Entry
Name
Spliceosome - Salvelinus fontinalis (brook trout)
Description
After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.
Class
Genetic Information Processing; Transcription
BRITE hierarchy
Pathway map
Other DBs
Organism
Salvelinus fontinalis (brook trout) [GN:
sfon ]
Gene
129811507 hnrnpua; heterogeneous nuclear ribonucleoprotein U isoform X1 [KO:K12888 ]
129815705 snrpb; small nuclear ribonucleoprotein-associated proteins B and B' [KO:K11086 ]
129818363 snrpd3l; small nuclear ribonucleoprotein D3 polypeptide, like [KO:K11088 ]
129819880 snrnp40; U5 small nuclear ribonucleoprotein 40 kDa protein [KO:K12857 ]
129825517 srsf5b; serine and arginine rich splicing factor 5b isoform X1 [KO:K12893 ]
129826545 snrnp27; U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [KO:K12846 ]
129832461 taf15; TATA-binding protein-associated factor 2N isoform X1 [KO:K13098 ]
129835223 acin1b; apoptotic chromatin condensation inducer 1b isoform X1 [KO:K12875 ]
129836370 smndc1; survival of motor neuron-related-splicing factor 30 [KO:K12839 ]
129867294 snu13a; SNU13 homolog, small nuclear ribonucleoprotein a (U4/U6.U5) [KO:K12845 ]
129869395 ncbp2; nuclear cap-binding protein subunit 2 isoform X1 [KO:K12883 ]
Reference
Authors
Wahl MC, Will CL, Luhrmann R
Title
The spliceosome: design principles of a dynamic RNP machine.
Journal
Reference
Authors
Morris DP, Greenleaf AL
Title
The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Journal
Reference
Authors
Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Title
Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Journal
Reference
Authors
Ritchie DB, Schellenberg MJ, MacMillan AM
Title
Spliceosome structure: piece by piece.
Journal
Reference
Authors
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Journal
Reference
Authors
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Title
Structure of the human activated spliceosome in three conformational states.
Journal
Reference
Authors
Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Title
Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.
Journal
Reference
Authors
Zhan X, Lu Y, Shi Y
Title
Molecular basis for the activation of human spliceosome.
Journal
KO pathway