KEGG   ORTHOLOGY: K00070Help
Entry
K00070                      KO                                     

Name
HSD3B
Definition
3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]
Pathway
ko00140  Steroid hormone biosynthesis
ko01100  Metabolic pathways
ko04913  Ovarian steroidogenesis
ko04925  Aldosterone synthesis and secretion
ko04927  Cortisol synthesis and secretion
ko04934  Cushing syndrome
Module
M00107  Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
M00110  C19/C18-Steroid hormone biosynthesis, pregnenolone => androstenedione => estrone
Disease
H00216  Congenital adrenal hyperplasia (CAH)
H00599  46,XX disorders of sex development (Disorders related to androgen excess)
H00608  46,XY disorders of sex development (Disorders in androgen synthesis or action)
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00140 Steroid hormone biosynthesis
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 09150 Organismal Systems
  09152 Endocrine system
   04913 Ovarian Steroidogenesis
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
   04925 Aldosterone synthesis and secretion
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
   04927 Cortisol synthesis and secretion
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 09160 Human Diseases
  09166 Endocrine and metabolic diseases
   04934 Cushing syndrome
    K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
KEGG modules [BR:ko00002]
 Pathway module
  Carbohydrate and lipid metabolism
   Sterol biosynthesis
    M00107  Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
    M00110  C19/C18-Steroid hormone biosynthesis, pregnenolone => androstenedione => estrone
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.145  3beta-hydroxy-Delta5-steroid dehydrogenase
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
 5. Isomerases
  5.3  Intramolecular oxidoreductases
   5.3.3  Transposing C=C bonds
    5.3.3.1  steroid Delta-isomerase
     K00070  HSD3B; 3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
BRITE hierarchy
Other DBs
RN: R01837 R02216 R02499 R02840 R03327 R04163 R04678 R04849
GO: 0003854 0004769
Genes
HSA: 3283(HSD3B1) 3284(HSD3B2)
PTR: 457165 469446
PPS: 100982464(HSD3B2) 100983009 100990187
GGO: 101141801 101146563(HSD3B2)
PON: 100451655 100452394
NLE: 100596087 100605995(HSD3B2) 100606563(HSD3B1) 100607955
MCC: 712686(HSD3B2) 713091(HSD3B1)
MCF: 102129497 102133592(HSD3B1) 102134747 102135110
CSAB: 103224100(HSD3B1) 103224104 103224107 103224109(HSD3B2)
RRO: 104654599 104654605
MMU: 100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
HGL: 101707488(Hsd3b1) 101707858
CFA: 483146(HSD3B2)
AML: 100464280(HSD3B1)
UMR: 103677123
ORO: 101378904
FCA: 100616070(HSD3B2)
PTG: 102957262
AJU: 106978496
BTA: 281824(HSD3B1)
BOM: 102274088
BIU: 109555859
PHD: 102328070
CHX: 100860869(3BHSD)
OAS: 100190978(HSD3B1)
SSC: 445539(HSD3B1)
CFR: 102512447
CDK: 105089475
BACU: 103015346
LVE: 103083046
OOR: 101284283
ECB: 100034078(HSD3B2)
EPZ: 103548379
EAI: 106832920(HSD3B2)
MYB: 106723550(HSD3B2)
HAI: 109389868(HSD3B2)
RSS: 109446820(HAO2)
PALE: 102891879
LAV: 100659049(HSD3B2)
TMU: 101358325
MDO: 100012668
SHR: 100913530(HSD3B2)
OAA: 100080135
GGA: 396015(HSD3B1)
MGP: 104915630
CJO: 107319373(3beta-HSD)
ACYG: 106045741
TGU: 751770(HSD3B1)
GFR: 102039409
FAB: 101817697
PHI: 102101526
PMAJ: 107211459
CCW: 104687106
FPG: 101921564
FCH: 102052697
CLV: 102087460
EGZ: 104126518
AAM: 106497227
ASN: 102367786(HSD3B1)
AMJ: 109280168(HSD3B1)
PSS: 102453952
CMY: 102934871
CPIC: 101946936
ACS: 100561899(hsd3b1)
PVT: 110076926
PBI: 103067418
GJA: 107114856
XLA: 734818(hsd3b1.L)
XTR: 100490622
NPR: 108802532
DRE: 373131(hsd3b2) 553532(hsd3b1)
SRX: 107712267
SANH: 107658243
SGH: 107569293
CCAR: 109052439
IPU: 100304475(HSD3B)
AMEX: 103027852(hsd3b1)
TRU: 101069884(hsd3b1)
LCO: 104921488(hsd3b1)
MZE: 101485719
OLA: 100144358(3bhsd)
XMA: 102228303
PRET: 103477979
NFU: 107389612(hsd3b1)
CSEM: 103391994(hsd3b1)
LCF: 108902437
HCQ: 109522071
BPEC: 110156192(hsd3b1)
SASA: 106586600
ELS: 105016430
SFM: 108937872
LCM: 102345588
CMK: 103180179
DSI: Dsimw501_GD14693(Dsim_GD14693)
MDE: 101899660
AAG: 5566270
NVI: 100120136
ZNE: 110831195
FCD: 110862702
TUT: 107363285
TSP: Tsp_09898
OBI: 106875030
 » show all
TaxonomyKoalaUniProt
Reference
PMID:2243100
  Authors
Lachance Y, Luu-The V, Labrie C, Simard J, Dumont M, de Launoit Y, Guerin S, Leblanc G, Labrie F
  Title
Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells.
  Journal
J Biol Chem 265:20469-75 (1990)
  Sequence
[hsa:3283] [rno:360348 682974]
LinkDB All DBs

