Entry
Name
Spliceosome - Etheostoma spectabile (orangethroat darter)
Description
After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.
Class
Genetic Information Processing; Transcription
BRITE hierarchy
Pathway map
Other DBs
Organism
Etheostoma spectabile (orangethroat darter) [GN:
esp ]
Gene
116669723 srsf5; serine/arginine-rich splicing factor 5 isoform X1 [KO:K12893 ]
116672292 puf60; poly(U)-binding-splicing factor PUF60 isoform X1 [KO:K12838 ]
116672296 u2surp; U2 snRNP-associated SURP motif-containing protein isoform X1 [KO:K12842 ]
116672732 srsf7; serine/arginine-rich splicing factor 7 isoform X1 [KO:K12896 ]
116679468 rbmx2; RNA-binding motif protein, X-linked 2 isoform X1 [KO:K13107 ]
116686636 snrnp200; LOW QUALITY PROTEIN: U5 small nuclear ribonucleoprotein 200 kDa helicase [KO:K12854 ] [EC:5.6.2.6 ]
116688852 usp39; U4/U6.U5 tri-snRNP-associated protein 2 isoform X1 [KO:K12847 ]
116688905 hnrnpk; heterogeneous nuclear ribonucleoprotein K isoform X1 [KO:K12886 ]
116688957 snrnp27; U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein isoform X1 [KO:K12846 ]
116689238 dhx38; pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1 [KO:K12815 ] [EC:5.6.2.6 ]
116690354 sf3b1; LOW QUALITY PROTEIN: splicing factor 3B subunit 1 [KO:K12828 ]
116691543 u2af2; splicing factor U2AF 65 kDa subunit isoform X1 [KO:K12837 ]
116692883 gpkow; G-patch domain and KOW motifs-containing protein isoform X1 [KO:K13101 ]
116695785 lsm2; U6 snRNA-associated Sm-like protein LSm2 isoform X1 [KO:K12621 ]
116696579 ncbp1; nuclear cap-binding protein subunit 1 isoform X1 [KO:K12882 ]
116697060 rbmx; RNA-binding motif protein, X chromosome isoform X1 [KO:K12885 ]
116698323 prpf40a; pre-mRNA-processing factor 40 homolog A isoform X1 [KO:K12821 ]
116700480 tcerg1; transcription elongation regulator 1 isoform X1 [KO:K12824 ]
116701739 tra2a; transformer-2 protein homolog alpha isoform X1 [KO:K12897 ]
116701748 snrnp40; U5 small nuclear ribonucleoprotein 40 kDa protein [KO:K12857 ]
116701891 srsf10; serine/arginine-rich splicing factor 10 isoform X1 [KO:K12900 ]
116703165 eftud2; 116 kDa U5 small nuclear ribonucleoprotein component [KO:K12852 ]
116704982 smndc1; survival of motor neuron-related-splicing factor 30 [KO:K12839 ]
Reference
Authors
Wahl MC, Will CL, Luhrmann R
Title
The spliceosome: design principles of a dynamic RNP machine.
Journal
Reference
Authors
Morris DP, Greenleaf AL
Title
The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Journal
Reference
Authors
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Title
Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Journal
Reference
Authors
Ritchie DB, Schellenberg MJ, MacMillan AM
Title
Spliceosome structure: piece by piece.
Journal
Reference
Authors
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Journal
Reference
Authors
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Title
Structure of the human activated spliceosome in three conformational states.
Journal
Reference
Authors
Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.
Journal
Reference
Authors
Zhan X, Lu Y, Shi Y
Title
Molecular basis for the activation of human spliceosome.
Journal
KO pathway