EC:]
9266900  ribonuclease H2 subunit B [KO:K10744]
4328433  ribonuclease H2 subunit C [KO:K10745]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
KO pathway

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