KEGG   ENZYME: 1.1.1.145Help
Entry
EC 1.1.1.145                Enzyme                                 

Name
3beta-hydroxy-Delta5-steroid dehydrogenase;
progesterone reductase;
Delta5-3beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene steroid dehydrogenase;
3beta-hydroxy steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid dehydrogenase/isomerase;
3beta-hydroxy-Delta5-C27-steroid oxidoreductase;
3beta-hydroxy-5-ene-steroid oxidoreductase;
steroid-Delta5-3beta-ol dehydrogenase;
3beta-HSDH;
5-ene-3-beta-hydroxysteroid dehydrogenase;
3beta-hydroxy-5-ene-steroid dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
3beta-hydroxy-Delta5-steroid:NAD+ 3-oxidoreductase
Reaction(IUBMB)
a 3beta-hydroxy-Delta5-steroid + NAD+ = a 3-oxo-Delta5-steroid + NADH + H+ [RN:R03176]
Reaction(KEGG)
Substrate
3beta-hydroxy-Delta5-steroid [CPD:C03836];
NAD+ [CPD:C00003]
Product
3-oxo-Delta5-steroid [CPD:C01034];
NADH [CPD:C00004];
H+ [CPD:C00080]
Comment
This activity is found in several bifunctional enzymes that catalyse the oxidative conversion of Delta5-3-hydroxy steroids to a Delta4-3-oxo configuration. This conversion is carried out in two separate, sequential reactions; in the first reaction, which requires NAD+, the enzyme catalyses the dehydrogenation of the 3beta-hydroxy steroid to a 3-oxo intermediate. In the second reaction the reduced coenzyme, which remains attached to the enzyme, activates the isomerization of the Delta5 form to a Delta4 form (cf. EC 5.3.3.1, steroid Delta-isomerase). Substrates include dehydroepiandrosterone (which is converted into androst-5-ene-3,17-dione), pregnenolone (converted to progesterone) and cholest-5-en-3-one, an intermediate of cholesterol degradation.
History
EC 1.1.1.145 created 1972
Pathway
ec00140  Steroid hormone biosynthesis
ec00984  Steroid degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
K16045  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase
Genes
HSA: 3283(HSD3B1) 3284(HSD3B2)
PTR: 457165 469446
PPS: 100982464(HSD3B2) 100983009 100990187
GGO: 101141801 101146563(HSD3B2)
PON: 100451655 100452394
NLE: 100596087 100605995(HSD3B2) 100606563(HSD3B1) 100607955
MCC: 712686(HSD3B2) 713091(HSD3B1)
MCF: 102129497 102133592(HSD3B1) 102134747 102135110
CSAB: 103224100(HSD3B1) 103224104 103224107 103224109(HSD3B2)
RRO: 104654599 104654605
MMU: 100043456(Gm10681) 15492(Hsd3b1) 15493(Hsd3b2) 15495(Hsd3b4) 15496(Hsd3b5) 15497(Hsd3b6)
RNO: 108348086 24470(Hsd3b5) 29632(Hsd3b2) 360348(Hsd3b1) 682974(Hsd3b3)
HGL: 101707488(Hsd3b1) 101707858
CFA: 483146(HSD3B2)
AML: 100464280(HSD3B1)
UMR: 103677123
ORO: 101378904
FCA: 100616070(HSD3B2)
PTG: 102957262
AJU: 106978496
BTA: 281824(HSD3B1)
BOM: 102274088
BIU: 109555859
PHD: 102328070
CHX: 100860869(3BHSD)
OAS: 100190978(HSD3B1)
SSC: 445539(HSD3B1)
CFR: 102512447
CDK: 105089475
BACU: 103015346
LVE: 103083046
OOR: 101284283
ECB: 100034078(HSD3B2)
EPZ: 103548379
EAI: 106832920(HSD3B2)
MYB: 106723550(HSD3B2)
HAI: 109389868(HSD3B2)
RSS: 109446820(HAO2)
PALE: 102891879
LAV: 100659049(HSD3B2)
TMU: 101358325
MDO: 100012668
SHR: 100913530(HSD3B2)
OAA: 100080135
GGA: 396015(HSD3B1)
MGP: 104915630
CJO: 107319373(3beta-HSD)
