Entry Name Spliceosome - Oncorhynchus mykiss (rainbow trout)
Description After transcription, eukaryotic mRNA precursors contain protein-coding exons and noncoding introns. In the following splicing, introns are excised and exons are joined by a macromolecular complex, the spliceosome. The standard spliceosome is made up of five small nuclear ribonucleoproteins (snRNPs), U1, U2, U4, U5, and U6 snRNPs, and several spliceosome-associated proteins (SAPs). Spliceosomes are not a simple stable complex, but a dynamic family of particles that assemble on the mRNA precursor and help fold it into a conformation that allows transesterification to proceed. Various spliceosome forms (e.g. A-, B- and C-complexes) have been identified.
Class Genetic Information Processing; TranscriptionBRITE hierarchy
Pathway map Other DBs Organism Oncorhynchus mykiss (rainbow trout) [GN:
omy ]
Gene 100301691 hspa8b; heat shock protein family A (Hsp70) member 8b [KO:K03283 ]
110486221 eftud2; 116 kDa U5 small nuclear ribonucleoprotein component [KO:K12852 ]
110494224 hnrnpa1a; heterogeneous nuclear ribonucleoprotein A1a isoform X2 [KO:K12741 ]
110496847 snrnp40; U5 small nuclear ribonucleoprotein 40 kDa protein isoform X1 [KO:K12857 ]
110498097 hnrnpua; heterogeneous nuclear ribonucleoprotein U isoform X1 [KO:K12888 ]
110503518 taf15; TATA-binding protein-associated factor 2N isoform X1 [KO:K13098 ]
110506516 dhx38; pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16 isoform X1 [KO:K12815 ] [EC:5.6.2.6 ]
110508482 acin1a; apoptotic chromatin condensation inducer in the nucleus [KO:K12875 ]
110516093 dhx16; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 isoform X1 [KO:K12813 ] [EC:5.6.2.6 ]
110523885 hnrpkl; heterogeneous nuclear ribonucleoprotein K, like isoform X1 [KO:K12886 ]
110528556 snrpb; small nuclear ribonucleoprotein-associated proteins B and B' [KO:K11086 ]
110529920 puf60a; poly(U)-binding-splicing factor PUF60a isoform X1 [KO:K12838 ]
110531633 acin1b; apoptotic chromatin condensation inducer in the nucleus isoform X1 [KO:K12875 ]
110531756 dhx15; pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 isoform X1 [KO:K12820 ] [EC:5.6.2.6 ]
110536249 snrnp27; U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein [KO:K12846 ]
110537051 u2af2a; splicing factor U2AF 65 kDa subunit isoform X1 [KO:K12837 ]
Reference Authors Wahl MC, Will CL, Luhrmann R
Title The spliceosome: design principles of a dynamic RNP machine.
Journal Reference Authors Morris DP, Greenleaf AL
Title The splicing factor, Prp40, binds the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Journal Reference Authors Chen YI, Moore RE, Ge HY, Young MK, Lee TD, Stevens SW
Title Proteomic analysis of in vivo-assembled pre-mRNA splicing complexes expands the catalog of participating factors.
Journal Reference Authors Ritchie DB, Schellenberg MJ, MacMillan AM
Title Spliceosome structure: piece by piece.
Journal Reference Authors Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, Luhrmann R, Stark H
Title Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Journal Reference Authors Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Title Structure of the human activated spliceosome in three conformational states.
Journal Reference Authors Haselbach D, Komarov I, Agafonov DE, Hartmuth K, Graf B, Dybkov O, Urlaub H, Kastner B, Luhrmann R, Stark H
Title Structure and Conformational Dynamics of the Human Spliceosomal B(act) Complex.
Journal Reference Authors Zhan X, Lu Y, Shi Y
Title Molecular basis for the activation of human spliceosome.
Journal KO pathway
