KEGG PATHWAY: ncar03040

KEGG

PATHWAY: ncar03040

Entry

ncar03040                   Pathway

Name

Spliceosome - Sciurus carolinensis (gray squirrel)

Description

After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.

Class

Genetic Information Processing; Transcription

Pathway map

Spliceosome

Other DBs

GO:

PDB:	5O9Z 5Z56 6FF7 8I0W

Organism

Sciurus carolinensis (gray squirrel) [GN:ncar]

Gene

[KO:K10599] [EC:2.3.2.27]

[KO:K12854] [EC:5.6.2.6]

[KO:K12815] [EC:5.6.2.6]

[KO:K12733] [EC:5.2.1.8]

[KO:K12823] [EC:5.6.2.7]

[KO:K12818] [EC:5.6.2.6]

[KO:K13025] [EC:5.6.2.7]

[KO:K12835] [EC:5.6.2.7]

[KO:K12811] [EC:5.6.2.7]

[KO:K12812] [EC:5.6.2.7]

[KO:K09567] [EC:5.2.1.8]

[KO:K12813] [EC:5.6.2.6]

[KO:K09564] [EC:5.2.1.8]

[KO:K12733] [EC:5.2.1.8]

[KO:K12820] [EC:5.6.2.6]

Reference

Authors

Wahl MC, Will CL, Luhrmann R

Title

The spliceosome: design principles of a dynamic RNP machine.

Journal

Cell 136:701-18 (2009)
DOI:10.1016/j.cell.2009.02.009

Reference

Authors

Morris DP, Greenleaf AL

Title

The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.

Journal

J Biol Chem 275:39935-43 (2000)
DOI:10.1074/jbc.M004118200

Reference

Authors

Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW

Title

Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.

Journal

Nucleic Acids Res 35:3928-44 (2007)
DOI:10.1093/nar/gkm347

Reference

Authors

Ritchie DB, Schellenberg MJ, MacMillan AM

Title

Spliceosome structure: piece by piece.

Journal

Biochim Biophys Acta 1789:624-33 (2009)
DOI:10.1016/j.bbagrm.2009.08.010

Reference

Authors

Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H

Title

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.

Journal

Cell 170:701-713.e11 (2017)
DOI:10.1016/j.cell.2017.07.011

Reference

Authors

Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y

Title

Structure of the human activated spliceosome in three conformational states.

Journal

Cell Res 28:307-322 (2018)
DOI:10.1038/cr.2018.14

Reference

Authors

Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H

Title

Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.

Journal

Cell 172:454-464.e11 (2018)
DOI:10.1016/j.cell.2018.01.010

Reference

Authors

Zhan X, Lu Y, Shi Y

Title

Molecular basis for the activation of human spliceosome.

Journal

Nat Commun 15:6348 (2024)
DOI:10.1038/s41467-024-50785-0

KO pathway

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