ACYG: 106045741
TGU: 751770(HSD3B1)
GFR: 102039409
FAB: 101817697
PHI: 102101526
PMAJ: 107211459
CCW: 104687106
FPG: 101921564
FCH: 102052697
CLV: 102087460
EGZ: 104126518
AAM: 106497227
ASN: 102367786(HSD3B1)
AMJ: 109280168(HSD3B1)
PSS: 102453952
CMY: 102934871
CPIC: 101946936
ACS: 100561899(hsd3b1)
PVT: 110076926
PBI: 103067418
GJA: 107114856
XLA: 734818(hsd3b1.L)
XTR: 100490622
NPR: 108802532
DRE: 373131(hsd3b2) 553532(hsd3b1)
SRX: 107712267
SANH: 107658243
SGH: 107569293
CCAR: 109052439
IPU: 100304475(HSD3B)
AMEX: 103027852(hsd3b1)
TRU: 101069884(hsd3b1)
LCO: 104921488(hsd3b1)
MZE: 101485719
OLA: 100144358(3bhsd)
XMA: 102228303
PRET: 103477979
NFU: 107389612(hsd3b1)
CSEM: 103391994(hsd3b1)
LCF: 108902437
HCQ: 109522071
BPEC: 110156192(hsd3b1)
SASA: 106586600
ELS: 105016430
SFM: 108937872
LCM: 102345588
CMK: 103180179
DSI: Dsimw501_GD14693(Dsim_GD14693)
MDE: 101899660
AAG: 5566270
NVI: 100120136
ZNE: 110831195
FCD: 110862702
TUT: 107363285
TSP: Tsp_09898
OBI: 106875030
MTU: Rv1106c
MTC: MT1137
MRA: MRA_1117
MTUR: CFBS_1174
MTD: UDA_1106c
MTUC: J113_07785
MTUE: J114_05980
MTUH: I917_07855
MTUL: TBHG_01091
MTUT: HKBT1_1172
MTUU: HKBT2_1178
MBB: BCG_1166c
MBT: JTY_1139
MBX: BCGT_0934
MAF: MAF_11210
MMIC: RN08_1242
MLE: ML1942
MLB: MLBr01942
MPA: MAP_2688
MAO: MAP4_1128
MAVI: RC58_05605
MAVU: RE97_05600
MAV: MAV_1225
MAVD: NF84_05415
MAVR: LA63_05535
MAVA: LA64_05550
MIT: OCO_11340
MIA: OCU_11320
MID: MIP_01824
MYO: OEM_11450
MIR: OCQ_11350
MUL: MUL_0172
MMC: Mmcs_4109
MKM: Mkms_4185
MJL: Mjls_4340
MMI: MMAR_4359
MMM: W7S_05545
MHAD: B586_07090
MVA: Mvan_4635
MGI: Mflv_2075
MVQ: MYVA_4444
 » show all
Taxonomy
Reference
1  [PMID:4226148]
  Authors
Cheatum SG, Watten JC.
  Title
Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.
  Journal
Biochim Biophys Acta 122:1-13 (1966)
Reference
2  [PMID:14220672]
  Authors
KORITZ SB.
  Title
THE CONVERSION OF PREGNENOLONE TO PROGESTERONE BY SMALL PARTICLES FROM RAT ADRENAL.
  Journal
Biochemistry 3:1098-102 (1964)
Reference
3
  Authors
Neville, A.M., Orr, J.C. and Engel, L.L.
  Title
Delta5-3beta-Hydroxy steroid dehydrogenase activities of bovine adrenal cortex.
  Journal
Biochem J 107:20 (1968)
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.145
IUBMB Enzyme Nomenclature: 1.1.1.145
ExPASy - ENZYME nomenclature database: 1.1.1.145
BRENDA, the Enzyme Database: 1.1.1.145
CAS: 9044-85-3
LinkDB All DBs

KEGG   REACTION: R02216Help
Entry
R02216                      Reaction                               

Name
Pregnenolone:NAD+ 3-oxidoreductase
Definition
Pregnenolone + NAD+ <=> Progesterone + NADH + H+
Equation
Comment
3beta-hydroxysteroid dehydrogenase/ Delta5-Delta4-isomerase
two-step reaction
Reaction class
RC00001  C00003_C00004
RC00146  C00410_C01953
Enzyme
1.1.1.145       5.3.3.1
Pathway
rn00140  Steroid hormone biosynthesis
rn01100  Metabolic pathways
Module
M00107  Steroid hormone biosynthesis, cholesterol => prognenolone => progesterone
Orthology
K00070  3beta-hydroxy-Delta5-steroid dehydrogenase / steroid Delta-isomerase [EC:1.1.1.145 5.3.3.1]
K01822  steroid Delta-isomerase [EC:5.3.3.1]
LinkDB All DBs

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